Haploid and diploid cell cultures from a haplo-diploid insect

Wahrman, Miryam Z.; Zhu, Sutian

doi:10.1080/07924259.1993.9672336

Cited by 6 publications

(7 citation statements)

References 22 publications

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“…There was no significant difference between the amount of JH produced in the LHB medium and that produced in a modified (pH and osmolarity) Grace's medium. Interestingly, media based on Grace's have been successfully used in several attempts to establish hymenopteran cell lines (Stanley, 1968in Giauffret et al, 1971Wahrman and Zhu, 1993). In Hymenoptera, larval hemolymph contains a relatively low Na+/ K ÷ ratio, very different from the hemolymph of the adult, which is characterized by higher Na ÷ levels (Florkin and Jeuniaux, 1974).…”

Section: Discussionmentioning

confidence: 97%

In vitro biosynthesis of juvenile hormone in larval honey bees: Comparison of six media

Rachinsky

Hartfelder

1998

In Vitro Cell.Dev.Biol.-Animal

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We have formulated a tissue culture medium based on the components of larval honey bee hemolymph. Using an in vitro radiochemical assay to measure juvenile hormone biosynthesis, we compared our larval-based medium to four commercially available media (Grace's, Medium-199; Shields and Sang M3, and Minimum Essential Medium), and a medium based on adult honey bee hemolymph. All media were formulated without methionine. There was no significant difference in the amounts of juvenile hormone produced by the larval medium and Grace's; both of these media, however, were more suitable than the remaining four. Our larval-based tissue culture medium should prove useful in studies aimed at elucidating the underlying hormonal mechanism(s) of caste development in honey bees.

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Section: Discussionmentioning

confidence: 97%

In vitro biosynthesis of juvenile hormone in larval honey bees: Comparison of six media

Rachinsky

Hartfelder

1998

In Vitro Cell.Dev.Biol.-Animal

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“…A primary cell line is a cell line derived from specific insect tissues or organs, cultured on an artificial medium and maintained for a limited time. Primary cell cultures have been established from three hymenopteran species, including an ant (Pseudomyrmex triplarinus), a parasitic wasp (Mormoniella vitripennis), and the honey bee (A. mellifera) ( Table 1) [26][27][28][29]. The longevity of these primary cell cultures was highly variable.…”

Section: Primary Cell Linesmentioning

confidence: 99%

Cell Lines for Honey Bee Virus Research

Guo

Goodman

Stanley

et al. 2020

Viruses

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With ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of the virus load, there is an urgent need to protect honey bees (Apis mellifera) from fatal levels of virus infection and from the non-target effects of insecticides used in agricultural settings. A continuously replicating cell line derived from the honey bee would provide a valuable tool for the study of molecular mechanisms of virus-host interaction, for the screening of antiviral agents for potential use within the hive, and for the assessment of the risk of current and candidate insecticides to the honey bee. However, the establishment of a continuously replicating honey bee cell line has proved challenging. Here, we provide an overview of attempts to establish primary and continuously replicating hymenopteran cell lines, methods (including recent results) of establishing honey bee cell lines, challenges associated with the presence of latent viruses (especially Deformed wing virus) in established cell lines and methods to establish virus-free cell lines. We also describe the potential use of honey bee cell lines in conjunction with infectious clones of honey bee viruses for examination of fundamental virology.

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“…A primary cell line is a cell line derived from specific insect tissues or organs, cultured on artificial medium and maintained for a limited time. Primary cell cultures have been established from three hymenopteran species including an ant (Pseudomyrmex triplarinus), a parasitic wasp (Mormoniella vitripennis) and the honey bee (A. mellifera) ( Table 1) [26][27][28][29]. The longevity of these primary cell cultures was highly variable.…”

Section: Primary Cell Linesmentioning

confidence: 99%

Cell Lines for Honey Bee Virus Research

Guo¹,

Goodman²,

Stanley³

et al. 2020

Preprint

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With ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of virus load, there is an urgent need to protect honey bees (Apis mellifera) from fatal levels of virus infection and from nontarget effects of insecticides used in agricultural settings. A continuously replicating cell line derived from the honey bee would provide a valuable tool for study of molecular mechanisms of virus – host interaction, for screening of antiviral agents for potential use within the hive, and for assessment of the risk of current and candidate insecticides to the honey bee. However, the establishment of a continuously replicating, honey bee cell line has proved challenging. Here we provide an overview of attempts to establish primary and continuously replicating hymenopteran cell lines, methods (including recent results) for establishing honey bee cell lines, challenges associated with the presence of latent viruses (especially Deformed wing virus), in established cell lines and methods to establish virus-free cell lines. We also describe the potential use of honey bee cell lines in conjunction with infectious clones of honey bee viruses for examination of fundamental virology.

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Haploid and diploid cell cultures from a haplo-diploid insect

Cited by 6 publications

References 22 publications

In vitro biosynthesis of juvenile hormone in larval honey bees: Comparison of six media

In vitro biosynthesis of juvenile hormone in larval honey bees: Comparison of six media

Cell Lines for Honey Bee Virus Research

Cell Lines for Honey Bee Virus Research

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