Handling and Sensing of Single Enzyme Molecules: From Fluorescence Detection towards Nanoscale Electrical Measurements

Mathwig, Klaus; Chi, Qijin; Lemay, Serge G.; Rassaei, Liza

doi:10.1002/cphc.201500686

Cited by 14 publications

(14 citation statements)

References 56 publications

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“…The interest to consider single enzyme molecules is to provide a distribution of conformational heterogeneities, and reaction mechanisms that are not observed otherwise due to averaging. However, SM or SP imaging remains a challenge . In most studies described here, enzyme was in direct electronic contact with the electrode.…”

Section: Mechanism Structure Motion Conformationmentioning

confidence: 99%

See 1 more Smart Citation

Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

2019

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Redox enzymes, which catalyze electron transfer reactions in living organisms, can be used as selective and sensitive bioreceptors in biosensors, or as efficient catalysts in biofuel cells. In these bioelectrochemical devices, the enzymes are immobilized at a conductive surface, the electrode, with which they must be able to exchange electrons. Different physicochemical methods have been coupled to electrochemistry to characterize the enzyme‐modified electrochemical interface. In this Review, we summarize most efforts performed to investigate the enzymatic electrodes at the micro‐ and even nanoscale, thanks to microscopy techniques. Contrary to electrochemistry, which gives only a global information about all processes occurring at the electrode surface, microscopy offers a spatial resolution. Several techniques have been implemented; mostly scanning probe microscopies like atomic force microscopy, scanning tunneling microscopy, and scanning electrochemical microscopy, but also scanning electron microscopy and fluorescence microscopy. These studies demonstrate that various information can be obtained thanks to microscopy at different scales. Electrode imaging has been performed to confirm the presence of enzymes, to quantify and localize the biomolecules, but also to evaluate the morphology of immobilized enzymes, their possible conformation changes upon turnover, and their orientation at the electrode surface. Local redox activity has also been imaged and kinetics has been resolved.

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Section: Mechanism Structure Motion Conformationmentioning

confidence: 99%

“…and not immobilized at a surface. We invite the interested reader to have a look at a review by Lemay and co‐workers . To the best of our knowledge, very few studies have been realized in an electrochemical context.…”

Section: Kineticsmentioning

confidence: 99%

Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

2019

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“…It is anticipated that further improvement of this approach would lead to electrochemical study of enzyme kinetics at a single-molecule level. 98 …”

Section: New Applications Of Nanoelectrodesmentioning

confidence: 99%

Recent advances in the development and application of nanoelectrodes

Fan

Han

Zhang

2016

Analyst

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Nanoelectrodes have key advantages compared to electrodes of conventional size and are the tool of choice for numerous applications in both fundamental electrochemistry research and bioelectrochemical analysis. This Minireview summarizes recent advances in the development, characterization, and use of nanoelectrodes in nanoscale electroanalytical chemistry. Methods of nanoelectrode preparation include laser-pulled glass-sealed metal nanoelectrodes, mass-produced nanoelectrodes, carbon nanotube based and carbon-filled nanopipettes, and tunneling nanoelectrodes. Several new topics of recent application are covered, which include the use of nanoelectrodes for electrochemical imaging at ultrahigh spatial resolution, imaging with nanoelectrodes and nanopipettes, electrochemical analysis of single cells, single enzymes, and single nanoparticles, and the use of nanoelectrodes to understand single nanobubbles.

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“…Nanofluidic devices are important in sensing, in energy conversion, and in scanning electrochemical microscopies (SECMs) . With an applied potential across the nanofluidic device, ionic rectifier or “ionic diode” effects are observed when charge transport occurs asymmetrically, i. e., when a component of the nanofluidic device introduces asymmetry .…”

Section: Introductionmentioning

confidence: 99%

Ionic Transport in Microhole Fluidic Diodes Based on Asymmetric Ionomer Film Deposits

2017

Self Cite

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Microhole fluidic ionic diodes based on asymmetric deposits of charged ionomer membranes (e. g. Nafion or polymers of intrinsic microporosity) on microhole supports yield high rectification ratios for ionic transport. They are fabricated without the need for complex micro‐ or nanostructuring, and show potential for future applications in desalination and biosensing. Here, we propose an explanation for the functional principle for this type of materials‐based ionic diode. A predictive computational model for ionic diode switching is based on finite element analysis. It is employed to determine the influence of diode geometry as well as type and concentration of aqueous electrolyte on the rectification behavior.

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Handling and Sensing of Single Enzyme Molecules: From Fluorescence Detection towards Nanoscale Electrical Measurements

Cited by 14 publications

References 56 publications

Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

Recent advances in the development and application of nanoelectrodes

Ionic Transport in Microhole Fluidic Diodes Based on Asymmetric Ionomer Film Deposits

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