Haplo-insufficiency for different genes differentially reduces pathogenicity and virulence in a fungal phytopathogen

Pham, Cau D.; Yu, Zhanyang; Lovely, C. Ben; Agarwal, Charu; Myers, David A.; Paul, Jinny; Cooper, Marie; Barati, Michelle T.; Perlin, Michael H.

doi:10.1016/j.fgb.2011.11.007

Cited by 5 publications

(7 citation statements)

References 48 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the d132Δptn1 strain showed reduced formation of aerial hyphae (Figure 2.3 B), compared to wild type. This result indicates that ptn1 gene does show some level of haplo-insufficiency in the d132 diploid background, as has been observed for other genes in this species (Pham et al, 2012) . In contrast, the loss of ptn1 appears to be at least partially compensated by the presence of the wild type allele in dikaryons as demonstrated by 2/9 x 1/2 Δptn1.…”

Section: Figure 23 (A)supporting

confidence: 80%

“…A similar type of morphology was described during the overexpression of smu1 in hsl1 deletion mutants (Lovely et al, 2011) and these genes are demonstrated to have a role in cell separation and cytokinesis in S. cerevisiae (Shulewitz, Inouye, & Thorner, 1999). It is known that when a single copy of pdc1 is deleted as in d132 Δpdc1, there were no effects on the aerial hyphal formation (Pham et al, 2012). However, the fact that Pdc1 physically interacts with Ptn1 led us to explore the effect of double deletion of pdc1 and ptn1.…”

Section: Resultsmentioning

confidence: 63%

“…On the other hand, the deletion of one copy of rho1 in a diploid strain results in the loss of ability of the fungus to form aerial filaments on charcoal plates (Pham et al, 2012). To B DI=2.23 DI= 1.04 DI=1.66 DI=0.8 * explore the possible connection between ptn1 and rho1, we attempted to delete ptn1 in diploid d132 strains where one copy of rho1 was deleted.…”

Section: Resultsmentioning

confidence: 99%

“…Plant infection was performed by inoculating 7-9 days old maize seedlings [Golden Bantam seeds, Bunton Seed Co., Louisville, KY and W. Atlee Burpee & Co., 62 Warminster, PA]. Haploid strains of opposite mating types were mixed prior to plant infection, while diploid d132 and the solopathogenic SG200 strain were used directly as previously described (Gold et al, 1997;Pham et al, 2012). The virulence of each treatment group was scored by a disease index (DI) on a scale of 0 to 5, where 0= No Symptoms, 1= Chlorosis, 2= Small leaf tumors, 3=…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Exploring the role of PTN1 in ustilago maydis.

Pillai¹,

Krishna²

View full text Add to dashboard Cite

Signal transduction is a key aspect of biological life. PTEN (phosphatase and tensin homolog) is a widely studied signaling protein in mammalian systems, where it acts as a tumor suppressor. Numerous studies have conclusively demonstrated the anti-cancerous effect of this protein. Mutations in the PTEN gene have been implicated in several disorders including cancer, diabetes, and Cowden syndrome, among others. It is part of the PI3K-PTEN-AKT-mTOR signaling pathway and is responsible for various metabolic and cellular activities. This pathway is found to be conserved across several eukaryotic systems including yeast, Drosophila melanogaster and Caenorhabditis elegans. While PTEN orthologues have been characterized in ascomycete fungi, it has not been reported in any basidiomycete fungi. The initial part of the current study was aimed at the characterization of PTEN orthologue, ptn1, of Ustilago maydis, a basidiomycete fungus. When the U. maydis, ptn1 gene was either deleted or overexpressed, it was found that the deletion of this gene lead to reduced virulence, spore production, and germination rate; effects of the overexpression were more subtle or not discernable. Previous studies have indicated that two other signaling proteins, Rho1 and Pdc1, are physical interactors of Ptn1. The goal of the latter part of the present study was to explore the functional interaction of Ptn1 with vi Rho1 and Pdc1. Our experiments indicated a functional connection between these genes, especially in processes that are related to stress tolerance, cellular morphology, and virulence of the fungus, suggesting that they may be elements of the same regulatory pathway. We predict that the pdc1 interaction with ptn1 may be the reverse of that of rho1 with ptn1. In addition, our experiments with an orthologue of S6K1, aga1, showed that its deletion leads to some of the same phenotypes seen with that of ptn1, pdc1 and/or rho1. The current phenotypes of these mutant strains when considered in the context of already available information about the role of these genes in other systems indicate that they may be part of a pathway that modulates cytoskeletal rearrangements. vii

show abstract

Section: Figure 23 (A)supporting

confidence: 80%

Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Exploring the role of PTN1 in ustilago maydis.

Pillai¹,

Krishna²

View full text Add to dashboard Cite

show abstract

“…Both enzymes have been found to be resistant to the PDE inhibitor isobutyl-1-methylxanthine (IBMX), and are proposed to be regulated via PKA by phosphorylation. Moreover, diploid cells deleted for one copy of umpde1 display halpoinsuffiency, in that they produce fewer aerial hyphae on charcoal agar and show reduced virulence on maize (Pham et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Ustilago maydis phosphodiesterases play a role in the dimorphic switch and in pathogenicity

et al. 2013

Self Cite

View full text Add to dashboard Cite

Components of the cAMP (cyclic AMP) signalling cascades are conserved from fungi to humans, and are particularly important for fungal dimorphism and pathogenicity. Previous work has described two phosphodiesterases, UmPde1 and UmPde2, in Ustilago maydis which show strong phosphodiesterase activity. We further characterized the biological function(s) of these phosphodiesterases in U. maydis. Specifically, we examined their possible role(s) in regulation of the cAMP-dependent protein kinase A (PKA) pathway and their roles in filamentous growth and pathogenicity. We found that UmPde1, which shares 35 % similarity with Cryptococcus neoformans Pde1, also displays functional homology with this enzyme. UmPde1 complements the capsule-formation defect of C. neoformans strains deleted for Pde1. In U. maydis, the cell morphology of the umpde1 deletion mutant resembled the multiple budding phenotypes seen with the ubc1 mutant, which lacks the regulatory subunit of PKA. Interestingly, on low-ammonium medium, umpde2 deletion strains showed a reduction in filamentation that was comparable to that of ubc1 deletion strains; however, umpde1 deletion strains showed normal filamentation on lowammonium medium. Furthermore, both the ubc1 deletion strain in which the PKA pathway was constitutively active and the umpde1 deletion strains were significantly reduced in pathogenicity, while the umpde2 deletion strains showed a trend for reduced pathogenicity compared with wildtype strains. These data support a role for the phosphodiesterases UmPde1 and UmPde2 in regulating the U. maydis cAMP-dependent PKA pathway through modulation of cAMP levels, thus affecting dimorphic growth and pathogenicity.

show abstract

GTPases in Hyphal Growth

Ranocchi

Amicucci

2021

Encyclopedia of Mycology

View full text Add to dashboard Cite

Haplo-insufficiency for different genes differentially reduces pathogenicity and virulence in a fungal phytopathogen

Cited by 5 publications

References 48 publications

Exploring the role of PTN1 in ustilago maydis.

Exploring the role of PTN1 in ustilago maydis.

Ustilago maydis phosphodiesterases play a role in the dimorphic switch and in pathogenicity

GTPases in Hyphal Growth

Contact Info

Product

Resources

About