Haploids in the Improvement of Crucifers

Friedt, Wolfgang; Zarhloul, M. Karim

doi:10.1007/3-540-26889-8_10

Cited by 14 publications

(19 citation statements)

References 114 publications

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“…Highly efficient model systems for microspore embryogenesis and subsequent DH plant regeneration have been developed with a number of Brassica species (Custers 2003;da Silva Dias 2003;Ferrie 2003;Hansen 2003). Microspore culture systems are available for all Brassica species within U's triangle and application of this technology has been published (Ferrie and Keller 2004;Friedt and Zarhloul 2005). Tremendous progress has been achieved and microspore culture technology and its applications are being used in breeding programs around the world to develop improved cultivars and to answer fundamental biological questions.…”

Section: Introductionmentioning

confidence: 99%

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Ferrie

Möllers

2010

Plant Cell Tiss Organ Cult

103

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The availability of a highly efficient and reliable microspore culture protocol for many Brassica species makes this system useful for studying basic and applied research questions. Microspores and microspore-derived embryos are ideal targets for modification by mutagenesis and transformation. Regenerated doubled haploid plants are widely used in breeding programs and in genetic studies. Furthermore, the Brassica microspore culture system allows the identification of genomic regions and genes involved in the microspore embryogenic response, spontaneous diploidization and direct embryo to plant conversion. This review summarizes current achievements and discusses future perspectives.

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Section: Introductionmentioning

confidence: 99%

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Ferrie

Möllers

2010

Plant Cell Tiss Organ Cult

103

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“…Although both oilseed rape microspores and MDEs characterise high regeneration potential (Fig. 3) (Cegielska et al, 2002, Friedt andZarhloul 2005;Takahata et al, 2005), only MDEs were able to regenerate post Agrobacterium inoculation (Fig. 4).…”

mentioning

confidence: 98%

“…In this method, two directions of oilseed rape improvement and new cultivar production have been combined -doubled haploids and genetic modification. Doubled haploids, obtained by tissue cultures of microspores and microspore-derived embryos (MDEs), show uniformity and complete homozygosity, thus they are particularly useful for breeding programmes and have been extensively used to produce homozygous breeding lines and cultivars of oilseed rape (Cegielska et al, 2002;Friedt and Zarhloul, 2005;Takahata et al, 2005). DH plants can also be efficiently used in basic studies, for example in genetic analysis and genetic transformation (Cegielska-Taras et al, 2008;Fukuoka et al, 1998;Huang, 1992;Jardinaud et al, 1993;Kazan et al, 1997;Nehlin et al, 2000;Oelck et al, 1991;Swanson & Erickson, 1989;Takahata et al, 2005;Troczyńska et al, 2003).…”

mentioning

confidence: 99%

The Use of Herbicides in Biotech Oilseed Rape Cultivation and in Generation of Transgenic Homozygous Plants of Winter Oilseed Rape (Brassica napus L.)

Cegielska-Taras¹,

Pniewski²

2011

Herbicides - Mechanisms and Mode of Action

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“…It is shown that there is a high correlation between the number of chloroplasts in the stomata guard cells and the ploidy of plants [24]. Thus, the optimization of DHapproach for breeding and seed production remains relevant [25][26][27][28].…”

mentioning

confidence: 99%

NEW GENERATION HYBRIDS OF WHITE CABBAGE (Brassica oleracea L. convar. capitata var. alba DC) BASED ON DOUBLED HAPLOIDS

Pivovarov¹,

Бондарева²,

Шмыкова³

et al. 2017

S-h. biol.

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A b s t r a c tPresently, cabbage breeding is mainly focused on F 1 hybrids necessitating constant parental lines to be obtained. Doubled haploid (DH) technology based on isolated microspore in vitro culture is widely used to produce pure lines of brassica crops. This method allows us to rapidly develop homozygous lines, in contrast to time-consuming traditional breeding for heterosis in cross-pollinating crops which takes 7 to 10 years for annuals and 14 to 20 years for biennial plants. One of the objectives of DH technology is to provide the all possible number of doubled haploid plants that allows more fully encompass the spectrum of genetic recombination, including the recessive locus. The aim of our study was to evaluate economically important traits in white cabbage (Brassica oleracea L. convar. capitata var. alba DC) constant doubled haploid lines of late ripening and to improve the technology for producing DH based F 1 hybrids. Eleven breeding lines of late ripening cabbage were used to obtain doubled haploid lines from microspore in vitro culture. Of the obtained lines, twelve doubled haploid genotypes were selected for further use based on evaluation of ploidy and combining ability. Seed progeny was reproduced by hybridization of regenerated plants in a climatic chamber (2014)(2015). We used the schemes of creating self-incompatible lines and two-line-based hybrids. In the field trials (Moscow region, 2014(Moscow region, -2015, the doubled haploids and their hybrid combinations were compared to the standard (Severyanka F 1 ) for the main valuable characteristics (i.e. the content of dry matter, nitrates, and vitamin C). The field resistance to Fusarium wilt, alternariosis, and pest damage were determined at cabbagehead technical maturity. The resistance to clubroot was assessed under artificial infection. There was a direct relationship of the average number of chromosomes to the number of chloroplasts in the stomata guard cells and the length of guard cells. The frequency of spontaneous doubling of the chromosomes numbers varied from 50.0 % to 87.5 % in different geotypes. A total of 11 to 73 % produced lines were high self-incompatible. Their geitonogamic pollination in the topcrosses resulted in 42 hybrid combinations. The model of F 1 hybrid most fully responding to consumer market demands was developed. Ten promising hybrid combinations which matched the model parameters in two-year field testing were recommended for variety testing. Hybrids were characterized by uniformity, high biochemical quality, the resistance to major diseases and pests and the yield of 104.60±8.27 t/ha. The dry matter content reached to 10.5 %, the sugar content was about 4.21-5.10 %, and ascorbic acid level ranged from 21.12 to 38.70 mg%. Both the highest level of ascorbic acid (92.0 mg%) and the smallest nitrate accumulation (33 mg/kg) were characteristic of one hybrid combination.Keywords: white cabbage, Brassica oleracea L., doubled haploid lines, heterosis F 1 hybrids, in vitro isolated microspore culture, self-incomp...

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Haploids in the Improvement of Crucifers

Cited by 14 publications

References 114 publications

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

The Use of Herbicides in Biotech Oilseed Rape Cultivation and in Generation of Transgenic Homozygous Plants of Winter Oilseed Rape (Brassica napus L.)

NEW GENERATION HYBRIDS OF WHITE CABBAGE (Brassica oleracea L. convar. capitata var. alba DC) BASED ON DOUBLED HAPLOIDS

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