2011
DOI: 10.1364/ol.36.001269
|View full text |Cite
|
Sign up to set email alerts
|

Hard x-ray Zernike microscopy reaches 30 nm resolution

Abstract: Since its invention in 1930, Zernike phase contrast has been a pillar in optical microscopy and more recently in x-ray microscopy, in particular for low-absorption-contrast biological specimens. We experimentally demonstrate that hard-x-ray Zernike microscopy now reaches a lateral resolution below 30 nm while strongly enhancing the contrast, thus opening many new research opportunities in biomedicine and materials science.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

0
46
1

Year Published

2011
2011
2015
2015

Publication Types

Select...
6
2

Relationship

1
7

Authors

Journals

citations
Cited by 54 publications
(47 citation statements)
references
References 11 publications
0
46
1
Order By: Relevance
“…Another common artifact in phase-contrast microscopy using the phase ring is shade-off that reduces the contrast of the sample center, and which is typically shown in large flat samples. 3 Remarkably, this was not shown in our case. Its absence can be considered to be owed mainly to the phase plate, though artifact issues in large-sample x-ray microscopy will need further study.…”
Section: Imaging Experimentscontrasting
confidence: 73%
See 1 more Smart Citation
“…Another common artifact in phase-contrast microscopy using the phase ring is shade-off that reduces the contrast of the sample center, and which is typically shown in large flat samples. 3 Remarkably, this was not shown in our case. Its absence can be considered to be owed mainly to the phase plate, though artifact issues in large-sample x-ray microscopy will need further study.…”
Section: Imaging Experimentscontrasting
confidence: 73%
“…[1][2][3][4] Notably, unlike electron microscopy, and thanks to the high-transmission characteristic of hard x-rays, this microscopy modality allows for in vivo and in situ experiments with large bio-samples. However, its weak absorption negatively affects image contrast.…”
Section: Introductionmentioning
confidence: 99%
“…1c, with a high spatial resolution (~50 nm per pixel) without using any contrast agents. The interference bright and dark fringes at each interface, originating from the Zernike phase contrast3738, allowed us to clearly identify each interface3940. We for the first time found that wetting ridges in large drops ( r ≈1 mm) have bent cusps (Fig.…”
Section: Resultsmentioning
confidence: 78%
“…In projection mode at 17-29 keV, with inherent propagation-based phase contrast, a spatial resolution of 180 nm in 3D has been reported in Requena et al (2009). In full-field mode and Zernike phase contrast a spatial resolution of 30 nm was demonstrated for the 2D case in Chen et al (2011) and beyond 200 nm for 3D in and Takeuchi et al (2011). As a consequence and differently from soft X-ray microscopes these instruments are well suited for both biological and material science applications.…”
Section: Introductionmentioning
confidence: 96%