Haltbarkeitsspezifische dünnschichtchromatographische Bestimmungen des Morphins und seiner Abbauprodukte

Ebel, S.; Rost, Dieter

doi:10.1002/ardp.19803130410

Cited by 7 publications

(2 citation statements)

References 16 publications

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“…Several analytical methods have been reported for the determination of these important opium alkaloids. The analytical techniques include paper chromatography [2], thin-layer chromatography [3], UV spectroscopy [4], gas chromatography (GC) [5], liquid chromatography (LC) [6], and radio-immunoassay [7]. Many of these techniques have limitations with respect to sensitivity (TLC), matrix interference (UV), derivatization (GC), and poorly resolved asymmetrical peaks (HPLC) [8].…”

Section: Introductionmentioning

confidence: 99%

Application of capillary zone electrophoresis in the separation and determination of the principal gum opium alkaloids

et al. 2003

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We describe the use of capillary zone electrophoresis (CZE) for the qualitative and quantitative determination of major alkaloids (i.e., thebaine, codeine, morphine, papavarine and narcotine) in gum opium involving the analysis of alkaloids without derivatization or purification. Three extractions with 2.5% w/v aqueous acetic acid quantitatively extracted major alkaloids. The separation was carried out by CZE using a 7:3 mixture of methanol and sodium acetate (100 mM, pH 3.1) at a potential of 15 kV, with UV detection at 224 nm. Spiking of pure reference alkaloid standards in the opium extract was used for peak identification. The influences of buffer composition, pH and voltage on the separation of alkaloids were studied. The detection limit of each alkaloid dissolved in methanol was found to be 850 ng/mL (morphine), 450 ng/mL (thebaine), 500 ng/mL (codeine), 550 ng/mL (papaverine), and 500 ng/mL (narcotine) at an injection pressure of 300 mbar (injection volume, 4 nL) with a signal-to-noise ratio of 3:1. The external standard method was used for the quantification of alkaloids. The calibration plot was based on linear regression analysis. The relative standard deviation (RSD) for peak area and migration time was in the range of 1.03-3.56% and 0.34-0.69%, respectively. Percentage compositions (g%) of opium alkaloids in five gum opium samples were found to be in the range of 14.45-15.95 (morphine), 2.0-3.45 (codeine), 1.32-2.73 (thebaine), 0.92-2.37 (papavarine), and 3.85-5.77 (narcotine). The method developed is suitable for the routine analysis of major gum opium alkaloids in samples of forensic importance.

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Section: Introductionmentioning

confidence: 99%

Application of capillary zone electrophoresis in the separation and determination of the principal gum opium alkaloids

et al. 2003

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“…Up to now, various analytical methods for the determination of morphine in poppy shells have been developed, containing ultraviolet spectrophotometry, 4 thin layer chromatography (TLC), 5 high performance liquid chromatography (HPLC), 6 gas chromatography (GC), 7 capillary electrophoresis (CE) 8 and mass spectrometry (MS). 9–13 UV and TLC lack sensitivity and specificity for the determination of morphine in poppy shells.…”

Section: Introductionmentioning

confidence: 99%

Novel N,Cl-doped deep eutectic solvents-based carbon dots as a selective fluorescent probe for determination of morphine in food

et al. 2021

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Voltammetric Oxidation of Drugs of Abuse I. Morphine and Metabolites

Garrido¹,

Delerue–Matos²,

Borges

et al. 2004

Electroanalysis

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A detailed study of the electrochemical oxidative behavior of morphine in aqueous solution is reported. Through the synthesis of several metabolites and derivatives, pseudomorphine, morphine N-oxide, normorphine, dihydromorphine and 2-(N,N-dimethylaminomethyl)morphine, and their voltammetric study it was possible to identify the oxidation peaks for morphine. The anodic waves are related with the oxidation of phenolic and tertiary amine groups. It is also possible to verify that a poorly defined peak observable during morphine oxidation is not a consequence of further oxidation of pseudomorphine but due to formation of a dimer during phenolic group oxidation. The results obtained and especially those regarding the formation of a new polymer based on a CÀO coupling could be useful for clarifying the discoloration phenomenon occurring during storage of morphine solutions as well as leading to a better understanding of its oxidative metabolic pathways.

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Haltbarkeitsspezifische dünnschichtchromatographische Bestimmungen des Morphins und seiner Abbauprodukte

Cited by 7 publications

References 16 publications

Application of capillary zone electrophoresis in the separation and determination of the principal gum opium alkaloids

Application of capillary zone electrophoresis in the separation and determination of the principal gum opium alkaloids

Novel N,Cl-doped deep eutectic solvents-based carbon dots as a selective fluorescent probe for determination of morphine in food

Voltammetric Oxidation of Drugs of Abuse I. Morphine and Metabolites

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