Hantaviruses in Finnish soricomorphs: Evidence for two distinct hantaviruses carried by Sorex araneus suggesting ancient host-switch

Ling, Jiaxin; Sironen, Tarja; Voutilainen, Liina; Hepojoki, Satu; Niemimaa, Jukka; Isoviita, Veli‐Matti; Vaheri, Antti; Henttonen, Heikki; Vapalahti, Olli

doi:10.1016/j.meegid.2014.06.023

Cited by 24 publications

(37 citation statements)

References 42 publications

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“…The polyclonal antibodies for PUUV and SWSV have been described previously (Ling et al, 2014;Vapalahti et al, 1995b). transmitted to man (i.e.…”

Section: Resultsmentioning

confidence: 99%

“…To our knowledge cell culture isolates of SWSV do not exist, which to some extent compromises the use of the term virus; however, for the sake of clarity we will use SWSV when referring to Seewis virus. Soon after its discovery, SWSV was also detected in Austria, the Czech Republic, Finland, Germany, Hungary, Poland, Russia, Slovakia and Slovenia (Kang et al, 2009;Ling et al, 2014;Resman et al, 2013;Schlegel et al, 2012b;Song et al, 2007b;Yashina et al, 2010). SWSV is divided into three genotypes; the Finnish subtype is distant from both the Russian and Central European SWSV subtypes.…”

Section: Introductionmentioning

confidence: 99%

“…SWSV shares its most recent common ancestor with the Muridae-associated hantaviruses HTNV and SEOV, which is considered to be due to a horizontal transmission event between the rodents and soricomorphs (Plyusnin & Sironen, 2014). The prevalence of SWSV is fairly high and about 12 to 14 % of common shrews are positive for SWSV RNA (Ling et al, 2014;Schlegel et al, 2012b). SWSV is geographically widespread but the evidence for SWSV existence is limited to nucleic acid detection and serological evidence is lacking.…”

Section: Introductionmentioning

confidence: 99%

“…The production of polyclonal rabbit antisera against the N-terminal (1-120 aa) fragment of SWSV N protein and the N-terminal fragment (1-267 aa) of the PUUV N protein were described previously (Ling et al, 2014;Vapalahti et al, 1995b). The mammalian expression plasmids for TULV and PUUV N, pcDNA3.1(+)-TULVN and pcDNA3.1(+)-PUUVN, were constructed elsewhere (Kaukinen et al, 2003;Li et al, 2002).…”

mentioning

confidence: 99%

“…The insert was verified by DNA sequencing. Shrew heart extracts from an earlier study (Ling et al, 2014) were used to characterize the recombinant SWSV N protein produced in insect cells. Recombinant antigens, SWSV and PUUV N protein. Purified recombinant PUUV N protein described by Vapalahti et al (1995b) was obtained from Reagena.…”

mentioning

confidence: 99%

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Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Ling

Vaheri

Hepojoki

et al. 2015

Journal of General Virology

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Puumala virus (PUUV, carried by Myodes glareolus) co-circulates with Seewis virus (SWSV, carried by Sorex araneus) in Finland. While PUUV causes 1000-3000 nephropathia epidemica (NE) cases annually, the pathogenicity of SWSV to man is unknown. To study the prevalence of SWSV antibodies in hantavirus fever-like patients' sera, we used recombinant SWSV nucleocapsid (N) protein as the antigen in ELISA, immunofluorescence assay (IFA) and immunoblotting. While characterizing the recombinant SWSV N protein, we observed that a polyclonal rabbit antiserum against PUUV N protein cross-reacted with SWSV N protein and vice versa. We initially screened 486 (450 PUUV-seronegative and 36 PUUV-seropositive) samples sent to Helsinki University Hospital Laboratory for PUUV serodiagnosis during 2002 and 2007 in an SWSV N protein IgG ELISA. In total, 4.2 % (19/450) of the PUUVseronegative samples were reactive in the SWSV N protein IgG ELISA and none of the tested samples [43 PUUV-seronegative (weakly reactive in the SWSV IgG ELISA) and 15 random] were reactive in the SWSV N protein IgM ELISA. None of the IgG reactions could be confirmed by IFA or immunoblotting. Furthermore, among the 36 PUUV-seropositive samples three were reactive in SWSV N protein IgG and ten in SWSV N protein IgM ELISA. One PUUVseropositive sample reacted with SWSV N protein in IFA and four in immunoblotting. Finally, we applied competitive ELISA to confirm that the observed reactivity was due to cross-reactivity rather than a true SWSV response. In conclusion, no evidence of SWSV infection was found among the 486 samples studied; however, we did demonstrate that PUUV antiserum cross-reacted with shrew-borne hantavirus N protein.

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“…The polyclonal antibodies for PUUV and SWSV have been described previously (Ling et al, 2014;Vapalahti et al, 1995b). transmitted to man (i.e.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Ling

Vaheri

Hepojoki

et al. 2015

Journal of General Virology

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Genetic analyses of Seoul hantavirus genome recovered from rats (Rattus norvegicus) in the Netherlands unveils diverse routes of spread into Europe

Ling

Verner-Carlsson

Eriksson

et al. 2019

Journal of Medical Virology

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Seoul virus (SEOV) is the etiologic agent of hemorrhagic fever with renal syndrome. It is carried by brown rats (Rattus norvegicus), a commensal rodent that closely cohabitates with humans in urban environments. SEOV has a worldwide distribution, and in Europe, it has been found in rats in UK, France, Sweden, and Belgium, and human cases of SEOV infection have been reported in Germany, UK, France, and Belgium. In the search of hantaviruses in brown rats from the Netherlands, we found both serological and genetic evidence for the presence of SEOV in the local wild rat population. To further decipher the relationship with other SEOV variants globally, the complete genome of SEOV in the Netherlands was recovered. SEOV sequences obtained from three positive rats (captured at close trapping locations at the same time) were found highly similar. Phylogenetic analyses demonstrated that two lineages of SEOV circulate in Europe. Strains from the Netherlands and UK, together with the Baxter strain from US, constitute one of these two, while the second includes strains from Europe and Asia. Our results support a hypothesis of diverse routes of SEOV spread into Europe. These findings, combined with other indications on the expansion of the spatial European range of SEOV, suggest an increased risk of this virus for the public health, highlighting the need for increased surveillance.

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Hantavirus: General Features and Present Situation in Latin America

Delfraro

Raboni

Santos

2017

Human Virology in Latin America

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Hantaviruses in Finnish soricomorphs: Evidence for two distinct hantaviruses carried by Sorex araneus suggesting ancient host-switch

Cited by 24 publications

References 42 publications

Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

Genetic analyses of Seoul hantavirus genome recovered from rats (Rattus norvegicus) in the Netherlands unveils diverse routes of spread into Europe

Hantavirus: General Features and Present Situation in Latin America

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