Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae

Abstract

Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae Shu-Kun Tang, 1 3 Xin-Peng Tian, 1,2 3 Xiao-Yang Zhi, 1 A novel halophilic, filamentous, actinomycete strain, designated YIM 90648 T , was isolated from a salt lake in Xinjiang Province, north-west China, and subjected to a polyphasic taxonomic study. Optimal growth occurred at 37 6C, pH 7.0-8.0 and 15 % (w/v) NaCl. The aerial mycelium of strain YIM 90648 T formed long chains of spores at maturity and the spores were cylindrical with smooth surfaces. Spore chains with pseudosporangia at the end were borne on the substrate mycelium and most spores had wrinkled surfaces. Strain YIM 90648 T contained mesodiaminopimelic acid as the diagnostic diamino acid and galactose and ribose as the major wholecell components. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol mannoside. MK-10(H 8 ), MK-11(H 4 ), MK-11(H 6 ) and MK-11(H 8 ) were the predominant menaquinones. The major fatty acids were i-C 16 : 0 and ai-C 17 : 0 . The DNA G+C content was 68 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 90648 T formed a distinct lineage within the family Nocardiopsaceae and showed 16S rRNA gene sequence similarity of 93.3-95.0 % with members of the family Nocardiopsaceae. On the basis of the polyphasic evidence, a novel genus and species, Haloactinospora alba gen. nov., sp. nov., is proposed to accommodate this isolate. The type strain of Haloactinospora alba is YIM 90648 T (5DSM 45015 T 5CCTCC AA 206008 T ).

Marinactinospora thermotolerans gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea

“…Differentiating chemotaxonomic characteristics of strain SCSIO 00652 T and recognized genera of the family Nocardiopsaceae Taxa: 1, strain SCSIO 00652 T ; 2, Thermobifida; 3, Nocardiopsis; 4, Haloactinospora; 5, Streptomonospora. Data are from this study, Cai et al (2008), Cui et al (2001), Kroppenstedt & Evtushenko (2006), Tang et al (2008), Yang et al (2008) and Zhang et al (1998). Cell walls of all taxa contain meso-diaminopimelic acid.…”

Saccharopolyspora qijiaojingensis sp. nov., a halophilic actinomycete isolated from a salt lake

“…Tang et al (2008). The strain was maintained on International Streptomyces Project (ISP) medium 4 agar slants containing 10 % (w/v) NaCl at 4 u C and as suspensions of mycelium fragments in glycerol (20 %, v/v).…”

Haloglycomyces albus gen. nov., sp. nov., a halophilic, filamentous actinomycete of the family Glycomycetaceae

“…During an investigation of actinomycete diversity in hypersaline habitats, strain YIM 92370 T was isolated from a soil sample collected from Xinjiang Province, north-west China, by using cellulose-casein medium with multi-salts as described by Tang et al (2008). The aim of this study was to identify the exact taxonomic status of strain YIM 92370 T by using a polyphasic taxonomy study.…”