Head and neck cutaneous leishmania: clinical characteristics, microscopic features and molecular analysis in a cohort of 168 cases

Dunya, Gabriel; Habib, Robert H.; Moukarbel, Roger V; Khalifeh, Ibrahim

doi:10.1007/s00405-016-3978-5

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Early investigations agreed that PCR coupled with the conventional Giemsa staining test demonstrated better sensitivity of 74% with better outcomes in comparison to direct microscopy only (18.19,20,6). In contrast to some studies a study from Sindh show more female patients than male, our study and some of the other studies had similar finding (2122, 23,24). This may be due to demographic variables and the exposure behaviors that both sexes are likely to adopt.…”

Section: Discussioncontrasting

confidence: 67%

“…Face was the prominent area of bite as higher frequency noted with 48%, followed by 45% in upper extremities and 23% of lesions on lower extremities. This type of finding seen in other repots also (24,25). Due to outside activities and higher probability of exposure to the environmental factors young age groups are at higher risk of CL as reported commonly (26,27).…”

Section: Discussionsupporting

confidence: 57%

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PCR Based Detection of Cutaneous Leishmaniasis

Hakim¹,

Shah²,

Khan³

et al. 2023

PJMHS

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Background: The second most common illness after malaria that is transmitted by vectors and that may be lethal if untreated is leishmaniasis. Leishmaniasis is caused by parasites of the genus Leishmania, which are classified into two subgenera, Viannia and Leishmania. Methodology: A total of 100 sample collected from presumed clinically as CL patients by dermatologist, with 50 positive cases and 50 negative cases on Geimsa stain microscopy for Leishman Donovan (LD) bodies. After permission from Ethical board in Khyber medical university KPK and inform consent from patient or their guardian in case of children was taken. The lesion and its surroundings were cleaned with 70% ethanol, and the fluid oozing out of the first prick was obtained and disperse over a glass slide, left to dry and fixed with methanol, and stained with Giemsa stain. Two pricks were administered with a sterile BD syringe at the active margin of the lesion. The second prick's fluid was collected and kept in an EDTA tube for PCR usage at -20°C at the IBMS laboratory of KMU in Peshawar. Results: Out of 100 CL patients, 53 were female and 47 were male. The patients age was ranged from two year to 89 year. PCR was positive in 64 cases and negative in 36 cases out 100 for CL. Out of 50 positive microscopic cases 37cases were positive on PCR. Out of 50 microscopic negative cases 27 (54%) were positive by PCR. A statistically significant difference was found between PCR and microscopic method in the detection of CL. Conclusion: The finding in our study specifies that PCR base analysis is superior to microscopy for diagnosis of CL. Therefore, we recommend use of PCR for the detection of CL along with Giemsa staining in order to improve the quality and sensitivity of routine diagnosis of the disease. Keywords: Polymerase Chain Reaction, Giemsa staining, Cutaneous leishmaniasis

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Section: Discussioncontrasting

confidence: 67%

Section: Discussionsupporting

confidence: 57%

PCR Based Detection of Cutaneous Leishmaniasis

Hakim¹,

Shah²,

Khan³

et al. 2023

PJMHS

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Clinical, microscopic and molecular presentation in pediatric versus adult old world cutaneous Leishmaniasis

Dunya

Loya

Taraif

et al. 2020

Pediatric Dermatology

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BackgroundCutaneous leishmaniasis (CL) is an emerging uncontrolled tropical parasitic disease in endemic and nonendemic areas with a high prevalence in the pediatric age group.MethodA total of 382 individuals from Lebanon, Saudi Arabia, Pakistan, and Syria diagnosed with CL by punch biopsy/scrapings were grouped into adults (>18 years) and pediatrics (≤18 years). Data recorded included clinical features [number, location, type, size, and extensiveness (size larger than 3 cm, more than 5 lesions per patient, lesion present for more than 12 months, special types, disfiguring lesion or closeness to vital sensory organs) of lesions] and microscopic findings [Ridley's Parasitic Index and Ridley's Pattern]. In addition, molecular confirmation and speciation were performed.ResultsIn comparison with adults, patients in the pediatric group (n = 158, 41.4%) showed significantly higher number of lesions, more facial involvement, and more extensive disease (P < .05). Microscopically, a more advanced Ridley's pattern was observed. The other variables did not show statistical difference between the two groups.ConclusionHistorically, CL has been known to be a neglected tropical disease of poverty and pediatric predilection. In our pediatric group, CL manifests with more extensive disease clinically mirrored by more advanced lesions microscopically.

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