Handheld and Cost-Effective Fourier Lightfield Microscope

Abstract

In this work, the design, building, and testing of the most portable, easy-to-build, robust, handheld, and cost-effective Fourier Lightfield Microscope (FLMic) to date is reported. The FLMic is built by means of a surveillance camera lens and additional off-the-shelf optical elements, resulting in a cost-effective FLMic exhibiting all the regular sought features in lightfield microscopy, such as refocusing and gathering 3D information of samples by means of a single-shot approach. The proposed FLMic features reduced dimensions and light weight, which, combined with its low cost, turn the presented FLMic into a strong candidate for in-field application where 3D imaging capabilities are pursued. The use of cost-effective optical elements has a relatively low impact on the optical performance, regarding the figures dictated by the theory, while its price can be at least 100 times lower than that of a regular FLMic. The system operability is tested in both bright-field and fluorescent modes by imaging a resolution target, a honeybee wing, and a knot of dyed cotton fibers.

Machine Learning-Based View Synthesis in Fourier Lightfield Microscopy

“…The aim of this paper is to show the utility of NeRF--in Fourier lightfield microscopy (FLMic) [16][17][18][19][20][21], which is a promising technique for the rapid acquisition of 3D information of microscopic samples. In fact, this system is able to acquire angular and spatial information of the sample in a single shot.…”