Hard tissue regeneration capacity of apical pulp derived cells (APDCs) from human tooth with immature apex

Differentiation Potential of Dental Papilla, Dental Pulp, and Apical Papilla Progenitor Cells

“…In the pilot study of Sonoyama et al (45), the apical papilla appears to be histologically distinct from the pulp, from which another mesenchymal stem cell population, the apical papilla stem cells, could be isolated. Various groups have characterized these cells by using histologic, immunohistologic, and immunofluorescent analyses and concluded that they are multipotent mesenchymal stem cells expressing various markers (48)(49)(50)(51) and showed their abilities to differentiate into chondroblasts, osteoblasts, adipocytes, etc.…”

Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells cultured with umbilical cord blood-derived autoserum

“…After washing, the decalcified samples were embedded in paraffin, cut to about 3-mm sections, stained with HE, and observed with an optical microscope. Immunohistochemical staining followed published procedures (Abe et al, 2008). Namely, specimens were stained with the anti-human osteocalcin (OC) antibody and the anti-human mitochondrial antibody before assessment with a fluorescence microscope.…”

Dental Stem Cells: Sources and Potential Applications

“…These structures progressively express differentiation markers, including DSPP, BSP, OCN, ALP, thus showing the characteristics of osteodentin [52]. When SCAP are combined with HA scaffolds and implanted subcutaneously in immunocompromised rats, bone-and dentin-like mineralized tissues are formed within 12 weeks following transplantation [53]. Furthermore, when SCAP were seeded onto synthetic scaffolds, inserted into tooth fragments, and then transplanted into immunodeficient mice, a pulp-like tissue with wellestablished vascularity was induced and a continuous layer of dentin-like tissue was deposited onto the canal dentinal wall [24].…”