Hearing Loss with Semicircular Canal Transection and <i>Pseudomonas Aeruginosa</i> Otitis Media

Prevatt, Angela R.; Sedwick, Jack D.; Gajewski, Byron; Antonelli, Patrick J.

doi:10.1016/j.otohns.2004.01.017

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…Prevatt et al (2004) demonstrated that P. aeruginosa in the middle ear may increase the risk of sensorineural hearing loss. Using an animal model in guinea pigs, the authors concluded that rupture of the tympanic membrane with the presence of P. aeruginosa in the middle ear was associated with a significant risk of sensorineural hearing loss in the animals.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals with Cleft Palate: Bacteriological Culture and Polymerase Chain Reaction

Weckwerth

Franco

Lopes

et al. 2014

The Cleft Palate Craniofacial Journal

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Section: Discussionmentioning

confidence: 99%

Bacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals with Cleft Palate: Bacteriological Culture and Polymerase Chain Reaction

Weckwerth

Franco

Lopes

et al. 2014

The Cleft Palate Craniofacial Journal

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“…Moreover, P . aeruginosa is associated with keratitis [ 11 ] and chronic suppurative otitis media [ 12 ] leading to visual impairment and deafness [ 13 , 14 ]. P .…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas aeruginosa MifS-MifR Two-Component System Is Specific for α-Ketoglutarate Utilization

et al. 2015

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Pseudomonas aeruginosa is a Gram-negative, metabolically versatile opportunistic pathogen that elaborates a multitude of virulence factors, and is extraordinarily resistant to a gamut of clinically significant antibiotics. This ability, in part, is mediated by two-component regulatory systems (TCS) that play a crucial role in modulating virulence mechanisms and metabolism. MifS (PA5512) and MifR (PA5511) form one such TCS implicated in biofilm formation. MifS is a sensor kinase whereas MifR belongs to the NtrC superfamily of transcriptional regulators that interact with RpoN (σ54). In this study we demonstrate that the mifS and mifR genes form a two-gene operon. The close proximity of mifSR operon to poxB (PA5514) encoding a ß-lactamase hinted at the role of MifSR TCS in regulating antibiotic resistance. To better understand this TCS, clean in-frame deletions were made in P. aeruginosa PAO1 creating PAO∆mifS, PAO∆mifR and PAO∆mifSR. The loss of mifSR had no effect on the antibiotic resistance profile. Phenotypic microarray (BioLOG) analyses of PAO∆mifS and PAO∆mifR revealed that these mutants were unable to utilize C5-dicarboxylate α-ketoglutarate (α-KG), a key tricarboxylic acid cycle intermediate. This finding was confirmed using growth analyses, and the defect can be rescued by mifR or mifSR expressed in trans. These mifSR mutants were able to utilize all the other TCA cycle intermediates (citrate, succinate, fumarate, oxaloacetate or malate) and sugars (glucose or sucrose) except α-KG as the sole carbon source. We confirmed that the mifSR mutants have functional dehydrogenase complex suggesting a possible defect in α-KG transport. The inability of the mutants to utilize α-KG was rescued by expressing PA5530, encoding C5-dicarboxylate transporter, under a regulatable promoter. In addition, we demonstrate that besides MifSR and PA5530, α-KG utilization requires functional RpoN. These data clearly suggests that P. aeruginosa MifSR TCS is involved in sensing α-KG and regulating its transport and subsequent metabolism.

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“…13 Additionally, the hearing loss appears to be primarily sensorineural with little or no conductive component. 14 Infection with Pseudomonas aeruginosa (PA) has been shown to be an important part of the development of hearing loss, 15 whereas hearing loss due to injury in the presence of other bacteria is more inconsistent. 16 …”

Section: Introductionmentioning

confidence: 99%

AM‐111 prevents hearing loss from semicircular canal injury in otitis media

2009

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Objectives/Hypothesis:Iatrogenic semicircular canal (SC) transection during mastoidectomy for chronic otitis media often leads to profound hearing loss. AM‐111, an apoptosis inhibitor, has been shown to mitigate hearing loss resulting from a variety of inner ear injuries. The goal of this study was to determine if round window application of AM‐111 following SC transection in the presence of Pseudomonas aeruginosa otitis media (PA‐OM) may reduce the associated hearing loss.Study Design:Prospective, randomized, controlled study in an animal model.Methods:PA‐OM was induced bilaterally in 34 guinea pigs. After 3 days, both bullae were opened and the lateral SC of one ear was transected. AM‐111 or vehicle was applied topically to the round window of the ear that had undergone SC transection. Hearing was assessed with auditory brainstem responses.Results:The mean change in hearing thresholds was significantly less in transected ears treated with AM‐111 than those receiving vehicle alone when testing with clicks (22.1 dB vs. 35.0 dB; P = .019) and at 4kHz (11.3 dB vs. 40.0 dB; P = .021). A similar trend was shown with 16 kHz tone pips (27.7 dB vs. 41.1 dB; P = .119).Conclusions:AM‐111 prevents hearing loss from SC transection in the guinea pig model of PA‐OM. Laryngoscope, 2010

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Hearing Loss with Semicircular Canal Transection and Pseudomonas Aeruginosa Otitis Media

Abstract: Factors unique to PA infection may be responsible for SNHL with labyrinthine injury in humans with chronic otitis media.

Cited by 10 publications

References 18 publications

Bacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals with Cleft Palate: Bacteriological Culture and Polymerase Chain Reaction

Bacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals with Cleft Palate: Bacteriological Culture and Polymerase Chain Reaction

Pseudomonas aeruginosa MifS-MifR Two-Component System Is Specific for α-Ketoglutarate Utilization

AM‐111 prevents hearing loss from semicircular canal injury in otitis media

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