Haplotype analysis of the genes encoding glutamine synthetase plastic isoforms and their association with nitrogen‐use‐ and yield‐related traits in bread wheat

Li, Xin-Peng; Zhao, Xueqiang; He, Xue; Zhao, Guangyao; Li, Bin; Liu, Dongcheng; Zhang, Aimin; Zhang, Xueyong; Tong, Yiping; Li, Zhensheng

doi:10.1111/j.1469-8137.2010.03490.x

Cited by 66 publications

(70 citation statements)

References 45 publications

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“…In contrast, the SNPs we have identified map to 2DL and are not in LD with previously reported Ppd-D1 marker data for these genotypes (Grogan et al 2016). Glutamine synthase 2 genes are located in distal deletion bins on the long arms of group 2 chromosomes and are potential candidates for future investigation, given their important role in previous studies (Li et al 2011).…”

Section: Association Analysiscontrasting

confidence: 46%

Variation for nitrogen use efficiency traits in current and historical great plains hard winter wheat

et al. 2017

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Wheat genotypes that efficiently capture and convert available soil nitrogen into harvested grain protein are key to sustainably meeting the rising global demand for grain protein. The purposes of this study were: to characterize the genetic variation for nitrogen use efficiency (NUE) traits within hard winter wheat adapted to the Great Plains of the United States and evaluate trends in the germplasm with year of release; to explore relationships among traits that may be used for selection within breeding programs; and to identify quantitative trait loci associated with NUE traits in this germplasm. NUE traits were measured in a panel of 299 hard winter wheat genotypes, representing historically important and contemporary germplasm, from across the growing region. Trials were grown in two years at two levels of nitrogen fertility. Genotype and genotype 9 year interaction effects were highly significant for NUE traits, while genotype 9 nitrogen rate interactions were non-significant. Strong genetic correlations of plant height and flowering date with NUE traits were observed. Wheat breeders have improved NUE: the subset of 183 genotypes that were released as cultivars after 1960 demonstrated significant trends with year of release for improved grain N yield, grain yield, nitrogen harvest index, nitrogen uptake efficiency, nitrogen utilization efficiency, and post-anthesis nitrogen uptake. In genome-wide association analyses, plant height and flowering date were important covariates in the mixed models, and plant height and flowering date substantially explained the variation in NUE traits in this germplasm. Marker-trait associations were identified that may prove useful in breeding.

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Section: Association Analysiscontrasting

confidence: 46%

Variation for nitrogen use efficiency traits in current and historical great plains hard winter wheat

et al. 2017

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“…the expression of TaGS2 encoding plastid-located GS (Li et al, 2011). Overexpression of TaNAC2-5A caused the up-regulation of TaGS2 (representing the summed expression of TaGS2-2A, TaGS2-2B, and TaGS2-2D) in both shoots and roots (Fig.…”

Section: Overexpression Of Tanac2-5a Up-regulates the Expression Of Nmentioning

confidence: 94%

“…These studies clearly demonstrate that efficient root-toshoot transport and remobilization of N are required to support plant growth and achieve high yields. In major cereal crops, the N assimilation genes NITRATE REDUCTASE, GLUTAMINE SYNTHETASE (GS), and GLUTAMATE SYNTHASE are known to colocalize with QTLs affecting grain yield and NUE (Hirel et al, 2001;Obara et al, 2001Obara et al, , 2004Habash et al, 2007;Coque et al, 2008;Zhang et al, 2010;Li et al, 2011;Quraishi et al, 2011). Furthermore, a number of studies have shown that ectopic expression of the genes involved in primary N metabolism and amino acid biosynthesis can increase N uptake and assimilation and/or promote plant growth and yield (McAllister et al, 2012).…”

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confidence: 99%

The nitrate inducible NAC transcription factor TaNAC2-5A controls nitrate response and increases wheat yield

et al. 2015

Plant Physiol.

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Nitrate is a major nitrogen resource for cereal crops; thus, understanding nitrate signaling in cereal crops is valuable for engineering crops with improved nitrogen use efficiency. Although several regulators have been identified in nitrate sensing and signaling in Arabidopsis (Arabidopsis thaliana), the equivalent information in cereals is missing. Here, we isolated a nitrateinducible and cereal-specific NAM, ATAF, and CUC (NAC) transcription factor, TaNAC2-5A, from wheat (Triticum aestivum). A chromatin immunoprecipitation assay showed that TaNAC2-5A could directly bind to the promoter regions of the genes encoding nitrate transporter and glutamine synthetase. Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate and, hence, increased the root's ability to acquire nitrogen. Furthermore, we found that TaNAC2-5A-overexpressing transgenic wheat lines had higher grain yield and higher nitrogen accumulation in aerial parts and allocated more nitrogen in grains in a field experiment. These results suggest that TaNAC2-5A is involved in nitrate signaling and show that it is an exciting gene resource for breeding crops with more efficient use of fertilizer.

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“…In addition, TaSus2 could influence the responses of KL and KW to N stress, but it had no effect on the response of per-plant yield (Cui et al 2014a). Glutamine synthetase (GS2) is a key enzyme involved in plant N metabolism that is responsible for the first step of ammonium assimilation and transformation into glutamine; thus, it plays a key roles in the growth, N use and yield potential of cereal crops, including wheat (Gadaleta et al 2011;Li et al 2011). Polyphenol oxidase (PPO) activity is highly correlated with the undesirable browning of wheat-based end products, especially Asian noodles (He et al 2007;Liu et al 2012).…”

Section: Novel Stable Qtls and Qtl Co-segregation With Known Genesmentioning

confidence: 99%

“…GPC, particularly glutenin and gliadin content is controlled by a complex genetic system and is strongly affected by the environment. It is an important factor for pasta-making and baking quality and is strongly correlated with WGC, DT, ABS, and ZEL (Zanetti et al 2001;Nelson et al 2006;Suprayogi et al 2009;Li et al 2011). KH, one of the most important determinants of milling yield, has profound effects on the milling, baking and enduse quality of bread wheat ).…”

Section: Introductionmentioning

confidence: 99%

QTL detection for wheat kernel size and quality and the responses of these traits to low nitrogen stress

et al. 2015

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QTLs for tolerance to N stress, 27 and 14 of which were stable across the tested environments, respectively. These QTLs were distributed across all wheat chromosomes except for chromosomes 3A, 4D, 6D, and 7B. Eleven QTL clusters that simultaneously affected kernel size-and quality-related traits were identified. At nine locations, 25 of the 49 QTLs for N deficiency tolerance coincided with the QTLs for kernel characteristics, indicating their genetic independence. The feasibility of indirect selection of a superior genotype for kernel size and quality under high-N conditions in breeding programs designed for a lower input management system are discussed. In addition, we specified the functions of Glu-A1, Glu-B1, Glu-A3, Glu-B3, TaCwi-A1, TaSus2, TaGS2-D1, PPO-D1, Rht-B1, and Ha with regard to kernel characteristics and the sensitivities of these characteristics to N stress. This study provides useful information for the genetic improvement of wheat kernel size, quality, and resistance to N stress. Abbreviations GPCGrain protein content WGC Wet gluten content AbstractKey message QTLs for kernel characteristics and tolerance to N stress were identified, and the functions of ten known genes with regard to these traits were specified. Abstract Kernel size and quality characteristics in wheat (Triticum aestivum L.) ultimately determine the end use of the grain and affect its commodity price, both of which are influenced by the application of nitrogen (N) fertilizer. This study characterized quantitative trait loci (QTLs) for kernel size and quality and examined the responses of these traits to low-N stress using a recombinant inbred line population derived from Kenong 9204 × Jing 411. Phenotypic analyses were conducted in five trials that each included lowand high-N treatments. We identified 109 putative additive QTLs for 11 kernel size and quality characteristics and 49Communicated by I. Mackay.F. Cui, X. Fan, and M. Chen contributed equally to this work. Electronic supplementary materialThe online version of this article

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Haplotype analysis of the genes encoding glutamine synthetase plastic isoforms and their association with nitrogen‐use‐ and yield‐related traits in bread wheat

Cited by 66 publications

References 45 publications

Variation for nitrogen use efficiency traits in current and historical great plains hard winter wheat

Variation for nitrogen use efficiency traits in current and historical great plains hard winter wheat

The nitrate inducible NAC transcription factor TaNAC2-5A controls nitrate response and increases wheat yield

QTL detection for wheat kernel size and quality and the responses of these traits to low nitrogen stress

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