Halorubrum californiense sp. nov., an extreme archaeal halophile isolated from a crystallizer pond at a solar salt plant in California, USA

Pesenti, Peter T.; Sikaroodi, Masoumeh; Gillevet, Patrick M.; Sánchez‐Porro, Cristina; Ventosa, António; Litchfield, Carol D.

doi:10.1099/ijs.0.2008/002410-0

Cited by 38 publications

(17 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DNA G+C contents of strains RO2-11 T and HO2-1 are 55.7 and 57.6 mol%, respectively. These values are lower than those of the genera Halorubrum (61.9-71.2 mol%; Pesenti et al, 2008), Halosarcina (65.4 mol%; Savage et al, 2008), Haloplanus (66.2 mol%; Elevi Bardavid et al, 2007) and Haloferax (59.5-65.3 mol%;Allen et al, 2008) and of Halogeometricum borinquense (59.1 mol%; Montalvo-Rodriguez et al, 1998), but higher than the value reported for Haloquadratum walsbyi (46.9 mol%; Burns et al, 2007). The DNA relatedness between strains RO2-11 T and HO2-1 was 89.2 %.…”

mentioning

confidence: 54%

“…S2). The absence of phosphatidylglycerol sulfate (PGS) within this group helps to differentiate the two isolates from neutrophilic members of the genera Halorubrum, Haloplanus and Haladaptatus, which contain PGS (Pesenti et al, 2008;Bardavid et al, 2007;Savage et al, 2007). The glycolipid profile sets strains RO2-11 T and HO2-1 apart from members of the genera Haloferax and Halosarcina, which possess S-DGD-1 and DGD-1 (Allen et al, 2008;Savage et al, 2008), and the member of the genus Haloquadratum, which only contains S-DGD-1 (Burns et al, 2007).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Halogranum rubrum gen. nov., sp. nov., a halophilic archaeon isolated from a marine solar saltern

Cui

Gao

Sun

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two extremely halophilic archaea, strains RO2-11(T) and HO2-1, were isolated from two Chinese marine solar salterns, Rudong solar saltern and Haimen solar saltern, respectively. Cells of the two strains were polymorphic and Gram-stain-negative; colonies were red-pigmented. The two strains grew at NaCl concentrations of 2.6-4.3 M (optimum 3.9 M) and required at least 0.1 M Mg2+ for growth. They were able to grow over a pH range of 6.0-8.0 and a temperature range of 20-50 degrees C, with optimal pH of 7.5 and optimal temperature of 37 degrees C. The major polar lipids of strain RO2-11(T) and strain HO2-1 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and three glycolipids, two of them chromatographically identical to S-DGD-1 and DGD-1, the third unidentified. The 16S rRNA gene sequence similarity of strain RO2-11(T) and strain HO2-1 was 99.3 % and highest sequence similarity with the closest relative (Haloferax larsenii) was 91.4 %. Based on the data obtained, the two isolates could not be classified in any recognized genus of the family Halobacteriaceae. Strain RO2-11(T) and strain HO2-1 are thus considered to represent a novel species of a new genus within the family Halobacteriaceae, for which the name Halogranum rubrum gen. nov., sp. nov. is proposed. The type strain is RO2-11(T) (=CGMCC 1.7738(T) =JCM 15772(T)).

show abstract

mentioning

confidence: 54%

mentioning

confidence: 99%

Halogranum rubrum gen. nov., sp. nov., a halophilic archaeon isolated from a marine solar saltern

Cui

Gao

Sun

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…In all cases, most of the retrieved species of this genus were related to Hrr. californiense, which was originally described from a crystallizer pond at the Cargill Solar Salt Plant in California [39]. This species was especially relevant in numbers in the Mediterranean and Atlantic sites, although it was present in all samples (Fig.…”

Section: Discussionmentioning

confidence: 98%

Diversity of extremely halophilic cultivable prokaryotes in Mediterranean, Atlantic and Pacific solar salterns: Evidence that unexplored sites constitute sources of cultivable novelty

Viver

Cifuentes

Díaz

et al. 2015

Systematic and Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…The major polar lipids are C 20 C 20 and sometimes C 20 C 25 Modern classification of the haloarchaea requires using phenotypic characterization, a chemotaxonomic approach based on the polar lipid composition as well as comparison of the 16S rRNA gene sequence, which is considered a universal phylogenetic and taxonomic molecular marker (Oren, 2012). The usefulness of this molecular marker, however, is questionable for haloarchaeal taxonomic studies as, over the years, studies have demonstrated that it has many qualities that make it difficult to distinguish species, such as: recombination between genera (Boucher et al, 2004;Papke et al, 2015) and closely related species (Papke et al, 2004(Papke et al, , 2007; that high sequence conservation does not discriminate between closely related species (Pesenti et al, 2008;Mancinelli et al, 2009); that their rRNA operons undergo intragenic recombination (Boucher et al, 2004); and that many genera (e.g. Haloarcula, Halosimplex and Halomicrobium) contain multiple gene copies with more than 5 % sequence divergence (Boucher et al, 2004).…”

Section: Two Extremely Halophilic Archaea Strains Cb34mentioning

confidence: 99%

Halorubrum halodurans sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake

Corral

Haba

Sánchez‐Porro

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two extremely halophilic archaea, strains Cb34T and C170, belonging to the genus Halorubrum, were isolated from the brine of the hypersaline lake Aran-Bidgol in Iran. Cells of the two strains were motile, pleomorphic rods, stained Gram-variable and produced redpigmented colonies. Strains Cb34 T and C170 required 25 % (w/v) salts, pH 7.0 and 37 8C for optimal growth under aerobic conditions; 0.3 M Mg 2+ was required. Cells of both isolates were lysed in distilled water and hypotonic treatment with ,10 % NaCl provoked cell lysis. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that these two strains were closely related to Halorubrum cibi B31 T (98.8 %) and other members of the genusHalorubrum. In addition, studies based on the rpoB9 gene revealed that strains Cb34 T and C170 are placed among the species of Halorubrum and are closely related to Halorubrum cibi B31 T , with rpoB9 gene sequence similarity less than or equal to 95.7 %. The polar lipid patterns of both strains consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The DNA G+C content was 62.1-62.4 mol%. DNA-DNA hybridization studies confirmed that strains Cb34 T and C170 constitute a distinct species. Data obtained in this study show that the two strains represent a novel species, for which the name Halorubrum halodurans sp. nov. is proposed. The type strain is Cb34 T (5CECT 8745 T 5IBRC-M 10233 T ).The haloarchaea are a group of extremely halophilic, aerobic archaea included in the class Halobacteria ) that comprises 48 genera including the genus Halorubrum, established by McGenity & Grant (1995). Currently, it is the largest haloarchaeal genus, with 31 validly published species names (Parte, 2015). Species of the genus Halorubrum have been isolated from diverse natural and artificial hypersaline environments such as salterns, salt lakes, coastal sabkhas, soda lakes, saline soils and salt-fermented and salt-preserved food. It is often reported as the dominant genus present in many hypersaline environments; their ubiquity and adaptability to nearly all possible hypersaline conditions has been demonstrated by cultivation and culture-independent methodologies (Ghai et al., 2011; Makhdoumi-Kakhki et al., 2012a; Fernández et al., 2014a, b). Species of this genus are aerobic, chemo-organotrophic and require high concentrations of NaCl in media (e.g. 1.0-5.2 M) for growth. The genus is phenotypically highly variable: its members use many carbon substrates including a wide range of sugars as sources of energy. However, some species use only single carbon sources for growth. They produce bacterioruberin carotenoid pigments that colour colonies pink to red. Most described species are neutrophilic, but some are alkaliphilic and grow optimally at pH 9.5. The major polar lipids are C 20 C 20 and sometimes C 20 C 25 Modern classification of the haloarchaea requires using phenotypic characterization, a chemotaxonomic approach based on the polar l...

show abstract

Halorubrum californiense sp. nov., an extreme archaeal halophile isolated from a crystallizer pond at a solar salt plant in California, USA

Cited by 38 publications

References 44 publications

Halogranum rubrum gen. nov., sp. nov., a halophilic archaeon isolated from a marine solar saltern

Halogranum rubrum gen. nov., sp. nov., a halophilic archaeon isolated from a marine solar saltern

Diversity of extremely halophilic cultivable prokaryotes in Mediterranean, Atlantic and Pacific solar salterns: Evidence that unexplored sites constitute sources of cultivable novelty

Halorubrum halodurans sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake

Contact Info

Product

Resources

About