HDL is the major lipoprotein carrier of plasma F2-isoprostanes

Abstract

Enhanced oxidative stress is implicated in the development of atherosclerosis in humans and animal models.

Impaired Fasting Glucose & 8-Iso-Prostaglandin F2α in Diabetes Disease Progression

“…Changes in serum lipids in type 2 diabetes have also been demonstrated in the IFG stage and are associated with oxidation of arachidonic acid to 8-iso-PGF 2α [42,43,37]. In our current study there was a significant reduction in both total cholesterol and HDL-C, which explains the increased 8-iso-PGF 2α as HDL-C carries 8-iso-PGF 2α [11]. Of importance is that no significant change in serum lipids in response to the statin use was noted.…”

“…These pathophysiological changes are in turn linked with lipid peroxidation, oxidative stress, and inflammation seen in IFG and diabetes [9]. Lipid peroxidation is most often measured using malondialdehyde (MDA) and 8-iso-prostaglandin F 2α (8-iso-PGF 2α ) [10][11][12]. Oxidative stress can be determined by GSH and 8-hydroxy-2-deoxyguanosine (8-OHdG) assays [13][14][15].…”

Functionality of HDL: Antioxidation and Detoxifying Effects

“…They can accumulate in the particle when its LOOH-inactivating capacity is overwhelmed. Furthermore, HDL represents a major plasma carrier of F2-isoprostanes, stable final products of lipid peroxidation (Proudfoot et al 2009). The ability of HDL to remove LOOH from cell membranes of erythrocytes and astrocytes has also been reported (Ferretti et al 2003;Klimov et al 2001).…”

Lomitapide affects HDL composition and function

“…Lipoprotein profiles were generated with density-gradient ultracentrifugation using the method described by Proudfoot et.al 11 . Lipoproteins were separated according to their densities into HDL3 (1·125-1·21 g/ml), HDL2 (1·062-1·125 g/ml), LDL(1·019-1·063 g/ml), and IDL+VLDL (<1·019 g/ml) 12 .…”