Health and performance of young dairy calves vaccinated with a modified-live Mannheimia haemolytica and Pasteurella multocida vaccine

Aubry, P.; Warnick, Lorin D.; Guard, Chuck; Hill, Bruce W.; Witt, Michael F.

doi:10.2460/javma.2001.219.1739

Cited by 21 publications

(10 citation statements)

References 13 publications

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“…[8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] One or more trials from five other studies were identified that utilized feedlot cattle in typical North American production settings, but they were weakened by lack of blinding, treatment being confounded by arrival group or other vaccine treatment, or significant loss-to-follow up and were discarded from the summary. [23][24][25][26][27] In addition, three terminal studies (five trials) investigated the use of commercially available vaccines in feedlot cattle with a pathogen-challenged disease model [14,28,29], three studies (five trials) utilized dairy or beef calves with naturally occurring disease to investigate effects of vaccination [27,30,31], and thirteen studies investigated the use of commercially available vaccines in dairy calves with an induced-disease model. [32][33][34][35][36][37][38][39][40][41][42][43][44] Studies were excluded from the review: if they did not report original data (primary study), if they did not include a non-vaccinated/placebo control group, if the outcome did not include an assessment of morbidity risk, mortality risk, or extent of lung involvement (e.g.…”

Section: Methodsmentioning

confidence: 99%

Evidence-Based Effectiveness of Vaccination Against Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni in Feedlot Cattle for Mitigating the Incidence and Effect of Bovine Respiratory Disease Complex

Larson

Step

2012

Veterinary Clinics of North America: Food Animal Practice

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Section: Methodsmentioning

confidence: 99%

Evidence-Based Effectiveness of Vaccination Against Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni in Feedlot Cattle for Mitigating the Incidence and Effect of Bovine Respiratory Disease Complex

Larson

Step

2012

Veterinary Clinics of North America: Food Animal Practice

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“…These include live M. haemolytica (2,6,7,9,11,19,21,33,50), killed M. haemolytica cells (10,20,22,23,52), components or fractions of M. haemolytica cells (17,24,27,28,35,49,(53)(54)(55), and commercial vaccines (57,58). While immunity to M. haemolytica appears to require anti-LKT and antisurface antigen antibodies (55), partial protection could be afforded to cattle experimentally vaccinated with M. haemolytica preparations that did not contain LKT (21,40).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Immunodominant and Potentially Protective Epitopes ofMannheimia haemolyticaSerotype 1 Outer Membrane Lipoprotein PlpE

2004

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Mannheimia haemolytica serotype 1 (S1) is the most common bacterial isolate found in shipping fever pneumonia in beef cattle. Currently used vaccines against M. haemolytica do not provide complete protection against the disease. Research with M. haemolytica outer membrane proteins (OMPs) has shown that antibodies to one particular OMP from S1, PlpE, may be important in immunity. In a recently published work, members of our laboratory showed that recombinant PlpE (rPlpE) is highly immunogenic when injected subcutaneously into cattle and that the acquired immunity markedly enhanced resistance to experimental challenge (A. W. Confer, S. Ayalew, R. J. Panciera, M. Montelongo, L. C. Whitworth, and J. D. Hammer, Vaccine 21:2821-2829, 2003). The objective of this work was to identify epitopes of PlpE that are responsible for inducing the immune response. Western blot analysis of a series of rPlpE with nested deletions on both termini with bovine anti-PlpE hyperimmune sera showed that the immunodominant region is located close to the N terminus of PlpE. Fine epitope mapping, in which an array of overlapping 13-mer synthetic peptides attached to a derivatized cellulose membrane was probed with various affinity-purified anti-PlpE antibodies, identified eight highly reactive regions, of which region 2 (R2) was identified as the specific epitope. The R2 region is comprised of eight imperfect repeats of a hexapeptide (QAQNAP) and is located between residues 26 and 76. Complementmediated bactericidal activity of affinity-purified anti-PlpE bovine antibodies confirmed that antibodies directed against the R2 region are effective in killing M. haemolytica.Bovine respiratory disease arises from the interaction of numerous contributing factors, including physical stresses associated with weaning, shipment, inclement weather, and overcrowding coupled with viral and bacterial infections (8,31,32,59,63). The result in severe cases is colonization of the lungs with pathogenic bacteria resulting in severe pneumonia. Pasteurella multocida, Haemophilus somnus, and Mannheimia (formerly Pasteurella) haemolytica are associated with bovine pneumonia. However, M. haemolytica serotype 1 (S1) is by far the most important bacterial pathogen in the development of the often-fatal fibrinous pleuropneumonia in beef cattle known as pneumonic pasteurellosis or shipping fever (31, 32).Immunity against M. haemolytica is thought to be primarily through production of serum antibodies that neutralize the secreted leukotoxin (LKT) and antibodies against surface antigens (45). The mechanism of activity of antisurface antibodies and the specific surface antigens involved in anti-M. haemolytica immunity are not known; however, complementmediated bacterial lysis and bacterial phagocytosis and killing are thought to be important in defense against M. haemolytica infection (45). Complement-mediated bactericidal activity against M. haemolytica and phagocytosis of M. haemolytica by bovine neutrophils has been demonstrated with bovine immune serum (12,17,40,46).Litt...

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“…SBD-1 is developmentally regulated in late gestation through the neonatal period with maximal expression in trachea, lung and rumen reached shortly after birth [19 -21]. Although SBD-1 appears to lack NF-kappa B responsive elements, [21][22][23][24][25][26][27] www.elsevier.com/locate/micpath SBD-1 expression is increased during infection with ovine parainfluenza virus type 3 (PI-3) [22]. SBD-2 is also developmentally regulated and expressed primarily in the alimentary tract [20,23]; however, only low levels of SBD-2 mRNA are present in lung relative to SBD-1 [20,23].…”

Section: Introductionmentioning

confidence: 99%

“…M. haemolytica can cause serious outbreaks of acute pneumonia in neonates, weaned and growing animals as well as adults [24]. Vaccination against M. haemolytica can enhance resistance to infection; however, these vaccines are not completely effective [25,26]. Experimentally, M. haemolytica activates NF-kappa B signaling [15,28] and enhances expression of two inducible bovine defensins, TAP and LAP in cattle [16 -18].…”

Section: Introductionmentioning

confidence: 99%

Differential expression of sheep beta-defensin-1 and -2 and interleukin 8 during acute Mannheimia haemolytica pneumonia

Ackermann¹,

Gallup²,

Zabner³

et al. 2004

Microbial Pathogenesis

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Beta-defensins are antimicrobial peptides produced by several cell types, including respiratory epithelia and leukocytes. Expression of some beta-defensins is increased by bacterial-induced inflammatory responses whereas expression of other beta-defensins is constitutive. Two beta-defensins are expressed in lungs of sheep (sheep beta-defensin-1 and -2; SBD-1/-2) and expression of SBD-1 is increased during parainfluenza virus type 3 (PI-3) infection. The effect of Mannheimia haemolytica, a Gram-negative bacteria known to induce expression of bovine beta-defensins and NF-kappa B in lung, has not been determined for SBD-1/-2. In this study, different concentrations of M. haemolytica were inoculated into pulmonary bronchi of lambs. SBD-1 and SBD-2 mRNA levels detected by real time reverse transcriptase polymerase chain reaction in lung homogenates did not increase. In fact, SBD-1 mRNA levels were significantly decreased with the highest administered inoculum concentration (10 9 ). In contrast, mRNA levels of interleukin-8 (IL-8) were significantly increased over controls and progressively increased with M. haemolytica concentrations. Coinoculation of M. haemolytica with xylitol, an osmotic agent, did not alter mRNA levels of SBD-1, SBD-2 or IL-8. SBD-1 mRNA expression was detected in lung epithelia, but not in leukocytes. This study suggests that SDB-1 expression occurs in epithelia and decreases during severe bacterial pneumonia, which is in contrast to the increase that occurs with PI-3 infection. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. AuthorsMark R. Ackermann, Jack M. Abstract Beta-defensins are antimicrobial peptides produced by several cell types, including respiratory epithelia and leukocytes. Expression of some beta-defensins is increased by bacterial-induced inflammatory responses whereas expression of other beta-defensins is constitutive. Two beta-defensins are expressed in lungs of sheep (sheep beta-defensin-1 and -2; SBD-1/-2) and expression of SBD-1 is increased during parainfluenza virus type 3 (PI-3) infection. The effect of Mannheimia haemolytica, a Gram-negative bacteria known to induce expression of bovine beta-defensins and NF-kappa B in lung, has not been determined for SBD-1/-2. In this study, different concentrations of M. haemolytica were inoculated into pulmonary bronchi of lambs. SBD-1 and SBD-2 mRNA levels detected by real time reverse transcriptase polymerase chain reaction in lung homogenates did not increase. In fact, SBD-1 mRNA levels were significantly decreased with the highest administered inoculum concentration (10 9 ). In contrast, mRNA levels of interleukin-8 (IL-8) were significantly increased over controls and progressively increased with M. haemolytica concentrations. Co-inoculation of M. haemolytica with xylitol, an osmotic agent, did not alter mRNA levels of SBD-1, SBD-2 or IL-8. SBD-1 mRNA expression was detected in lung epithelia, b...

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Health and performance of young dairy calves vaccinated with a modified-live Mannheimia haemolytica and Pasteurella multocida vaccine

Abstract: Results suggest that the M. haemolytica and P. multocida vaccine, given twice 2 weeks apart, was effective in increasing titers of antibodies against M. haemolytica in young dairy calves but did not improve calf performance or health.

Cited by 21 publications

References 13 publications

Evidence-Based Effectiveness of Vaccination Against Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni in Feedlot Cattle for Mitigating the Incidence and Effect of Bovine Respiratory Disease Complex

Evidence-Based Effectiveness of Vaccination Against Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni in Feedlot Cattle for Mitigating the Incidence and Effect of Bovine Respiratory Disease Complex

Characterization of Immunodominant and Potentially Protective Epitopes ofMannheimia haemolyticaSerotype 1 Outer Membrane Lipoprotein PlpE

Differential expression of sheep beta-defensin-1 and -2 and interleukin 8 during acute Mannheimia haemolytica pneumonia

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