Healing of Excimer Laser Ablated Monkey Corneas

SundarRaj, Nirmala; Geiss, Michael J.; Fantes, Francisco; Hanna, Khalil; Anderson, Susan C.; Thompson, Keith P.; Thoft, Richard A.; Waring, George O.

doi:10.1001/archopht.1990.01070130106039

Cited by 163 publications

(32 citation statements)

References 27 publications

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“…Radial keratotomy (RK) procedures were performed by one of us (GM), in seven male monkeys (Macaca mulatta), [5][6][7] kg in weight, aged 7-8 years. All animals were housed and treated according to the Association for Research in Vision and Ophthalmology resolution for animal care.…”

Section: Methodsmentioning

confidence: 99%

“…After marking a 3.0 mm optical clear zone (OCZ) centered understanding of how the extracellular matrix is organized on the pupil (Moria Instruments, Paris, France), centripetal within the scan incisions were made using the perpendicular cut edge, with Although conflicting reports have described its presence or the eye stabilized with a toothed forceps at the opposite limbus. absence in the adult corneal stroma (6,18,19,20,21,22), All monkeys underwent a bilateral, six incision RK procedtype III collagen may be present in the fetal and regenerating ure; three semi-radial incisions were placed in each half of the cornea (23,24,25), By its interaction with fibronectin and type I collagen, type III collagen may have a role in controlling early fibrillogenesis (20,26) (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

“…Following wounding, fibroblasts express an intermediate filament-associated protein (IFAP 130) (36), that is not detected in quiescent cells. IFAP 130 may therefore be used to identify activated fibroblasts, and to compare the duration of their activated condition in healing wounds (5,6).…”

Section: Introductionmentioning

confidence: 99%

“…To determine how the former presence of an epithelial plug affects the stromal healing response, unsutured and sutured keratotomy wounds were analyzed by immunolabeling for fibronectin, type III, type VI and type VII collagen, KS and IFAP 130, of the incisions, were transferred to glass slides and immunoreacted with specific antibodies at appropriate dilutions using an indirect fluorescein isothiocyanate (FITC)-conjugated antibody technique (6). Tissue sections on the slides were reacted successively with 5% heat-inactivated rabbit serum in phosphate-buffered saline for 45 min, the appropriate antibody (10…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical analysis of unsutured and sutured corneal wound healing

Melles

SundarRaj

Binder³

et al. 1995

Current Eye Research

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In the unsutured partial thickness penetrating wounds of the regions. In sutured wounds, effective wound repair may be cornea, the epithelium migrates over the wounded stromal established from the anterior to posterior regions, after a surface prior to the onset of stromal regeneration. To determine relatively long period of a disorganized state of the scar tissues, the possible affects of the epithelial ingrowth on the organiza tion of the stromal scar tissues, the healing of unsutured and sutured wounds was compared immunohistochemically. Immunostaining patterns for fibronectin, types III, VI and VII These observations suggest that the temporary presence of an epithelial plug within an unsutured wound and/or wound gaping induce a different stromal healing response from that in the sutured wounds, that results in an eventual ineffective collagen, keratan sulfate proteoglycan (KSPG), and interwound repair. Curr. Eye Res. 14: 809-817, 1995. mediate filament-associated protein (IFAP 130) in fibroblasts, were analyzed in unsutured and adjacent sutured keratotomy w ounds in monkeys, at 2-9 weeks after surgery. At 2-4 weeks, fibronectin, type III and type VI collagen showed a lamellar interweaving pattern across unsutured wounds that was absent in sutured wounds. Type VII collagen was detected along the entire depth of regenerated stroma in unsutured wounds, but not in sutured wounds indicating that the epithelium had

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical analysis of unsutured and sutured corneal wound healing

Melles

SundarRaj

Binder³

et al. 1995

Current Eye Research

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“…18,62,63 Histological studies of excimer laser ablated monkey corneas have shown increased amounts of type 7 collagen, a major component of anchoring fibrils and hemidesmosomes along the basement membrane of the basal epithelial cells. 63,64 Human studies have shown that the basal epithelial layer forms hemidesmosomes and new basement membrane within 2 weeks of photoablation. 65 In addition, undulations are most noticeable on the edge of the ablated zone, which would greatly increase the surface area of attachment.…”

Section: Anterior Stromal Puncturementioning

confidence: 99%

Pathogenesis, clinical features and management of recurrent corneal erosions

Ramamurthi

Rahman

Dutton

et al. 2005

Eye

111

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Recurrent corneal erosions (RCE) are common. They are characterised by repeated episodes of pain, difficulty in opening the eyes, watering, and photophobia resulting from poor epithelial adhesion. In the majority of patients with RCE, trauma is the initiating factor. Epithelial, stromal, and endothelial corneal dystrophies have all been described in association with RCE. Other causes that may lead to RCE include chemical and thermal injuries, previous herpetic keratitis, meibomian gland dysfunction, ocular rosacea, diabetes mellitus, Salzmann's nodular degeneration, band keratopathy, previous bacterial ulceration, kerato-conjunctivitis sicca, and epidermolysis bullosa. The conditions that are associated with RCE can be either primary or secondary depending on whether the basement membrane complex abnormality is intrinsic or acquired. Primary types tend to be bilateral, symmetrical and develop in multiple corneal locations. The pathogenetic mechanism of this disorder is related to poor adhesion of the corneal epithelium to the underlying stroma. Excessive matrix metalloproteinase (MMP) activity may play a role in the pathogenesis. Although the majority of patients will respond to simple measures such as padding and antibiotic ointment, RCE resistant to simple measures require approaches that are more elaborate. The common goal of these approaches is to encourage proper formation of adhesion complexes between the epithelium and the stroma. The use of longterm contact lenses, autologous serum eye drops, botulinum toxin, induced ptosis, oral MMP inhibitors, diamond burr polishing of Bowman's membrane have been reported with varying degree of success in treating RCE. Anterior stromal puncture with insulin needles or Neodymium : aluminium-yttriumgarnet may enhance the epithelial adhesion to the basement membrane by scar formation and success rates of up to 80% have been reported in the treatment of recalcitrant RCE. Excimer laser photo-therapeutic keratectomy (PTK) is now a well-established treatment modality for RCE and is being used both safely and effectively. Partial ablation of Bowman's layer with PTK gives a smooth surface for the newly generating epithelium to migrate and form adhesion complexes. The pathogenesis, clinical features, and management options of this common disorder are discussed in this review article.

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Phototherapeutic Keratectomy for Anterior Basement Membrane Dystrophy After Laser In Situ Keratomileusis

Rojas

Manche²

2002

Arch Ophthalmol

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To evaluate the efficacy and safety of phototherapeutic keratectomy (PTK) in the treatment of symptomatic anterior basement membrane dystrophy following laser in situ keratomileusis (LASIK). Methods: In a retrospective study, 10 eyes of 10 patients that developed symptomatic anterior basement membrane dystrophy following LASIK for myopia were treated with PTK using the VISX S2 (VISX Inc, Santa Clara, Calif) excimer laser. Primary outcome measurements including corneal clarity, resolution of symptoms, uncorrected visual acuity (UCVA), manifest refraction, best spectacle-corrected visual acuity (BSCVA), and complications were evaluated preoperatively, 1 day postoperatively, and at the last postoperative follow-up visit. Results: At the last follow-up visit (mean [SD], 8.8 [5.5] months; range, 4-22 months), 100% of the eyes had clear corneas with no evidence of anterior basement membrane dystrophy, and all eyes were asymptomatic. Mean spherical equivalent changed from −0.75 (0.99) diop-(REPRINTED) ARCH OPHTHALMOL / VOL 120, JUNE 2002 WWW.ARCHOPHTHALMOL.COM

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Healing of Excimer Laser Ablated Monkey Corneas

Cited by 163 publications

References 27 publications

Immunohistochemical analysis of unsutured and sutured corneal wound healing

Immunohistochemical analysis of unsutured and sutured corneal wound healing

Pathogenesis, clinical features and management of recurrent corneal erosions

Phototherapeutic Keratectomy for Anterior Basement Membrane Dystrophy After Laser In Situ Keratomileusis

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