Heat induced aggregation of gold nanorods for rapid visual detection of lysozyme

Moghadam, Tahereh Tohidi; Ranjbar, Bijan

doi:10.1016/j.talanta.2015.06.025

Cited by 21 publications

(13 citation statements)

References 43 publications

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“…GNRs were synthesized using the previously described seed-mediated growth method reported by Tohidi Moghadam and Nikoobakht [31, 32]. Briefly, the seed solution was prepared by mixing CTAB (7.5 mL, 0.095 M) and HAuCl 4 ·3H 2 O (250 μL, 0.01 M) followed by mixing with fresh ice-cold NaBH 4 (600 μL, 0.01 M) and rapid inversion for 2 min to form a yellow–brown solution.…”

Section: Methodsmentioning

confidence: 99%

A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli

2019

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BackgroundCampylobacteriosis is a zoonotic infectious disease that can be mostly undiagnosed or unreported due to fastidious Campylobacter species. The aim of this study was to develop a simple, sensitive, and quick assay for the detection of Campylobacter spp. and taking advantage of the great sensitivity of gold nanorods (GNRs) to trace changes in the local environment and interparticle distance.MethodsCharacterized GNRs were modified by specific ssDNA probes of cadF gene. First, the biosensor was evaluated using recombinant plasmid (pTG19-T/cadF) and synthetic single-stranded 95 bp gene, followed by a collection of the extracted DNAs of the stool samples. The sensing strategy was compared by culture, PCR, and real-time PCR.Results and discussionAnalysis of 283 specimens showed successful detection of Campylobacter spp. in 44 cases (16%), which was comparable to culture (7%), PCR (15%), and real-time PCR (18%). In comparison with real-time PCR, the sensitivity of the biosensor was reported 88%, while the specificity test for all assays was the same (100%). However, it was not able to detect Campylobacter in 6 positive clinical samples, as compared to real-time PCR. The limit of detection was calculated to be the same for the biosensor and real-time PCR (102 copy number/mL).ConclusionsTaking high speed and simplicity of this assay into consideration, the plasmonic nanobiosensor could pave the way in designing a new generation of diagnostic kits for detection of C. jejuni and C. coli species in clinical laboratories.Electronic supplementary materialThe online version of this article (10.1186/s12951-019-0476-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli

2019

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“…Engineering cardiac patches for treating damaged heart tissues after a heart attack or developing neural networks of higher biocompatibility and signal transfer efficiency in neuroscience and neural engineering can be highlighted as the promising biomedical applications of such hybrid nanoscaffolds. Moreover, taking the advantage of outstanding surface plasmon resonance properties of GNRs and their extreme sensitivity to trace changes in their local environment, a hybrid system consisting of such tiny rod-shaped nanostructures and amyloid fibrils might pave the way for developing a new biosensing strategy for the upcoming generation of plasmonic nanobiosensors and early diagnosis of pathogens and cancers 30 .…”

Section: Resultsmentioning

confidence: 99%

Assembly of Gold Nanorods on HSA Amyloid Fibrils to Develop a Conductive Nanoscaffold for Potential Biomedical and Biosensing Applications

Taheri

Akhtari

Moghadam

et al. 2018

Sci Rep

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Today, Gold Nanorods have promised variety of applications in conjugation with biomolecules of interest. Discovery of functional amyloids has also been highlighted with possible use in designing high performance materials. To exploit dual properties of both Nano and Bio counterparts in new functional materials, this effort has focused on synthesis of a potential hybrid system of Gold nanorods (GNRs) and HSA amyloid fibrils to develop a conductive nanoscaffold. UV-Vis spectroscopy, Thioflavin T (ThT) assay, Far-UV Circular Dichroism (CD) spectropolarimetry, fluorescence and Transmission Electron microscopy were used to characterize formation of the nanostructures and amyloid fibrils. Surface plasmon resonance of GNRs was also monitored upon interaction with HSA amyloid fibrils, showing that the plasmonic component of the hybrid system has maintained its characteristic rod morphology without any perturbations. Analysis of Nyquist plots for the hybrid nanoscaffold showed that the electronic behavior of the hybrid system has been enhanced due to the presence of the assembled GNRs. Results of this investigation highlight the possibility of fabricating hybrid nano-bioscaffolds as promising candidates in versatile biomedical and biosensing applications.

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“…Sequential seed mediated growth methods were used for gold nanorods 45 . In brief, small spherical gold nanoparticles (seeds) were synthesized by mixing aqueous solutions of CTAB (7.5 mL, 0.095 M), HAuCl 4 (250 μL, 0.01 M) and ice-cold NaBH4 solution (600 μL, 0.01 M), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Formation of rod-shaped nanostructures was initiated, accompanied by change in the solution color. It is worth noting that termination of the reaction might take several hours 45 . The average size of GNRs and their distribution was determined by transmission electron microscopy (TEM).…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of a Nano-Polyplex system of GNRs-PDMAEA-pDNA: An Inert Self-Catalyzed Degradable Carrier for Facile Gene Delivery

Dinari

Moghadam

Abdollahi

et al. 2018

Sci Rep

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Engineering molecules at nano-scale is a promising approach in targeting and curing diseases. In this research, fabricated new hybrid system called nano-polyplex represents an example of the molecular engineering at nano-scale. Polymer of PDMAEAs with four different molecular weights were synthesized using the RAFT method, attached onto the gold nano-rod surface, which modified and produced a safe novel system with an average size less than 100 nm. The hybrid system was characterized by ultra violet-visible spectrophotometer (UV-Vis), dynamic light scattering (DLS), 1H NMR, gel permeation chromatography (GPC), Fourier transform-infrared (FT-IR) spectroscopy, Zeta potential analyzer and transmission electron microscopy (TEM). Features of higher transfection and lower toxicity compared to the previously reported polyplex of PDMAEA, as well as the gold standard PEI, have been shown in all molecular weights and defined N/P ratios (10–200). The ideal physicochemical properties for escaping from the cell barriers, covering the large volume of genetic material (pDNA) and high efficiency of loading polyplexes on GNRs’ surface make it an ideal carrier. The results of this effort pave way in designing a new generation of nanoparticle-based delivery systems for nucleic acid therapy and gene editing.

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Heat induced aggregation of gold nanorods for rapid visual detection of lysozyme

Cited by 21 publications

References 43 publications

A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli

A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli

Assembly of Gold Nanorods on HSA Amyloid Fibrils to Develop a Conductive Nanoscaffold for Potential Biomedical and Biosensing Applications

Synthesis and Characterization of a Nano-Polyplex system of GNRs-PDMAEA-pDNA: An Inert Self-Catalyzed Degradable Carrier for Facile Gene Delivery

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