Half-lives and relative amounts of stored and polysomal ribosomes and poly(A)+ RNA in mouse oocytes

Leon, Victor De; Johnson, Andrew C.; Bachvarova, Rosemary F.

doi:10.1016/0012-1606(83)90369-x

Cited by 84 publications

(57 citation statements)

References 22 publications

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“…Depending on the species and stage, poly(A) RNA comprises approximately 10 to 20% of total RNA in oocytes [67,72] and declines by 30 to 50% during meiotic maturation [this study , 67, 72-74]. In the present study, heat stress exposure had no impact on the total amount of poly(A) RNA.…”

Section: Discussionmentioning

confidence: 45%

“…Considering that the cytoplasm and its components are almost entirely from the oocyte [70] and major embryonic genome activation has not yet occurred [71], it is not surprising that certain features of RNA from 4 to 8-cell embryos are similar to oocyte RNA. The blastocyst-stage embryo being largely comprised of trophectoderm cells (i.e., the first epithelium) provides some explanation as to why this RNA is similar to that obtained from the somatic-like cumulus cells.Depending on the species and stage, poly(A) RNA comprises approximately 10 to 20% of total RNA in oocytes [67,72] and declines by 30 to 50% during meiotic maturation [this study, 67, 72-74]. In the present study, heat stress exposure had no impact on the total amount of poly(A) RNA.…”

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confidence: 45%

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Impact of Heat Stress Exposure During Meiotic Maturation on Oocyte, Surrounding Cumulus Cell, and Embryo RNA Populations

et al. 2011

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Abstract. To determine if reductions in developmental competence related to heat stress exposure were correlated with perturbations in certain RNA populations, poly(A) RNA, total RNA, RNA size distribution, and the abundance of transcripts (cyclin B1, GDF9, BMP15, poly(A) polymerase, HSP70, 18S & 28S rRNA) were examined in oocytes matured at 38.5 or 41 C. Performing in vitro fertilization resulted in embryos for examining RNA. Relative to germinal vesiclestage oocytes, total amount of poly(A) RNA decreased similarly in oocytes matured at 38.5 or 41 C. Total RNA did not change during meiotic maturation or up through the 4 to 8-cell stage of embryonic development. Blastocyst-stage embryos had more total RNA; those originating from heat-stressed oocytes had more than those from nonheat-stressed oocytes. Oocytes and 4 to 8-cell embryos had similar RIN values and ratios for rRNA, 18S/fast region, and 18S/inter region. Values obtained for blastocyst-stage embryos were similar to those obtained for cumulus cell RNA, which did not change during maturation. Culture at 41 C for the first 12 h of meiotic maturation had no impact on RNA size distribution or transcripts examined from oocytes, surrounding cumulus or resultant 4 to 8-cell embryos. Interestingly, however, RNA from blastocysts originating from heat-stressed oocytes had lower 18S/fast region and 18S/inter region ratios compared to other developmental stages and cumulus cells. Although biological significance of these RNA changes is unclear, differences at the molecular level in embryos from heat-stressed oocytes emphasize the importance of minimizing stress exposure during meiotic maturation, if the intent is to obtain developmentally-competent embryos. Key words: Cumulus, Heat stress, Microcapillary electrophoresis, Oocyte, RNA (J. Reprod. Dev. 57: [481][482][483][484][485][486][487][488][489][490][491] 2011) griculturally-important females exposed to environmental heat stress experience reduced fertility [1][2][3][4]. In cattle, this is largely due to hyperthermia [5][6][7] whereby rectal temperatures may reach or exceed 41 C [5,[8][9][10][11][12]. Effects of environmental heat stress to reduce female fertility are pronounced during or near estrus [13][14][15]. This corresponds to the time period when the female is receptive to mating and the oocyte within the Graafian follicle resumes meiosis and progresses to metaphase II (MII; meiotic maturation) in preparation for fertilization. Hyperthermia during this time has been associated with reductions in early embryonic development [16] and increases in embryonic loss near the time of placental attachment [17].The negative impact of hyperthermia during estrus may be attributable to indirect effects on the maternal environment [reviewed by 18] and/or due to direct effects on the cumulus-oocyte complex. Direct exposure of the cumulus-oocyte complex to 41 C during meiotic maturation reduces early embryo development after fertilization by 30 to 65% [19][20][21][22][23][24][25] similar to consequences observed when e...

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Section: Discussionmentioning

confidence: 45%

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confidence: 45%

Impact of Heat Stress Exposure During Meiotic Maturation on Oocyte, Surrounding Cumulus Cell, and Embryo RNA Populations

et al. 2011

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“…Although the exact identity of most of these factors remains a mystery, they are nevertheless instrumental in preparing the oocyte for the egg to embryo transition. [1][2][3][4][5] The presence and correct storage of these maternal factors is crucial for embryonic genome activation (EGA) which needs to occur for proper embryonic development. 6,7 During this transition, mRNAs are released, translated, and the resulting proteins, along with other stored maternal factors, are targeted to the nucleus in a regulated fashion where they then orchestrate the nuclear reprogramming events leading to the EGA.…”

Section: Introductionmentioning

confidence: 99%

Role for PADI6 in securing the mRNA-MSY2 complex to the oocyte cytoplasmic lattices

Liu

Morency

et al. 2017

Cell Cycle

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The oocyte cytoplasmic lattices (CPLs) have long been predicted to function as a storage form for the maternal contribution of ribosomes to the early embryo. Our previous studies have demonstrated that ribosomal component S6 is stored in the oocyte CPLs and peptidylarginine deiminase 6 (PADI6) is critical for CPLs formation. Additionally, we found that depletion of PADI6 reduced de novo protein synthesis prior to the maternal-to-embryonic transition, therefore causing embryos to arrest at the 2-cell stage. Here, we present evidence further supporting the association of ribosomes with the CPLs by demonstrating that rRNAs are dramatically decreased in Padi6 KO oocytes. We also show that the abundance and localization of mRNAs is affected upon PADI6 depletion, suggesting that mRNAs are very possibly associated with CPLs. Consistent with this observation, the amount of the major RNA binding protein, MSY2, that is associated with the insoluble fraction of the oocytes after Triton X-100 extraction is also markedly decreased in the Padi6 KO oocytes. Furthermore, treatment of the oocytes with RNase A followed by Triton X-100 extraction severely impairs the localization of PADI6 and MSY2 in oocytes. These results indicate that mRNAs, possibly in a complex with MSY2 and PADI6, are bound in the CPLs and may play a role in securing the mRNA-MSY2 complex to the CPLs.

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“…They also maintain high transcriptional activity, expanding their cytoplasm as much as 500 fold as they accumulate mRNAs and proteins to be used by the embryo during early development. 110 Preparation for embryogenesis in C. elegans. C. elegans spermatogenic precursors complete meiosis during the last larval stage.…”

Section: Preparation For Embryogenesismentioning

confidence: 99%

“…In sexually mature mice, hormonally-triggered ovulation allows the oocyte to proceed from prophase I to metaphase II, with concurrent pausing of transcription and reduction of translation. 110 At fertilization, the egg completes meiosis, and the female pronucleus remains in a repressed state. In contrast, protamines in the male pronucleus are rapidly replaced by highly acetylated histones, producing an open chromatin state.…”

Section: Preparation For Embryogenesismentioning

confidence: 99%

Genetics of germ cell development

2012

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| The germ line represents a continuous cellular link between generations and between species, but the germ cells themselves develop in a specialized, organism-specific context. The three most common animal model organisms, C. elegans, Drosophila, and mouse, display striking similarities as well as major differences in the means by which they control germ cell development.Comparison of the germ cells of these three organisms sheds light not only on universal aspects of germline regulation, but also on control of the pluripotent state in vivo and on the earliest steps of embryogenesis. Taking a broad perspective, we will follow the germ cells from specification in the early embryo, through gametogenesis, to fertilization, and highlight themes from the comparison of three alternative strategies for navigating the fundamental cycle of sexual reproduction.

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Half-lives and relative amounts of stored and polysomal ribosomes and poly(A)+ RNA in mouse oocytes

Cited by 84 publications

References 22 publications

Impact of Heat Stress Exposure During Meiotic Maturation on Oocyte, Surrounding Cumulus Cell, and Embryo RNA Populations

Impact of Heat Stress Exposure During Meiotic Maturation on Oocyte, Surrounding Cumulus Cell, and Embryo RNA Populations

Role for PADI6 in securing the mRNA-MSY2 complex to the oocyte cytoplasmic lattices

Genetics of germ cell development

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