Hbv研究常用的动物模型

Guo, Weina; Zhu, Bin; Ai, Ling; Yang, Dongliang; Wang, Baoju

doi:10.24272/j.issn.2095-8137.2018.013

Cited by 31 publications

(7 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experimental MmPV1 infections cause cutaneous papillomas that, similar to β-HPV induced lesions, can progress to cutaneous squamous cell carcinomas upon UV exposure [23]. In the case of HBV, duck and wood-chuck hepadnaviruses are used in their natural hosts as models of HBV replication and pathogenesis, but, like the mouse polyoma, herpes and papillomaviruses, they do not replicate all features of human HBV infection [24]. …”

Section: Oncogenic Viruses Are Species-specificmentioning

confidence: 99%

More than just oncogenes: mechanisms of tumorigenesis by human viruses

Gaglia

Münger

2018

Current Opinion in Virology

View full text Add to dashboard Cite

Most humans are infected with at least one of the known human cancer viruses during their lifetimes. While the initial infection with these viruses does not cause major disease, infected cells can acquire cancer hallmarks, particularly upon immunosuppression or exposure to co-carcinogenic stimuli. Even though cancer formation represents a rare outcome of a viral infection, approximately one out of eight human cancers has a viral etiology. Viral cancers present unique opportunities for prophylaxis, diagnosis, and therapy, as demonstrated by the success of HBV and HPV vaccines and HCV antivirals in decreasing the incidence of tumors that are caused by these viruses. Here we review common characteristics and mechanisms of action of the human oncogenic viruses.

show abstract

Section: Oncogenic Viruses Are Species-specificmentioning

confidence: 99%

More than just oncogenes: mechanisms of tumorigenesis by human viruses

Gaglia

Münger

2018

Current Opinion in Virology

View full text Add to dashboard Cite

show abstract

“…It is important to note, however, that a specific limitation was associated with this study in that rats cannot be naturally transfected with human hepatitis virus [ 44 ], and although we successfully adopted the BCG immune liver injury rat model, which is recognized by domestic and foreign scholars to simulate the immune liver injury caused by viral hepatitis, species differences and models limitations do exist. Hence, transgenic rat studies will be used for follow-up experiments to further explore the molecular mechanism.…”

Section: Discussionmentioning

confidence: 99%

Involvement of NF-κB in the reversal of CYP3A down-regulation induced by sea buckthorn in BCG-induced rats

Liu

Wang

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

Previous studies reported that sea buckthorn (Hippophae rhamnoides L., Elaeagnaceae, HRP) exhibits hepatoprotective effects via its anti-inflammatory and antioxidant properties as well as its inhibitory effects on collagen synthesis. However, it is unclear whether this hepatoprotective effect is also achieved by regulating liver drug metabolism enzyme pathways. Herein, we examined the regulatory effect of HRP on cytochrome P450 3A (CYP3A) in rats with immune liver injury, and explored the molecular mechanism of its hepatoprotective effect. Rat models of immunological liver injury were induced by intravenous injections of Bacillus Calmette-Guerin (BCG; 125 mg kg-1 ; 2 wks). Specific protein levels were detected by ELISA or western blot, and CYP3A mRNA expression was detected by RT-PCR. High-performance liquid chromatography (HPLC) detected relative changes in CYP3A metabolic activity based on the rates of 1-hydroxylation of the probe drug midazolam (MDZ). BCG pretreatment (125 mg kg-1) significantly down-regulated liver CYP3A protein expression compared with the control, metabolic activity, and transcription levels while up-regulating liver NF-κB, IL-1β, TNF-α and iNOS. HRP intervention (ED 50 : 78 mg kg-1) moderately reversed NF-κB, inflammatory cytokines, and iNOS activation in a dose-dependent manner (P < 0.05), and suppressed CYP3A down-regulation (P < 0.05); thereby partially alleviating liver injury. During immune liver injury, HRP may reverse CYP3A downregulation by inhibiting NF-κB signal transduction, and protect liver function, which involves regulation of enzymes transcriptionally, translationally and post-translationally. The discovery that NF-κB is a molecular target of HRP may initiate the development and optimization of a clinical therapeutic approach to mitigate hepatitis B and other immunity-related liver diseases.

show abstract

“…They can be divided into two main groups: Animals expressing single antigens or the full viral genome and humanized animals (Section “Humanized animals”) ( Figure 2 C). Animals from the first group usually include those expressing HBsAg [ 103 ], HbcAg [ 104 ], HbeAg [ 105 ], HBx [ 106 ], HCV envelope genes [ 107 ], HCV core [ 108 ] or the full HBV or HCV viral genome [ 109 , 110 , 111 , 112 ].…”

Section: Various In Vivo Approaches In Hepatotropic Virus Modelingmentioning

confidence: 99%

Modeling Hepatotropic Viral Infections: Cells vs. Animals

Rad

Zahmatkesh

Bikmulina

et al. 2021

Cells

View full text Add to dashboard Cite

The lack of an appropriate platform for a better understanding of the molecular basis of hepatitis viruses and the absence of reliable models to identify novel therapeutic agents for a targeted treatment are the two major obstacles for launching efficient clinical protocols in different types of viral hepatitis. Viruses are obligate intracellular parasites, and the development of model systems for efficient viral replication is necessary for basic and applied studies. Viral hepatitis is a major health issue and a leading cause of morbidity and mortality. Despite the extensive efforts that have been made on fundamental and translational research, traditional models are not effective in representing this viral infection in a laboratory. In this review, we discuss in vitro cell-based models and in vivo animal models, with their strengths and weaknesses. In addition, the most important findings that have been retrieved from each model are described.

show abstract

Hbv研究常用的动物模型

Cited by 31 publications

References 67 publications

More than just oncogenes: mechanisms of tumorigenesis by human viruses

More than just oncogenes: mechanisms of tumorigenesis by human viruses

Involvement of NF-κB in the reversal of CYP3A down-regulation induced by sea buckthorn in BCG-induced rats

Modeling Hepatotropic Viral Infections: Cells vs. Animals

Contact Info

Product

Resources

About