Halomonas alkaliantarctica sp. nov., isolated from saline lake Cape Russell in Antarctica, an alkalophilic moderately halophilic, exopolysaccharide-producing bacterium

Poli, Annarita; Esposito, Enrico; Orlando, Pierangelo; Lama, Licia; Giordano, Assunta; Appolonia, Francesca de; Nicolaus, Barbara; Gambacorta, Agata

doi:10.1016/j.syapm.2006.03.003

Cited by 100 publications

(51 citation statements)

References 31 publications

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“…The phylogenetic analysis revealed that almost half of our strains grouped with different Halomonas species. These widely distributed species (characterized by special enzymic activity) are among the most frequently isolated aerobic or facultatively anaerobic bacteria from saline lakes and seawaters Kim et al 2007;Poli et al 2007). Analysis of clone libraries from the water of Lake Bonney, a chemically stratified, permanently ice-covered Antarctic lake, also revealed a dominant presence of Halomonas and halophilic Bacillus-like sequences (Glatz et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Diversity and activity of cultivable aerobic planktonic bacteria of a saline Lake located in Sovata, Romania

et al. 2010

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Aerobic bacterial strains from the salt water of Lake Red (Sovata, Romania) were cultivated. More than half of the 80 strains were G(-) and formed motile straight rods. Only a few strains produced acid from D-glucose and reduced nitrate to nitrite. Optimum NaCl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. On the basis of the 16S rRNA gene sequence similarity almost half of the strains were identified as members of genus Halomonas. Other strains belonged to genera Marinobacter, Psychrobacter, Serratia, Morganella (γ-Proteobacteria), Bacillus, Exiguobacterium, Planococcus (Firmicutes), and Arthrobacter, Micrococcus, Microbacterium, and Nesterenkonia (Actinobacteria).

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Section: Resultsmentioning

confidence: 99%

Diversity and activity of cultivable aerobic planktonic bacteria of a saline Lake located in Sovata, Romania

et al. 2010

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“…Indeed, the genus Halomonas has received increasing interest, as several Halomonas species are able to produce significant quantities of EPS with high surface activity and/or rheological properties (Martinez-Checa et al, 2002). Some species belonging to the genus Halomonas are able to produce EPSs: H. rifensis (Amjres et al, 2011), H. maura (Arias et al, 2003), H. alkaliphila (Romano et al, 2006), H. alkaliantarctica (Poli et al, 2004(Poli et al, , 2007 (Wu, Y.H. et al, 2008), H. daqingensis (Wu, G. et al, 2008), H. eurihalina (Martínez-Checa et al, 2002), H. fontilapidosi (Gonzalez-Domenech et al, 2009), and H. sabkhae (Kharroub et al, 2008).…”

Section: Eps Produced By Extremophilic Bacteriamentioning

confidence: 99%

“…strain AAD6 could represent an alternative cheap source of levan polymer when grown on definite media, hypothesizing its larger employment in industrial applications as a nonpathogenic microorganism (Kuçukasik et al, 2011), as a bioflocculant agent in the treatment of industrial wastewaters (Sam et al, 2011), and in a nanocarrier system for peptide and protein drug delivery (Sezer et al, 2011) according to its high film-forming capability (Sima et al, 2011). H. alkaliantarctica strain CRSS was isolated from salt sediments near the salt lake at Cape Russell, Antarctica (Poli et al, 2004(Poli et al, , 2007. The strain CRSS grew aerobically in a complex medium containing 100 g/L NaCl.…”

Section: Eps Produced By Extremophilic Bacteriamentioning

confidence: 99%

Bacterial Polymers Produced by Extremophiles: Biosynthesis, Characterization, and Applications of Exopolysaccharides

2012

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“…When growth medium was amended with sucrose, the highest EPS yield was obtained from Hahella chejuensis isolated from Cheju Island, Republic of Korea [27]. The haloalkaliphilic microorganism, Halomonas alkaliantartica strain CRSS which was isolated from a saline lake in Antarctica produced maximum EPS when acetate was used as a carbon source [28]. EPS production requires high carbon content and less nitrogen quantity in the production medium [29].…”

Section: Introductionmentioning

confidence: 99%

“…Halomonas is the potential genus producing EPS having efficient emulsifying activity among halophilic bacteria. Halomonas alkaliantartica, H. ventose, H. anticariencis and H. maura were found to produce EPS that are proposed to have a role as an emulsifying agent in oil recovery [36,28,23,32].…”

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Exopolysaccharide From Marine Moderately Halophilic Bacterium Halomonas Smyrnensis SVD Iii

Deshmukh

Kanekar²,

Bhadekar

2017

Int J Pharm Pharm Sci

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Objective: To study 1) Optimization of nutritional and environmental parameters to enhance the yield of EPS by Halomonas smyrnensis SVD III isolated from seawater, West Coast of Maharashtra, India and 2) Purification and characterization of the EPS produced. Methods:The isolate was grown in Sehgal and Gibbons (SG) medium broth supplemented with 3% glucose, at 37 °C, 120 rpm for 7 d. Optimization of different parameters was carried out with one factor at a time approach. EPS was isolated from cell-free supernatant of the culture broth by centrifugation and precipitation using chilled ethanol, after removal of proteins by trichloroacetic acid (TCA) treatment. Characterization of the purified EPS was carried out with respect to fourier-transform infrared (FTIR) spectrum, 1 H nuclear magnetic resonance (NMR) spectrum and mass spectrometry (MS) analysis.Results: Two-fold increase in the yield of EPS (23 g/l) by the selected isolate was obtained by using culture conditions as 10% inoculum size having cell density of 10 7 cells/ml, pH 6, incubation temperature 45 °C, 3% carbohydrate, 0.5% yeast extract as nitrogen source, 20% salt concentration and 7 d of incubation period. Characterization of the purified EPS suggested the presence of dominated glycosidic linkages and heptasaccharide nature of the molecule. As the present strain is halophilic, 20% NaCl was found to be optimum. Conclusion:Optimization studies resulted in two-fold increase in the yield of EPS which is of heptasaccharide nature.

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Halomonas alkaliantarctica sp. nov., isolated from saline lake Cape Russell in Antarctica, an alkalophilic moderately halophilic, exopolysaccharide-producing bacterium

Cited by 100 publications

References 31 publications

Diversity and activity of cultivable aerobic planktonic bacteria of a saline Lake located in Sovata, Romania

Diversity and activity of cultivable aerobic planktonic bacteria of a saline Lake located in Sovata, Romania

Bacterial Polymers Produced by Extremophiles: Biosynthesis, Characterization, and Applications of Exopolysaccharides

Production and Characterization of Exopolysaccharide From Marine Moderately Halophilic Bacterium Halomonas Smyrnensis SVD Iii

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