Haplotype analysis of Ethiopian bread wheat (Triticum aestivum) cultivars and elite lines for yellow rust resistance genes using linked molecular markers

Dawit, Woubit; Badebo, Ayele; Hunde, Bekele; Kassa, Daniel; RÃ¶der, M. S.

doi:10.5897/ajb2018.16539

Cited by 2 publications

(1 citation statement)

References 33 publications

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“…tritici populations is mainly due to the long-distance migration, high mutation, somatic recombination, and selection of the cultivars (Wan et al, 2004;Chen, 2005 Yr3a, Yr3b, Yr4a, Yr6, Yr6+2, Yr7, Yr8, Yr9, Yr9+2, Yr10, Yr17, Yr24, Yr27, Yr32, YrA, YrCV, YrSD and YrSU, has been reported worldwide, including Egypt (Dawit et al, 2012;Shahin, 2017;Draz, 2019). Dawit et al (2019) found that the differential lines carrying Yr9, Yr8, Yr6 and Yr7 had the highest rust severity of more than 80 S. Nazari and EL Amil (2013) reported that NILs Yr6, Yr7, and Yr9 responded with high infection types. The Yr9 was found among genes that are susceptible to 70-100% of sixty-one isolates of P. striiformis f. sp.…”

Section: Discussionmentioning

confidence: 99%

Race specificity of stripe rust resistance in relation to susceptibility of Egyptian wheat cultivars

Shahin

Draz

Esmail

2020

Egyptian Journal of Phytopathology

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ace -specific resistance of stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) in relation to susceptibility of Egyptian wheat cultivars were investigated during two growing seasons 2017/18 and 2018/19 in Egypt. High levels of adult plant susceptibility were recorded in the second season in comparison with that of the first season. Out of 20 cultivars tested, susceptibility (S) up to 80 S was recorded with only 8 cultivars and 4 resistance genes (Yr) in the first season. However, it was recorded with 14 cultivars (reached 90 S) and 7 Yr genes (up to 80 S) in the second one. The highest susceptibility was recorded with Sids-12 and Gemmeiza-11 (90 S), followed by Misr-1 (80 S), Misr-2 (70 S), Giza-163 (60 S), Yr7 and Yr9 (80 S). Cultivars, Misr-1, Misr-2, Gemmeiza-5, Gemmeiza-12, Giza-167, and Shandweel-1, exhibiting moderate resistance (MR) in the first season, lost their resistance in the second one. Nine Yr genes Yr2,

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Section: Discussionmentioning

confidence: 99%

Race specificity of stripe rust resistance in relation to susceptibility of Egyptian wheat cultivars

Shahin

Draz

Esmail

2020

Egyptian Journal of Phytopathology

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Identification of Stripe Rust Resistance in Ethiopian Durum Wheat by Phenotypic Screening and Kompetitive Allele Specific PCR (KASP) SNP Markers

Alemu¹,

Badebo²,

Tesfaye³

et al. 2019

J Plant Pathol Microbiol

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Stripe (Yellow) rust caused by Puccinia striiformis f.sp. tritici (Pst) is one of the most devastating diseases of wheat in the highlands of Ethiopia. Improved cultivars often lose their resistance due to occurrence of new virulent races which overcome the genes and make the cultivars out of production. Therefore, identification of new sources of resistance genes helps in battling yellow rust and maximizes wheat production in Ethiopia. In this study, 300 durum wheat lines (landraces & cultivars) were screened with three virulent isolates (Pst_Is1, Pst_Is4 and Pst_Is8) for seedling resistance using Infection Type (IT) scoring method. The lines were also screened with 16 KASP-based SNP markers linked to 7 Yr genes already identified in various studies. Highly resistant infection type (IT: 0-3) to Pst_Is1, Pst_Is4, and Pst_Is8 was exhibited by 59.3%; 67.3%; and 46.3% of the lines, respectively. 124 lines constantly exhibited high level of resistance to all three isolates. The majority (96.8%) of the resistant lines are landraces while four (3.2%) are commercial cultivars (Cocorit/71, Yerer, Obsa and Dire). In the molecular screening 12 of the markers gave clear amplifications in the controls and the tested lines. Yr7, Yr15 and YrSp were detected in 81.7%, 88.3% and 0.7% of the lines respectively while Yr1, Yr17 and Yr36 were not detected. Detection frequency was higher in landraces (58.7%) than in cultivars (32.8%). Gene combinations frequency was the highest (72.7%) for Yr7+Yr15 followed by Yr15+YrSp (0.3%). Overall, this study has resulted in detection of genes Yr15 and YrSp, which are potential candidates for marker assisted breeding for Pst resistance in wheat. Besides, it has shown that resistant source identification and detection of genes can be facilitated through combined application of phenotyping and molecular screening.

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Haplotype analysis of Ethiopian bread wheat (Triticum aestivum) cultivars and elite lines for yellow rust resistance genes using linked molecular markers

Cited by 2 publications

References 33 publications

Race specificity of stripe rust resistance in relation to susceptibility of Egyptian wheat cultivars

Race specificity of stripe rust resistance in relation to susceptibility of Egyptian wheat cultivars

Identification of Stripe Rust Resistance in Ethiopian Durum Wheat by Phenotypic Screening and Kompetitive Allele Specific PCR (KASP) SNP Markers

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