Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis

Echlin, Patrick

doi:10.1007/978-0-387-85731-2

Cited by 182 publications

(150 citation statements)

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“…Samples from the kidneys of fetuses at E19, E25, and E30 and from rabbit aged one and two weeks and two months were used for SEM examination according to Echlin (2009). The samples were immediately immersed in 4F1G (2% formaldehyde, 1.25%glutaraldehyde in 0.1 M sodium cacodylate buffer) fixative at pH 7.2 and stored at 4°C.…”

Section: Scanning Electron Microscopic (Sem) Examinationmentioning

confidence: 99%

Morphogenesis of Rabbit Kidney Pre-and Postnatal

Fayez¹,

Sayed-Ahmed²,

Abo-Ghanema³

et al. 2014

AJVS

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Key words ABSTRACT:rabbit, kidney, morphogenesis, mesonephros, metanephrosMammalian renal development differs from that of most of the other organs in that it proceeds through a series of three successive phases, each is marked by the formation of a more complex pair of kidneys. These kidneys are called the pronephros, mesonephros, and metanephros. The current study has been conducted to elucidate the developmental changes of rabbit kidneys during their pre-and postnatal life. In the present study we used samples, at different ages, of the rabbit kidneys from embryonic day 15 (E15) till maturity for light and scanning electron microscopical investigation. At E15, large mesonephros, occupied a great part of abdominal cavity of rabbit embryo, and undifferentiated metanephros were noticed. At E19 metanephros became differentiated into cortex and medulla with the initiation of nephron-forming stage. Additionally at this stage, the caudal series of mesonephros still detected but in degenerated and atrophied structure. Just before birth, at E30, the kidney demonstrated well-developed renal corpuscles, differentiated proximal and distal convoluted tubules, with apparently detectable loop of Henle and large collecting ducts. Shortly after birth rabbit kidney showed further morphogenesis and at two-months old, the kidney of rabbit with mature histological structure of the renal parenchyma was documented. In conclusion, the development of rabbit kidneys occurred mainly during the prenatal period while their histological maturity occurred from two to three weeks after birth. The present findings were discussed with previous publications.

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Section: Scanning Electron Microscopic (Sem) Examinationmentioning

confidence: 99%

Morphogenesis of Rabbit Kidney Pre-and Postnatal

Fayez¹,

Sayed-Ahmed²,

Abo-Ghanema³

et al. 2014

AJVS

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“…This surface is adequate for SEM observation and analysis (Fig. 2b) [31]. The backscattering image (Fig.…”

Section: Artwork Sample Analysismentioning

confidence: 92%

Optimal Sample Preparation for the Analysis of Micrometric Heterogeneous Samples

et al. 2015

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Precise microanalytical techniques are essential in many fields such as Cultural Heritage materials, showing complex layered microstructures containing a wide range of materials of diverse nature and hardness. Non-invasive sample manipulation and preparation is required to avoid, as far as possible, sample contamination which may strongly limit the materials identification.The method proposed consists in the application of thin gold or carbon protecting layers before embedding the samples in synthetic resin for microtoming. The validity and optimal procedure is checked for those materials most often found on the paintings surface: varnishes (natural resins and wax).An artwork sample is similarly prepared and analysed by Optical Microscopy (OM), Scanning Electron Microscopy (SEM/EDS), Micro Infrared Spectroscopy (µFTIR/ µSR-FTIR) and X-Ray diffraction (µSR-XRD) with synchrotron light.

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“…The samples were mounted on specimen stub using electrically conductive double sided adhesive tape and sputter coated with gold before examination in electron microscope. 16 …”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Effect of Propranolol; Rifampicin Induced Hepatotoxicity in Rabbit’s Liver

Abrar

Naqvi

Ahmed

et al. 2017

TPMJ

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Tuberculosis is the disease required long term treatment. Rifampicin is the major element of antiTB therapy if resistance is not documented. Potential of antitubercular dugs to produced hepatoxicity is very high and among all antiTB agents rifampicin (RIF) induced hepatoxicity stands on top. Rifampicin is the major element of antiTB therapy if resistance is not documented. But its hepatotoxic effects are the main hurdle to continue with this therapy. In this study RIF were administered to the rabbit alone or in combination of propranolol to evaluate the hepatotoxic effects of RIF and reduction of hepatotoxicity by propranolol. Histological evaluation of liver tissue on higher magnification, its micrometric analysis and SEM (scanning electron microscopy) of liver were used to estimate the effects of this combination. Micrometry revealed that number of viable hepatocytes, their diameter and nuclear diameter were altered. SEM micrograph showed distorted and swollen hepatic cords. All of these changes successfully turned to normal by combined administration of propranolol. Propranolol successfully improves the hepatic architecture proved by both qualitative and quantitative microscopy. Key words:Hepatotoxicity, antitubercular therapy, micrometric analysis, scanning electron microscopy, hepatic cords, hepatocytes.

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Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis

Cited by 182 publications

References 0 publications

Morphogenesis of Rabbit Kidney Pre-and Postnatal

Morphogenesis of Rabbit Kidney Pre-and Postnatal

Optimal Sample Preparation for the Analysis of Micrometric Heterogeneous Samples

Effect of Propranolol; Rifampicin Induced Hepatotoxicity in Rabbit’s Liver

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