Healing activity of proteolytic fraction (P1G10) from Vasconcellea cundinamarcensis in a cutaneous wound excision model.

Freitas, Kátia Michelle; Barcelos, Lucíola da Silva; Caliari, Marcelo Vidigal; Salas, Carlos; Lopes, Míriam Teresa Paz

doi:10.1016/j.biopha.2017.09.109

Cited by 12 publications

(10 citation statements)

References 40 publications

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“…Commonly used in the pharmaceutical and cosmetic industries, plant extracts with antioxidant properties decrease the production of ROS. Various traditional plant extracts have well-known properties, including medicinal functions, pharmacological activities, and skin-protective effects [9], [10]. The efficacy of ginseng, the representative product of Korea, and its chemical effects have been well investigated.…”

Section: Introductionmentioning

confidence: 99%

A new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts

Lee

Hong

Tran

et al. 2019

Journal of Ginseng Research

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Background The efficacy of ginseng, the representative product of Korea, and its chemical effects have been well investigated. The ginsenoside RG3 has been reported to exhibit apoptotic, anticancer, and antidepressant-like effects. Methods In this report, the putative effect of RG3 on several cellular function including cell survival, differentiation, development and aging process were evaluated by monitoring each specific marker. Also, mitochondrial morphology and function were investigated in ultraviolet (UV)-irradiated normal human dermal fibroblast cells. Results RG3 treatment increased the expression of extracellular matrix proteins, growth-associated immediate-early genes, and cell proliferation genes in UV-irradiated normal human dermal fibroblast cells. And, RG3 also resulted in enhanced expression of antioxidant proteins such as nuclear factor erythroid 2–related factor-2 and heme oxygenase-1. In addition, RG3 affects the morphology of UV-induced mitochondria and plays a role in protecting mitochondrial dysfunction. Conclusioin RG3 restores mitochondrial adenosine triphosphate (ATP) and membrane potential via its antioxidant effects in skin cells damaged by UV irradiation, leading to an increase in proteins linked with the extracellular matrix, cell proliferation, and antioxidant activity.

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Section: Introductionmentioning

confidence: 99%

A new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts

Lee

Hong

Tran

et al. 2019

Journal of Ginseng Research

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“…2b-c and 3a,b). In excisional lesions, P1G10 reduced its healing capacity when the proteolytic activity was inhibited 8 . Similarly, oral administration of active bromelain was required for efficacy as anti-inflammatory 37,38 .…”

Section: Discussionmentioning

confidence: 98%

“…P1G10 displays gastric protective and healing activities by increasing mucus content and PGE2 secretion, in indomethacin and acetic acid induced rodent lesions 6 . P1G10 also reduced gastrin levels, preventing hyperacidity and gastric damage 7 and improved repair in excisional wounds, increasing the lesion resolution and modulating inflammatory mediators 8 . In this excisional model the proteolytic activity of P1G10 was required to enhance healing, while it was not relevant for gastroprotective action 7 .…”

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confidence: 99%

The Proteolytic Fraction From Vasconcellea cundinamarcensis Latex Displays Anti-Inflammatory Effect in A Mouse Model of Acute TNBS-Induced Colitis

Albuquerque

Pizzitola

Silva

et al. 2020

Sci Rep

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The proteolytic fraction (P1G10) from Vasconcellea cundinamarcensis, displays gastric protective and healing activities in different skin lesions in mice and human. In an excisional model, this fraction accelerates resolution of lesions and modulates inflammatory mediators. Based on these data, we assessed its anti-inflammatory activity in murine colitis model, induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) adopted by its physiopathological similarity with human colitis. Twenty four hours after colitis induction followed by three days of treatment, P1G10 at 0.3 and 3.0 mg/Kg induced 30% increase in body weight (p < 0.0001) and ~80% reduction in colon macroscopic damage score (p < 0.05) compared to the untreated TNBS-induced colitis group. Histological analyses showed that 0.3 mg/Kg P1G10 reduced the inflammatory profile and tissue damage (47%, p < 0.05) when it was proteolytically active. Compared to TNBS group, 0.3 mg/Kg P1G10 reduced MPO activity (80%, p < 0.01), MCP-1 (47%, p < 0.05) and TNF-α (50%, no significant) and increased IL-10 (330%, p < 0.001) levels in the supernatant of colonic tissue homogenate. P1G10 treatment also reduced COX-2 expression (60%, p < 0.05) and metalloprotease-2 activity (39%, p < 0.05) while increased globet cell density (140%, p < 0.01), that contributes to mucus layer protection in colonic tissue. Taken together, these findings suggest that low doses of active P1G10 promotes lesion resolution, at least in part by its anti-inflammatory activity, in TNBS-colitis model.

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“…The rationale for analyzing the topical effect of P1G10 in UV lesions is based on reports showing that P1G10 accelerates wound healing and downregulates inflammatory symptoms within a shorter interval [30,31]. Figure 3A,B summarizes the changes in erythema and skin thickness induced by P1G10 in irradiated skin at 24 h.…”

Section: Discussionmentioning

confidence: 99%

P1G10, the Proteolytic Fraction from Vasconcellea cundinamarcensis, Stimulates Tissue Repair after Acute Exposure to Ultraviolet B Radiation

Freitas

Silva

Veloso

et al. 2019

IJMS

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Background: P1G10 is a cysteine proteolytic fraction from Vasconcellea cundinamarcensis latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was suggested an antioxidant role. Methods: We evaluated here the effect of topical treatment with P1G10, in mice lesions induced by UVB. Results: After single exposure to 2.4 J cm−2 UVB, P1G10 reduced erythema, increased cellularity of hypodermis, enhanced MPO activity and IL1β, and inhibited COX2 levels. These results point to an anti-inflammatory effect by P1G10. This fraction displayed antioxidant activity by reversing the depletion of glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and reducing the catalase activity increased by UVB. These changes may be related to a reduction in MDA observed in groups treated with P1G10. P1G10 also inhibited MMP-9, caspase-3 and pkat while increasing p53 levels.

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Healing activity of proteolytic fraction (P1G10) from Vasconcellea cundinamarcensis in a cutaneous wound excision model.

Cited by 12 publications

References 40 publications

A new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts

A new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts

The Proteolytic Fraction From Vasconcellea cundinamarcensis Latex Displays Anti-Inflammatory Effect in A Mouse Model of Acute TNBS-Induced Colitis

P1G10, the Proteolytic Fraction from Vasconcellea cundinamarcensis, Stimulates Tissue Repair after Acute Exposure to Ultraviolet B Radiation

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