Halofuginone infused keratin hydrogel attenuates adhesions in a rodent cecal abrasion model

Peyton, Charles C.; Keys, Tristan; Tomblyn, Seth; Burmeister, David M.; Beumer, Jan H.; Holleran, Juliane L.; Sirintrapun, Joseph S.; Washburn, Scott A.; Hodges, Steve J.

doi:10.1016/j.jss.2012.07.053

Cited by 32 publications

(23 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As compared to results recently published in the literature using Adept® (Baxter Healthcare Corporation, USA) [28,29,30,31,32,33], Interceed® (Ethicon, Inc., USA) [25,27,29,34,35,36,37,38,39], and Seprafilm® (Genzyme Corporation, USA) [26,27,31,34,38,39,40,41,42,43,44] in rat models, the results obtained with 4DryField PH in this study are in the upper range of benefit and are comparable with the data of Kaptanoglu et al [43] and Wallwiener et al [29]. …”

Section: Discussionmentioning

confidence: 96%

Evaluation of 4DryField® PH as Adhesion Prevention Barrier Tested in an Optimized Adhesion Model in Rats

Poehnert¹,

Abbas

Kreipe

et al. 2015

Eur Surg Res

View full text Add to dashboard Cite

Background: Adhesions due to pelvic/abdominal surgery are a common serious pathology possibly entailing severe complications. This study investigates the adhesion prevention capability of the novel starch-based agent 4DryField® PH, which together with saline solution forms a barrier gel. Herein, an optimized adhesion model (OPAM) inducing severe adhesions/agglutinations with high reproducibility was used. Methods: In 19 Lewis rats, a 1 × 2 cm abdominal wall defect was created, the peritoneum of the neighboring cecum was abraded, and both injured areas were approximated by suture. Rats were randomized to control (n = 10) or 4DryField PH treatment (n = 9) groups. Another 8 rats had sham surgery for safety assessment of 4DryField PH. At day 7, the quantity and quality of adhesions were assessed macro-/microscopically and evaluated statistically. Results: 4DryField PH treatment significantly reduced the incidence and severity of adhesions as verified by significantly improved adhesion scorings (0.4 vs. 4.5; 1.1 vs. 9). Histology revealed reconstitution of the cecum and abdominal wall including regeneration of the visceral/parietal peritoneum. In sham-operated rats, 4DryField PH did not induce adhesion formation. Conclusions: 4DryField PH gel was highly effective in preventing adhesions. Histologically, the injured cecum and abdominal wall regenerated well in the presence of 4DryField PH. Considering the severity of OPAM trauma, the potential of 4DryField PH to prevent adhesions can be rated excellent.

show abstract

Section: Discussionmentioning

confidence: 96%

Evaluation of 4DryField® PH as Adhesion Prevention Barrier Tested in an Optimized Adhesion Model in Rats

Poehnert¹,

Abbas

Kreipe

et al. 2015

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…The abdominal skin was prepared and disinfected with povidone-iodine prior to the procedure. As previously described in the literature [17], a 2-to 3-cm-long lower abdominal midline incision was used to access the abdominal cavity. The pouchlike cecum was located in the right iliac fossa.…”

Section: Animal and Establishment Of Intra-abdominal Adhesion Modelmentioning

confidence: 99%

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Wei

Chen

Wang

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.

show abstract

“…Compared with current barrier systems, hydrogels [41][42][43][44][45][46][47] have several unique physicochemical features. They can gelate in response to increasing temperature, transforming from a flowing solution at room temperature to a gel at body temperature, and then rapidly form a protective film covering the surface of peritoneal wounds to avoid formation of adhesions in the abdominal cavity.…”

Section: Discussionmentioning

confidence: 99%

Novel thermosensitive hydrogel for preventing formation of abdominal adhesions

Gao

Deng²,

Wei³

et al. 2013

IJN

View full text Add to dashboard Cite

Adhesions can form after almost any type of abdominal surgery. Postoperative adhesions can be prevented by improved surgical techniques, such as reducing surgical trauma, preventing ischemia, and avoiding exposure of the peritoneal cavity to foreign materials. Although improved surgical techniques can potentially reduce formation of adhesions, they cannot be eliminated completely. Therefore, finding more effective methods to prevent postoperative adhesions is imperative. Recently, we found that a novel thermosensitive hydrogel, ie, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC) had the potential to prevent postoperative adhesions. Using the ring-opening polymerization method, we prepared a PCEC copolymer which could be dissolved and assembled at 55°C into PCEC micelles with mean size of 25 nm. At body temperature, a solution containing PCEC micelles could convert into a hydrogel. The PCEC copolymer was biodegradable and had low toxicity in vitro and in vivo. We found that most animals in a hydrogel-treated group (n = 10) did not develop adhesions. In contrast, 10 untreated animals developed adhesions that could only be separated by sharp dissection (P , 0.001). The hydrogel could adhere to peritoneal wounds and degraded gradually over 7-9 days, transforming into a viscous fluid that was completely absorbed within 12 days. The injured parietal and visceral peritoneum remesothelialized over about seven and nine days, respectively. This study confirms that PCEC hydrogel has potential application in the prevention of postoperative adhesions.

show abstract

Halofuginone infused keratin hydrogel attenuates adhesions in a rodent cecal abrasion model

Cited by 32 publications

References 29 publications

Evaluation of 4DryField® PH as Adhesion Prevention Barrier Tested in an Optimized Adhesion Model in Rats

Evaluation of 4DryField® PH as Adhesion Prevention Barrier Tested in an Optimized Adhesion Model in Rats

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Novel thermosensitive hydrogel for preventing formation of abdominal adhesions

Contact Info

Product

Resources

About