Headspace analysis of the volatile constituents from Juniperus communis L. ‘berries’ (cones) grown wild in Greece

Abstract

The fraction of highly volatile compounds is quite important to define the first sensory impact of an aromatic smell, and one of the most accurate ways to examine it is almost always by the headspace technique. A panel of 20 characteristic aromatic constituents, also shown to be the main constituents, has been studied in relation to juniper aroma. The results from the static headspace-GC (SH-GC) technique applied for the extraction and analysis of the volatile components from Juniperus communis L. berries (cones) showed that: (a) the monoterpene hydrocarbons formed the main group of constituents (73.5-93.7%); (b) temperatures and extraction times were significant factors, influencing the amounts of all the groups of volatiles; and (c) comminution of the plant material influenced the headspace procedure differently. Remarkable differences were observed, particularly between the quantitative synthesis of the distillate and the headspace extract.

Zimbro (Juniperus communis L.) as a Promising Source of Bioactive Compounds and Biomedical Activities: A Review on Recent Trends

“…communis , the main VOCs found in their essential oils were α -pinene (41.60%), β -pinene (27.60%), limonene (6.40%), β -myrcene (5.70%), and trans -pinocarveol (1.90%) [ 81 ]. A similar profile was found on Greek oil berries [ 82 ]. On the other hand, Iranian J. communis oils from the same species showed lower percentages of α -pinene (19.90%), and higher content of sabinene (36.8%) and limonene (10.60%) [ 83 ].…”

“…On the other hand, in Juniper oil berry var. alpina from Virginia (USA), α -pinene was the predominant one (20.00%), followed by δ-cadinene (10.40%), limonene and β -myrcene (8.70 and 8.50%, respectively) and borneol (8.00%) [ 82 ]. a -Pinene was also the main reported in var.…”

Chemical Composition, Cytotoxic and Antibacterial Activities of Essential Oils of Cultivated Clones of Juniperus communis and Wild Juniperus Species

“…communis growing at lower altitude, headspace GC/MS experiment was also conducted, 5 g each fresh needles were poured into three 20 ml headspace vials, and then immediately sealed with an aluminum cap having silicon rubber septa. The vials were then transferred to head space tray and analyzed with Shimadzu headspace, auto‐injector AOC‐5000 connected to Shimadzu GC/MS . The incubation temperature of vials was 110 °C and maintained the incubation time for 5 (first vial), 15 (second vial) and 25 (third vial) min, respectively, with an agitation at 500 rpm.…”

“…The vials were then transferred to head space tray and analyzed with Shimadzu headspace, autoinjector AOC-5000 connected to Shimadzu GC/MS. [62] The incubation temperature of vials was 110°C and maintained the incubation time for 5 (first vial), 15 (second vial) and 25 (third vial) min, respectively, with an agitation at 500 rpm. The temperature of sampling needle was 125°C.…”

Seasonal variation in phytochemicals of essential oil fromJuniperus communisneedles in western Himalaya

“…Figure 1 shows the typical GC chromatograms of essential oil components observed in spring, rainy and winter seasons. Earlier reports on analysis of essential oil composition shows the presence of main components as α-pinene, sabinene, limonene, terpinen-4-ol, trans-sabinyl acetate, α-thujene in J. communis and J. wallichiana (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). Our research also counts the presence of sabinene, limonene, 4-terpineol, α-pinene, cis-sabinene hydrate, α-cadinol, myrcene, α-terpinene and manool as major components.…”