Healing of Chronic Wounds: An Update of Recent Developments and Future Possibilities

Kathawala, Mustafa Hussain; Ng, Wei Long; Liú, Dan; Naing, May Win; Yeong, Wai Yee; Spiller, Kara L.; Dyke, Mark Van; Ng, Kee Woei

doi:10.1089/ten.teb.2019.0019

Cited by 81 publications

(51 citation statements)

References 174 publications

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“…[ 6 ] Nonetheless, protein‐based materials of animal origin introduce the risk of xenogeneic infections and adverse immune response. [ 7 ] Moreover, large‐scale production of proteinaceous scaffolds poses an economic conundrum due to limited natural protein sources and the high cost of producing recombinant proteins as a substitute. To address these concerns, a promising solution is to exploit human hair waste as an abundant and renewable source of keratin proteins to develop an autologous biological scaffold.…”

Section: Figurementioning

confidence: 99%

“…[ 12 ] Specific to dermal wound healing applications, interconnected porosity enables moisture retention, absorption of wound exudates, and gaseous exchange while the 3D structure provides mechanical integrity for dermis restoration. [ 7,13 ] For instance, Wang et al. reported that HHK hydrogels encapsulated with L929 fibroblasts could perform comparably well, relative to collagen hydrogels, in terms of supporting cell proliferation and were better at resisting graft contraction.…”

Section: Figurementioning

confidence: 99%

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Cryogelation of Human Hair Keratins

Chua

Zhao

2020

Macromol. Rapid Commun.

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Human hair keratins (HHK) are known for their biocompatibility and potential to regulate cell response, possibly due to the presence of the leucine‐aspartic‐valine cell adhesion and signaling motifs. Together with the abundance of cysteine residues in HHK, 3D HHK scaffolds are fabricated through cryogelation based on spontaneous disulfide crosslinks and noncovalent interactions. Herein, the molecular mechanism of HHK self‐assembly during cryogelation is interrogated and the influence of cryogelation parameters on the properties of the resultant scaffolds is studied. With successive freeze–thaw cycles, the storage modulus (G′) of HHK cryogels substantially improves from 116.4 Pa at freeze–thaw cycle 3 (FT3) to 1908.7 Pa at freeze–thaw cycle 10 (FT10). Meanwhile, it is found that complete thiol‐capping of HHK samples significantly inhibits cryogel formation as compared to partially or uncapped HHK samples, suggesting the dominant role of disulfide stabilization in cryogelation. Finally, uniaxial compression tests on HHK sponges demonstrate that FT cycling, from 0 to 10, is able to improve the compression modulus of sponges by ≈12‐folds. These findings show that macroscale properties of HHK cryogels can be conveniently modulated by physical parameters of cryogelation and that disulfide bonding is the main stabilizing force in HHK cryogels.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Cryogelation of Human Hair Keratins

Chua

Zhao

2020

Macromol. Rapid Commun.

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“…Collagen type I-based hydrogels are the most used substrate when producing skin equivalents (Kim B. S. et al, 2018). A wide range of products and strategies are now available for purposes of skin repair and grafting, toxicology testing and drug development (Shevchenko et al, 2010;Kathawala et al, 2019). Composite hydrogels are growing in popularity for skin regeneration because the native skin matrix is an orchestrated blend of several ECM components including collagen, elastin, fibronectin, vitronectin, glycosaminoglycans, and more.…”

Section: Skinmentioning

confidence: 99%

Composite Hydrogels in Three-Dimensional in vitro Models

Zhao

Vizetto‐Duarte

Moay

et al. 2020

Front. Bioeng. Biotechnol.

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3-dimensional (3D) in vitro models were developed in order to mimic the complexity of real organ/tissue in a dish. They offer new possibilities to model biological processes in more physiologically relevant ways which can be applied to a myriad of applications including drug development, toxicity screening and regenerative medicine. Hydrogels are the most relevant tissue-like matrices to support the development of 3D in vitro models since they are in many ways akin to the native extracellular matrix (ECM). For the purpose of further improving matrix relevance or to impart specific functionalities, composite hydrogels have attracted increasing attention. These could incorporate drugs to control cell fates, additional ECM elements to improve mechanical properties, biomolecules to improve biological activities or any combinations of the above. In this Review, recent developments in using composite hydrogels laden with cells as biomimetic tissue-or organ-like constructs, and as matrices for multi-cell type organoid cultures are highlighted. The latest composite hydrogel systems that contain nanomaterials, biological factors, and combinations of biopolymers (e.g., proteins and polysaccharide), such as Interpenetrating Networks (IPNs) and Soft Network Composites (SNCs) are also presented. While promising, challenges remain. These will be discussed in light of future perspectives toward encompassing diverse composite hydrogel platforms for an improved organ environment in vitro.

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“…[ 1–2 ] During the long‐time process of skin repair, wound healing is easily interrupted and hindered by some incidents, which may decrease the regenerative effects and disturb the tissue repair. [ 3 ] Particularly on diabetic patients, some chemokines and growth factors irregulating cell functions are extremely constrained to cause a worse recovery. [ 4–5 ] Consequently, skin injury is still a major healthcare problem, especially for the patients with chronic ulcers.…”

Section: Introductionmentioning

confidence: 99%

High Flexible and Broad Antibacterial Nanodressing Induces Complete Skin Repair with Angiogenic and Follicle Regeneration

Wang

Zha

et al. 2020

Adv Healthcare Materials

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Complete skin reconstruction is a hierarchically physiological assembly involving epidermis, dermis, vasculature, innervation, hair follicles, and sweat glands. Despite various wound dressings having been developed for skin regeneration, few works refer to the complete skin regeneration, particularly lacking for vasculatures and hair follicles. Herein, an instructive wound dressing that integrates the antibacterial property of quaternized chitin and the mechanical strength and biological multifunction of silk fibroin through layer-by-layer electrostatic self-assembly is designed and reported. The resultant dressings exhibit a nanofibrous structure ranging from 471.5 ± 212.1 to 756.9 ± 241.8 nm, suitable flexibility with tensile strength up to 4.47 ± 0.29 MPa, and broad-spectrum antibacterial activity against Escherichia coli and Staphylococcus aureus. More interestingly, the current dressing system remarkably accelerates in vivo vascular reconstruction within 15 days, and the number of regenerated hair follicles reaches up to 22 ± 4 mm −2 , which is comparable to the normal tissue (27 ± 2 mm −2). Those crucial functions might originate from the combination between quaternized chitin and silk fibroin and the hierarchical structure of electrospun nanofiber. This work establishes an easy but effective pathway to design a multifunctional wound dressing for the complete skin regeneration.

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Healing of Chronic Wounds: An Update of Recent Developments and Future Possibilities

Abstract: Healing of chronic wounds : an update of recent developments and future possibilities Kathawala,

Cited by 81 publications

References 174 publications

Cryogelation of Human Hair Keratins

Cryogelation of Human Hair Keratins

Composite Hydrogels in Three-Dimensional in vitro Models

High Flexible and Broad Antibacterial Nanodressing Induces Complete Skin Repair with Angiogenic and Follicle Regeneration

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