Handbook of Cell Biosensors

doi:10.1007/978-3-319-47405-2

Cited by 11 publications

(1 citation statement)

References 95 publications

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“…In the present study, we estimated the effects of pesticide formulations using an alternative analysis procedure, based on the in vivo bacterial bioluminescence assay, which had been proven effective in ecotoxicology monitoring [46][47][48]. As toxicants induce the disruption of cellular metabolism and, hence, a quick decrease in light emission, the in vivo method based on P. phosphoreum is a simple and convenient screening assay [46,49]. However, the results of the present study considered as a whole demonstrate that the sensitivity of in vitro and in vivo assay systems to exposure to commercial pesticide formulations increased as follows: the assay based on P. phosphoreum < single-enzyme reactions < multi-enzyme reactions.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Commercial Pesticide Formulations on the Function of In Vitro and In Vivo Assay Systems: A Comparative Analysis

et al. 2022

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Pesticides are commonly used in agriculture and are an important factor of food security for humankind. However, the overuse of pesticides can harm non-target organisms, and, thus, it is vital to comprehensively study their effects on the different metabolic pathways of living organisms. In the present study, enzyme-inhibition-based assays have been used to investigate the effects of commercial pesticide formulations on the key enzymes of the organisms, which catalyze a wide variety of metabolic reactions (protein catabolism, lactic acid fermentation, alcohol metabolism, the conduction of nerve impulses, etc.). Assay conditions have been optimized, and the limitations of the methods used in the study, which are related to the choice of the solvent for commercial pesticide formulations and optical effects occurring when commercial pesticide formulations are mixed with solutions of enzymes and substrates of assay systems, have been revealed. The effects of commercial pesticide formulations on simple chemoenzymatic assay systems (single-enzyme reactions) have been compared to their effects on complex multicomponent molecular systems (multi-enzyme reactions) and organisms (luminescent bacterium). The in vitro assay systems have shown higher sensitivity to pesticide exposure than the in vivo assay system. The sensitivity of the in vitro assay systems increases with the elongation of the chain of conjugated chemoenzymatic reactions. The effects exerted by commercial pesticide formulations with the same active ingredient but produced by different manufacturers on assay system functions have been found to differ from each other.

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Section: Discussionmentioning

confidence: 99%

The Effects of Commercial Pesticide Formulations on the Function of In Vitro and In Vivo Assay Systems: A Comparative Analysis

et al. 2022

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Plasmonic biosensor for the study of blood diseases by analysis of hemoglobin concentration

2023

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Plasmonic materials and phenomena are widely studied and applied in multiple fields for an extended time. One among the foremost promising applications is within the engineering of biosensor devices, Diseases of the red blood cells are among the diseases of the blood. Anemia as an example is an abnormal reduction within the concentration of hemoglobin within the blood. When the amount of red blood cells decreases, so does the concentration of hemoglobin. The tissues and organs not receive sufficient oxygen to work normally. This numerical study will give a contribution or approach on the behavior of blood with surface plasmons resonance SPR by analyzing the performance of SPR with any changes in hemoglobin concentrations (refractive index) for the application of technique in the detection of blood diseases.

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A general strategy to engineer high-performance mammalian Whole-Cell Biosensors

Mallozzi,

Fusco,

Ragazzini

et al. 2024

Preprint

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Transcription-based whole-cell biosensors (WCBs) are cells engineered with an analyte-responsive promoter driving the transcription of a reporter gene. WCBs can sense and report on bioactive molecules (analytes) relevant to human health. Designing an analyte-sensitive promoter requires a cumbersome trial-and-error approach and usually results in biosensors with poor performance. Here, we integrated Synthetic Biology with Control Engineering to design, computationally model, and experimentally implement high-performance biosensors in mammalian cells. Our approach, unlike traditional methods, does not rely on optimizing individual components such as promoters and transcription factors. Instead, it uses biomolecular circuits to enhance the biosensor’s performance despite inherent component flaws. We experimentally implemented eight different biosensors by employing CRISPR-Cas systems, then quantitatively compared their performance and identified one configuration, which we named CASense, that overcomes the limitations of current biosensors. Our approach is generalisable and can be adapted to sense any analyte of interest for which there is an analyte-sensitive promoter, making it a versatile tool for multiple applications. As a proof of concept, we engineered a high-performance biosensor of intracellular copper due to the critical role that copper plays in human health and disease, and to the lack of techniques able to measure intracellular copper levels in living cells. The significance of our work lies in its potential to impact the monitoring of bioactive molecules and chemicals both in vitro and in vivo, which is crucial in areas such as toxicology, drug discovery, disease diagnosis and therapy.

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Handbook of Cell Biosensors

Cited by 11 publications

References 95 publications

The Effects of Commercial Pesticide Formulations on the Function of In Vitro and In Vivo Assay Systems: A Comparative Analysis

The Effects of Commercial Pesticide Formulations on the Function of In Vitro and In Vivo Assay Systems: A Comparative Analysis

Plasmonic biosensor for the study of blood diseases by analysis of hemoglobin concentration

A general strategy to engineer high-performance mammalian Whole-Cell Biosensors

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