HDL-binding lipoproteins on human fetal hepatocytes

Abstract

Two types of binding sites for high-density lipoproteins (Ud=l ~tg/ml and 20 gg/ml) were identified on human fetal hepatocytes and designated as P~ and P:. Ligand blotting has shown that P1 site is protein with Mr 100 kD and P2 site involves two proteins with Mr 105 and 110 kD. P2 proteins were not detected when incubation with ligands was shortened from 24 h to 30 mill. Molecular weight and activity of P~ did not change considerably after reduction of disulfide bonds.