HC-Pro protein of sugar cane mosaic virus interacts specifically with maize ferredoxin-5 in vitro and in planta

Cheng, Yuqin; Liu, Zhongmei; Xu, Jian; Zhou, Tao; Wang, Meng; Chen, Yuting; Li, Huaifang; Fan, Zaifeng

doi:10.1099/vir.0.2008/001271-0

Cited by 91 publications

(70 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, infections with SCMV or some other viruses are known to alter the transcript levels of nuclear genes encoding chloroplastlocalized proteins (Yang et al, 2007;Cheng et al, 2008;Mochizuki et al, 2014;Chen et al, 2017). Meanwhile, the modified transcript levels of particular host chloroplast proteins are reported to alter the accumulation of viruses.…”

Section: Zmvde Plays a Positive Role Against Scmv Infectionmentioning

confidence: 99%

“…The maize cDNA library screen and the identification of positive interactions were performed as described previously (Cheng et al, 2008). Yeast protein extracts were prepared using the SDS protein extraction method according to the manufacturer's protocols (Clontech).…”

Section: Y2h Assaymentioning

confidence: 99%

“…To identify maize proteins that interacted with SCMV HC-Pro, a Y2H screen of a maize leaf cDNA library (Cheng et al, 2008) was performed using SCMV HC-Pro as a bait. As a result, five identical, independent clones in the screen were obtained, and a BLAST query of the deduced amino acid sequences revealed that these sequences are closely related to maize VDE and that all clones had a conserved VDE superfamily domain (Fig.…”

Section: The Chloroplast Protein Zmvde Interacted With Scmv Hc-pro VImentioning

confidence: 99%

“…HC-Pro also participates in symptom development, genome replication, and virus movement (Maia et al, 1996;Syller, 2006), whereas these capacities should be derived from its ability to suppress RNA silencing (Kasschau and Carrington, 2001;Díaz-Pendón and Ding, 2008). Furthermore, HC-Pros from different viruses have been reported to interact with various host factors and affect their functions, such as the ubiquitin/26S proteasome system (Ballut et al, 2005), the chloroplast precursor of ferredoxin-5 (Cheng et al, 2008), and chloroplast protein 1-deoxy-D-xylulose-5-phosphate synthase (Li et al, 2015).…”

mentioning

confidence: 99%

See 3 more Smart Citations

A Violaxanthin Deepoxidase Interacts with a Viral Suppressor of RNA Silencing to Inhibit Virus Amplification

et al. 2017

Self Cite

View full text Add to dashboard Cite

RNA silencing plays a critical role against viral infection. To counteract this antiviral silencing, viruses have evolved various RNA silencing suppressors. Meanwhile, plants have evolved counter-counter defense strategies against RNA silencing suppression (RSS). In this study, the violaxanthin deepoxidase protein of maize (Zea mays), ZmVDE, was shown to interact specifically with the helper component-proteinase (HC-Pro; a viral RNA silencing suppressor) of Sugarcane mosaic virus (SCMV) via its mature protein region by yeast two-hybrid assay, which was confirmed by coimmunoprecipitation in Nicotiana benthamiana cells. It was demonstrated that amino acids 101 to 460 in HC-Pro and the amino acid glutamine-292 in ZmVDE mature protein were essential for this interaction. The mRNA levels of ZmVDE were down-regulated 75% to 65% at an early stage of SCMV infection. Interestingly, ZmVDE, which normally localized in the chloroplasts and cytoplasm, could relocalize to HC-Pro-containing aggregate bodies in the presence of HC-Pro alone or SCMV infection. In addition, ZmVDE could attenuate the RSS activity of HC-Pro in a specific protein interaction-dependent manner. Subsequently, transient silencing of the ZmVDE gene facilitated SCMV RNA and coat protein accumulation. Taken together, our results suggest that ZmVDE interacts with SCMV HC-Pro and attenuates its RSS activity, contributing to decreased SCMV accumulation. This study demonstrates that a host factor can be involved in secondary defense responses against viral infection in monocot plants.

show abstract

Section: Zmvde Plays a Positive Role Against Scmv Infectionmentioning

confidence: 99%

Section: Y2h Assaymentioning

confidence: 99%

Section: The Chloroplast Protein Zmvde Interacted With Scmv Hc-pro VImentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A Violaxanthin Deepoxidase Interacts with a Viral Suppressor of RNA Silencing to Inhibit Virus Amplification

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Como se interactuando con proteínas codifi cadas por SCMV. Por ejemplo las interacciones entre la elongina C y la proteína viral VPg [23], o la interacción entre la Ferredoxina-5 con la proteína viral HC-Pro [24]. Sin embargo la interacción de proteínas del hospedero con las regiones no traducidas ha sido menos estudiada, a pesar de la importante función de estas regiones en el proceso de traducción en virus, que no presentan una estructura caperuza (cap) 7-metilguanosina (m7GpppG) en el extremo 5´ como es el caso de los ARN mensajeros de eucariotas.…”

Section: Resultados Y Análisisunclassified

Exploración de la interacción entre la región 5´UTR del Sugarcane Mosaic Virus (SCMV) y proteínas del hospedero maíz

Chaves-Bedoya

2017

Respuestas

View full text Add to dashboard Cite

Resumen Antecedentes: El virus del mosaico de la caña de azúcar, SCMV (Potyviridae) es un miembro de la gran familia de virus de ARN de cadena positiva, sin una estructura CAP en su extremo 5´no traducido (5`UTR), pero con una proteína viral unida al genoma (VPg) y una cola poli A en su extremo 3´UTR. Se ha sugerido que proteínas del hospedero hacen un puente entre las regiones no traducidas virales 5´y 3´ para potenciar la traducción viral. Dado que las regiones no traducidas presentes en los genomas virales contienen elementos involucrados en la regulación de su ciclo replicativo, es importante analizar la interacción entre estas regiones y las proteínas virales o del hospedero para inferir su función. Objetivo: Determinar si existen proteínas del hospedero maíz que pudieran estar interactuando con la región 5´UTR de SCMV. Metodología: La región 5´UTR de SCMV se amplificó por PCR incluyendo la secuencia del promotor T7 en la secuencia del oligo 5´ UTR y se generaron sus transcritos marcados radiactivamente. Los transcritos marcados fueron entrecruzados con extractos proteicos de hojas de maíz sanas e infectadas. Resultados: Los resultados sugieren la presencia de una proteína putativa de interacción del hospedante maíz de aproximadamente 53kDa, con la región 5´UTR de SCMV. Conclusión: Los ensayos de entrecruzamiento, especificidad y estabilidad de los complejos ARN-proteína sugieren que en el hospedante maíz existe al menos una proteína que interactúa con la región 5´ UTR de SCMV.Palabras Clave: Complejo ARN-proteína, entrecruzamiento UV, fracción proteica, Potyvirus, VPg.Abstract Background: Sugarcane mosaic virus is a member of the great family of positive sense RNA viruses, without a CAP structure in its 5´UTR end, but with a viral protein attached to the genome (VPg) and a poli A tail in its 3´UTR end. It has been suggested that some host proteins make a bridge between the untranslated 5´and 3´ regions (UTRs) to enhance the canonical and/or non-canonical virus translation. Since the UTR regions present in the viral genomes have some elements involved in the regulation of their replicative cycle, it is important to analyze the interaction that may occur between these regions and the viral or host proteins to infer regarding its function. Objective: To identify proteins of the host maize that could be interacting with the 5´UTR region of SCMV. Methodology: 5´UTR region of SCMV was amplified by PCR including the sequence of the T7 promoter in the 5´oligo sequence. The transcripts were radioactively labeled. Labeled transcripts were crosslinked with proteins extracts from healthy and infected maize leaves. Results: The results suggest the presence of a 53 kDa putative protein interacting with the 5 ´UTR region of SCMV. Conclusion: Crosslinking essays, specificity and the stability of the RNA-protein complex suggest that in maize host there is at least one protein that interacts with the 5´UTR region of SCMV.Keywords: RNA-Protein complex, UV crosslinking, protein fraction, Potyvirus, VPg

show abstract

Virus and Host Plant Interactions

Wang

2018

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Plant viruses infect almost all crops and cause serious diseases worldwide. Currently, viral pathogens account for the largest proportion of newly emerging plant diseases and as such, are considered a major constraint to agriculture, threatening global food security. All plant viruses have relatively small genomes with limited coding capacity. They must co‐opt cellular pathways and recruit host proteins and metabolites to complete their infection cycle. To combat virus infection, plants have evolved sophisticated defence mechanisms. In order to establish infection, viruses have also evolved virulence strategies to suppress host defence. A successful infection by a plant virus requires compatible molecular interplays between the host plant and the invading virus. A better understanding of the complex virus–plant interactions will assist in the development of novel antiviral strategies. Key Concepts Plant viruses have small genomes that encode a few proteins and thus depend on host factors to establish their infection. There exist multifaceted defence and counterdefence strategies in the coevolutionary arms race between plants and viruses. A successful viral infection may induce disease symptoms or remain asymptomatic. Host factors are implicated in all steps of the viral infection process, and silencing or mutation of host factor gene(s) leads to recessive genetic resistance to viruses. The majority host factors are also essential for plant viability and some of them may be manipulated to develop genetic resistance through precise mutation to retain their canonical cellular functions but lose the ability to support viral infection.

show abstract

HC-Pro protein of sugar cane mosaic virus interacts specifically with maize ferredoxin-5 in vitro and in planta

Cited by 91 publications

References 38 publications

A Violaxanthin Deepoxidase Interacts with a Viral Suppressor of RNA Silencing to Inhibit Virus Amplification

A Violaxanthin Deepoxidase Interacts with a Viral Suppressor of RNA Silencing to Inhibit Virus Amplification

Exploración de la interacción entre la región 5´UTR del Sugarcane Mosaic Virus (SCMV) y proteínas del hospedero maíz

Virus and Host Plant Interactions

Contact Info

Product

Resources

About