Synthesis and Characterization of a Nano-Polyplex system of GNRs-PDMAEA-pDNA: An Inert Self-Catalyzed Degradable Carrier for Facile Gene Delivery

Abstract

Engineering molecules at nano-scale is a promising approach in targeting and curing diseases. In this research, fabricated new hybrid system called nano-polyplex represents an example of the molecular engineering at nano-scale. Polymer of PDMAEAs with four different molecular weights were synthesized using the RAFT method, attached onto the gold nano-rod surface, which modified and produced a safe novel system with an average size less than 100 nm. The hybrid system was characterized by ultra violet-visible spectrophotometer (UV-Vis), dynamic light scattering (DLS), 1H NMR, gel permeation chromatography (GPC), Fourier transform-infrared (FT-IR) spectroscopy, Zeta potential analyzer and transmission electron microscopy (TEM). Features of higher transfection and lower toxicity compared to the previously reported polyplex of PDMAEA, as well as the gold standard PEI, have been shown in all molecular weights and defined N/P ratios (10–200). The ideal physicochemical properties for escaping from the cell barriers, covering the large volume of genetic material (pDNA) and high efficiency of loading polyplexes on GNRs’ surface make it an ideal carrier. The results of this effort pave way in designing a new generation of nanoparticle-based delivery systems for nucleic acid therapy and gene editing.

Synergistic Antibacterial Activity and Wound Healing Properties of Selenium-Chitosan-Mupirocin Nanohybrid System: An in Vivo Study on Rat Diabetic Staphylococcus aureus Wound Infection Model

“…The number of 1×10 4 cells were seeded to the flask and incubated at 37 °C in a humidified atmosphere of 5% CO 2 up to 7 days. After the third passage, the cells were harvested by Trypsin-EDTA 0.25 (Aria CELL; Sigma Source, Iran) 30,31 . Toxicity assay of M, SeNPs, CCH, along with their dual and triple combinations were tested in four folds higher than MIC concentrations using MTT cell viability assay kit (DNA Biotech Co, Iran) on mouse fibroblast connective tissue cell lines (L929, Iran Biological Resource Center), according to the manufacturer's protocol.…”

Polymer nano-systems for the encapsulation and delivery of active biomacromolecular therapeutic agents

“…97 Other studies using PDMAEA or chitosan for the complexation of DNA showed similar results, suggesting that increasing the DNA/polymer ratio leads to the formation of durable complexes and provides a better coverage as well as a better release of DNA. 98,99 Another key parameter in the formation of the polyplexes is the molecular mass of the polymer employed. Studies have investigated the influence of the molecular mass of polyethylenimine (PEI) on its complexation performance.…”

Improved Nucleic Acid Therapy with Advanced Nanoscale Biotechnology

“…For example, a hybrid nanocarrier composed of Au NPs and poly(dimethylaminoethyl acrylate) increased the DNA therapeutic efficiency as well as reduced adverse effects. 133 Another hybrid nanogel composed of Au NPs, tumor-targeting endosomolytic peptides, and siRNA achieved a triple combination of chemotherapy, phototherapy, and RNAi therapy. 134 Table 2 includes some typical types of nanocarriers used for NA therapy and their clinical stages.…”