Health Benefits of Antioxidative Peptides Derived from Legume Proteins with a High Amino Acid Score

Matemu, Athanasia O.; Nakamura, Soichiro; Katayama, Shigeru

doi:10.3390/antiox10020316

Cited by 67 publications

(49 citation statements)

References 121 publications

(120 reference statements)

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“…Concerning the amino acid composition of bioactive peptides, the presence of hydrophobic amino acid residues, such as W, F, P, G, K, I, and V, at both the N-terminus and C-terminus, are relevant for peptide antioxidative activity, as well as H and R at the C-terminus position [ 43 ]. In particular, the presence of H has been associated with metal-ion chelator, active-oxygen quencher, and hydroxyl radical scavenger properties [ 4 , 7 ]. Another contribution to the antioxidative activity (by free radical quenching) is provided by the excess electrons of the negatively charged residues E and D. Also, the relative positions of these amino acids can determine or enhance their activity [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

“…Since the last century, soybean cultivation has become widespread in western countries, too [ 4 ]. Soy proteins contain all the essential amino acids, and therefore they represent a valid alternative to food of animal origin; furthermore, several biological functions and bioactivities have been attributed to their derived peptides [ 13 ], such as cancer and cardiovascular disease prevention, antihypertensive activity [ 14 ], hypocholesterolemic effect, and antioxidant properties [ 1 , 4 , 7 , 15 , 16 ]. In particular, the antioxidant activity, i.e., the defense against the free radical damage [ 17 ], could be useful in contrasting several pathologies, as well as in nutraceutical and cosmetics applications.…”

Section: Introductionmentioning

confidence: 99%

“…Since dietary proteins represent a cheap and valuable source of bioactive peptides, several foods of both animal and plant origin [2,6,7] have been investigated for this aim, including milk [3,[8][9][10], meat, fish [11,12], egg, cereals, and soybean [1,3,7,13].…”

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Medium-Sized and Short Antioxidant Peptides from Soy Flour-Simulated Gastrointestinal Hydrolysate

et al. 2021

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Soybeans (Glycine max) are an excellent source of dietary proteins and peptides with potential biological activities, such as antihypertensive, anti-cholesterol, and antioxidant activity; moreover, they could prevent cancer. Also, soy contains all the essential amino acids for nutrition; therefore, it represents an alternative to animal proteins. The goal of this paper was the comprehensive characterization of medium-sized and short peptides (two to four amino acids) obtained from simulated gastrointestinal digestion. Two different analytical approaches were employed for peptide characterization, namely a common peptidomic analysis for medium-sized peptides and a suspect screening analysis for short peptides, employing an inclusion list of exact m/z values of all possible amino acid combinations. Moreover, fractionation by preparative reversed-phase liquid chromatography was employed to simplify the starting protein hydrolysate. Six fractions were collected and tested for antioxidative activity by an innovative antioxidant assay on human gastric adenocarcinoma AGS cell lines. The two most active fractions (2 and 3) were then characterized by a peptidomic approach and database search, as well as by a suspect screening approach, in order to identify potential antioxidant amino acid sequences. Some of the peptides identified in these two fractions have been already reported in the literature for their antioxidant activity.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Medium-Sized and Short Antioxidant Peptides from Soy Flour-Simulated Gastrointestinal Hydrolysate

et al. 2021

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“…Alkaline phosphatase hydrolysates of soy protein, containing the peptides LLPLPVLK, SWLRL, and WLRL, exhibited antioxidant activity and inhibited α-glucosidase, DPP-IV, and ACE activity [101]. Soybean hydrolysates with a molecular weight of < 3 kDa prepared by chymotrypsin digestion exhibited reduced liposome oxidation and also showed the highest free FRSA [126]. In addition to molecular weight, the antioxidant activity and FRSA of soy hydrolysates corresponded with their amino acid composition, in particular, increased content of Phe and decreased content of Lys (Table 1) [127].…”

Section: Antioxidative Propertiesmentioning

confidence: 99%

Beneficial Effects of Soybean-Derived Bioactive Peptides

Kim

Yang²,

Kim

2021

IJMS

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Peptides present in foods are involved in nutritional functions by supplying amino acids; sensory functions related to taste or solubility, emulsification, etc.; and bioregulatory functions in various physiological activities. In particular, peptides have a wide range of physiological functions, including as anticancer agents and in lowering blood pressure and serum cholesterol levels, enhancing immunity, and promoting calcium absorption. Soy protein can be partially hydrolyzed enzymatically to physiologically active soy (or soybean) peptides (SPs), which not only exert physiological functions but also help amino acid absorption in the body and reduce bitterness by hydrolyzing hydrophobic amino acids from the C- or N-terminus of soy proteins. They also possess significant gel-forming, emulsifying, and foaming abilities. SPs are expected to be able to prevent and treat atherosclerosis by inhibiting the reabsorption of bile acids in the digestive system, thereby reducing blood cholesterol, low-density lipoprotein, and fat levels. In addition, soy contains blood pressure-lowering peptides that inhibit angiotensin-I converting enzyme activity and antithrombotic peptides that inhibit platelet aggregation, as well as anticancer, antioxidative, antimicrobial, immunoregulatory, opiate-like, hypocholesterolemic, and antihypertensive activities. In animal models, neuroprotective and cognitive capacity as well as cardiovascular activity have been reported. SPs also inhibit chronic kidney disease and tumor cell growth by regulating the expression of genes associated with apoptosis, inflammation, cell cycle arrest, invasion, and metastasis. Recently, various functions of soybeans, including their physiologically active functions, have been applied to health-oriented foods, functional foods, pharmaceuticals, and cosmetics. This review introduces some current results on the role of bioactive peptides found in soybeans related to health functions.

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“…Epidemiologists recommend the intake of more legumes (chickpea, soybean, lentil, cowpea etc.) and fruits because they are rich in natural antioxidants, moreover, it may also help in alleviating any potential nutritional gap in the body caused by unhealthy and low quality food consumption (Diniyah et al, 2020;Swallah et al, 2020;Matemu et al, 2021). Currently, several in vitro methods are being used to determine the different antioxidants found in plants (Alam et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Biochemical Profiling for Antioxidant and Therapeutic Potential of Pakistani Chickpea (Cicer arietinum L.) Genetic Resource

2021

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In Pakistan, chickpeas (Cicer arietinum L.) are the largest grown legume crops, especially in desert areas. Along with an excellent source of nutrition, chickpea seeds have discernible medicinal and antioxidant characteristics. The diverse set of 90 chickpea genotypes (66 desi and 24 kabuli) were collected from different research zones in Pakistan, and seed flour was used for biochemical profiling. Genotypes were significantly different (Tukey HSD test, P < 0.05) for the traits under investigation. In non-enzymatic antioxidants, highest seed total phenolic contents (TPC) (34725 ± 275 μM/g s. wt.) was found in CM-98 (desi), ascorbic acid (AsA) (69.23 ± 2.25 μg/g s. wt.) in WH-3 (desi), and total flavonoid content (TFC) (394.98 ± 13.06 μg/mL sample) was detected in WH-11 (desi). In the class of enzymatic antioxidants, the highest seed ascorbate peroxidase (APX) (1680 ± 40 Units/g s. wt.) was detected in Tamman-2013 (kabuli), peroxidases (POD) (2564.10 ± 233.10 Units/g s. wt.) activity in CM1235/08 (desi), and superoxide dismutase (SOD) (279.76 ± 50 Units/g s. wt.) was detected in CH24/11 (desi). Highest seed catalase activity (CAT) (893 ± 50 Units/g s. wt.) and proline content (272.50 ± 20.82 μg/g s. wt.) was detected in an ICC-4951 (desi). In hydrolytic enzymes, the highest activity of esterase (37.05 μM/min/g s. wt) was found in, CH56/09(Kabuli), protease (11080 ± 10 Units/g s. wt.) in Karak-2 (desi), and α-amylase (213.02 ± 3.20 mg/g s. wt.) was observed in CH74/08 (kabuli). In other biochemical parameters, the highest seed total oxidant status (TOS) (356 ± 17.50 μM/g s. wt.) was detected in CM3457/91 (desi); malondialdehyde (MDA) content (295.74 ± 3.097 uM/g s. wt.) was observed in CM-2008 (kabuli), and total antioxidant capacity (TAC) (8.36 ± 0.082 μM/g s. wt.) was found in CM-72 (desi). In case of pigment analysis, Sheenghar-2000 (desi) depicted highest lycopene (12.579 ± 0.313 μg/g s. wt.) and total carotenoids (58.430.23 ± 0.569 μg/g s. wt.) contents. For seed therapeutic potential, the highest seed α-amylase inhibition (82.33 ± 8.06%) was observed in CM-88 (desi), while WH-1, WH-6, and ICCV-96030 (desi) depicted the highest value for seed anti-inflammatory potential (78.88 ± 0.55%). Genotypes with the highest antioxidant and therapeutic potential can be utilized as a natural antioxidant source and in breeding programs aimed at improving these traits in new breeding lines.

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Health Benefits of Antioxidative Peptides Derived from Legume Proteins with a High Amino Acid Score

Cited by 67 publications

References 121 publications

Production and Characterization of Medium-Sized and Short Antioxidant Peptides from Soy Flour-Simulated Gastrointestinal Hydrolysate

Production and Characterization of Medium-Sized and Short Antioxidant Peptides from Soy Flour-Simulated Gastrointestinal Hydrolysate

Beneficial Effects of Soybean-Derived Bioactive Peptides

Biochemical Profiling for Antioxidant and Therapeutic Potential of Pakistani Chickpea (Cicer arietinum L.) Genetic Resource

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