This paper is to analyze the microstructure, microhardness, tensile and fatigue properties of the welded joints performed by a fiber laser on the 22MnB5 and Q235 steels in similar and dissimilar combinations. The result shows that the weld zone (WZ) consisted of lathy martensite, and the heat affected zone (HAZ) of 22MnB5 could be divided into 3 parts: quenched zone, incomplete quenched zone and the tempered zone which didn't appear in the Q235 HAZ. The WZ had the highest hardness, while the HAZ was very narrow with a very fast drop in hardness. A soft zone existed in the HAZ of 22MnB5, and it was absent on the Q235 side. With the welding speed increasing, the martensite in the WZ became thinner and shorter, and the width of HAZ decreased. All the tensile and fatigue specimens of the similar 22MnB5 welded joints were broken in the HAZ. The base metal (BM) had a higher fatigue life than the similar 22MnB5 welded joints. The fatigue fracture contained 3 parts: crack initiation region which occurred from the specimen surface, crack propagation region and the final fast fracture region.