Hatching status before embryo transfer is not correlated with implantation rate in chromosomally screened blastocysts

Rodriguez-Purata, J.; Gingold, Julian A.; Lee, Joseph; Whitehouse, M.C.; Slifkin, Robert F.; Briton-Jones, Christine; Copperman, A.B.; Sandler, B.

doi:10.1093/humrep/dew205

Cited by 16 publications

(14 citation statements)

References 38 publications

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“…Patients underwent standard controlled ovarian hyperstimulation for in vitro fertilizaiton with a down-regulation protocol with leuprolide acetate, an antagonist protocol, or a Microflare protocol [18]. Oocyte maturation was induced with recombinant human chorionic gonadotropin alone (Ovidrel 250 mg; EMD Serono) or with a ‘dual trigger’ using leuprolide acetate 2 mg (Lupron; AbbVie Laboratories) and human chorionic gonadotropin 1,000 IU (Novarel; Ferring Pharmaceuticals).…”

Section: Methodsmentioning

confidence: 99%

“…After 60 seconds, embryos were moved into 0.5 mol/L sucrose in tissue culture medium 199 + 20% synthetic serum substitute at room temperature for 3 minutes. Last, embryos were washed for 5 min and then for 1 min with tissue culture medium 199 + 20% synthetic serum substitute at room temperature [18]. After the embryos were rewarmed, the appearances of the blastomeres and zona pellucida, and the ability of the blastocoel to re-expand, were assessed to confirm embryo survival.…”

Section: Methodsmentioning

confidence: 99%

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Paternal contribution to embryonic competence

Stein

Ukogu

Ganza

et al. 2019

Cent european J Urol

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IntroductionThe effect of paternal characteristics on embryo development and the outcome of preimplantation genetic testing for aneuploidy have not been extensively explored. This study investigates the association of sperm parameters, insemination, and extraction techniques, with the rate of embryo aneuploidy. This study sought to evaluate the association between male factor infertility and embryo aneuploidy.Material and methodsPatients underwent in vitro fertilization using intracytoplasmic sperm injection, with preimplantation genetic testing for aneuploidy. Patients were divided into four groups by sperm parameters: Group A: oligozoospermia (sperm concentration <10 million, morphology > 4%); Group B: teratozoospermia (sperm concentration >10 million, morphology <4%); Group C: oligozoospermia and teratozoospermia (sperm concentration <10 million, morphology <4%); Group D: controls. Additionally, couples were divided into three categories by days of abstinence: Group A: <2; Group B: 2-7; and Group C: >7.ResultsA total of 4108 in vitro fertilization cycles with preimplantation genetic testing for aneuploidy were analyzed. After controlling for parental age and follicle count, the rate of embryo aneuploidy was not affected by duration of abstinence, sperm parameters, or the source of the sperm sample.ConclusionsNumerous factors related to sperm source and quality were evaluated, and a minimal influence on the rate of embryo aneuploidy was observed.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Paternal contribution to embryonic competence

Stein

Ukogu

Ganza

et al. 2019

Cent european J Urol

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“…Although there is an increasing number of studies supporting improved clinical outcomes after FETs (Roque et al 2013, Evans et al 2014, fresh ET protocols are typically more affordable, require little to no additional medications and allow the patients immediate transfer. However, in the context of PGT cycles, a successful fresh day 6 transfer approach obliges not only that expanded blastocysts be available on the morning of day 5 (Rodriguez-Purata et al 2016b), but also that at least one of these biopsied embryos is euploid, thereby also reducing the probability for a transfer in that cycle.…”

Section: Fresh Vs Fetmentioning

confidence: 99%

Ovarian stimulation for preimplantation genetic testing

Rodriguez-Purata

Martínez

2019

Reproduction

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A narrative review of the management of controlled ovarian stimulation in patients undergoing preimplantation genetic testing is presented. An electronic search was performed to identify research publications that addressed ovarian stimulation and preimplantation genetic testing published until December 2017. Studies were classified in decreasing categories: randomized controlled trials, prospective controlled trials, prospective non-controlled trials, retrospective studies and experimental studies. The aim of controlled ovarian stimulation has shifted from obtaining embryos available for transfer to yielding the maximum embryos available for biopsy to increase the odds of achieving one euploid embryo available for transfer, without the distress of inducing ovarian hyperstimulation syndrome or inadequate endometrium receptivity as vitrification and deferred embryo transfer usually will be planned. The present narrative review summarizes all treatment-related variables as well as stimulation strategies after controlled ovarian stimulation that could help patients undergoing an in vitro fertilization cycle coupled with preimplantation genetic testing, including the number of oocytes needed to achieve one healthy live birth, oral contraceptive pill usage, the role of mild ovarian stimulation or random-start stimulation, the stimulation protocol and type of gonadotropin of choice, the novel progesterone protocols, agonist or dual trigger as a final oocyte maturation trigger, the accumulation of oocytes/embryos and the optimal interval before proceeding with a subsequent controlled ovarian stimulation or the optimal medication to link stimulation cycles. The discussion is being presented according to how questions are posed in clinical practice. The aim of ovarian stimulation has shifted from obtaining embryos available for transfer to yielding the maximum embryos available for biopsy to increase the odds of achieving one euploid embryo available for transfer.

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“…Patients underwent conventional COH for IVF as described previously elsewhere (19,20). Oocyte final maturation was induced with recombinant human chorionic gonadotropin (hCG) alone (Ovidrel; EMD Serono) or with 2 mg of leuprolide acetate (Lupron; AbbVie Laboratories) concomitant with 1,000 IU hCG (Novarel; Ferring Pharmaceuticals) in patients at risk of ovarian hyperstimulation syndrome.…”

Section: Stimulation Protocolmentioning

confidence: 99%

“…Embryo culture and biopsy techniques. Embryos were cultured up to the blastocyst stage as previously described elsewhere (19,20). On day 3 of embryo development, all embryos underwent laser-assisted hatching via creating a 25-30 Mm opening in the zona pellucida with a 200-300 ms pulse ZILOS-tk Laser (Hamilton Thorne Biosciences) to facilitate posterior trophectoderm herniation.…”

Section: Laboratory Proceduresmentioning

confidence: 99%