Heat-killed Lactobacillus Reuteri GMNL-263 Prevents Epididymal Fat Accumulation and Cardiac Injury in High-Calorie Diet-Fed Rats

Abstract

High-calorie diet-induced obesity leads to cardiomyocyte dysfunction and apoptosis. Impaired regulation of epididymal fat content in obese patients has been known to increase the risk of cardiac injury. In our study, a lactic acid bacteria, Lactobacillus reuteri GMNL-263, was evaluated for its potential to reduce body weight and body fat ratio and to prevent heart injury in rats with high-fat diet-induced obesity. Lactic acid bacteria supplementation restored the cardiac function and decreased the physiological changes in the heart of the obese rats. In addition, the Fas/Fas-associated protein pathway-induced caspase 3/e Poly polymerase mediated apoptosis in the cardiomyocytes of the obese rats was reversed in the Lr263-treated rats. These results reveal that fed with Lr-263 reduces body fat ratio, inhibits caspase 3-mediated apoptosis and restores cardiac function in obese rats through recovery of ejection fraction and fractional shortening. Our results indicated that the administration of Lr263 lactic acid bacteria can significantly down-regulate body fat and prevent cardiomyocyte injury in obese rats.

Cellular apoptosis and cardiac dysfunction in STZ‐induced diabetic rats attenuated by anthocyanins via activation of IGFI‐R/PI3K/Akt survival signaling

“…Suppression of caspase activation and reduction of cytochrome c has been evidently correlated as a crucial event in the suppression of cardiac apoptosis associated with cardiac damages induced by DM, obesity and as well as drug overdose . Our present results also correlate with our previous reports on the cardio‐protective effects of other agents such as probiotics, co‐enzyme Q10, deep sea minerals, diallyl trisulfide and extracts of Alpinate oxyphyllae MIQ and Andrographis paniculata …”

Probiotic Lactobacillus spp. Act Against Helicobacter pylori-induced Inflammation

“…Multidrug resistant (MDR) H. pylori strains (v633 and v1354) were clinical isolates, which were characterized as resistant to both metronidazole and clarithromycin [32]. All H. pylori strains were routinely cultured on Brucella blood agar plates (Becton Dickinson, Franklin Lakes, NJ, USA) containing 10% sheep blood under 5% CO 2 and 10% O 2 conditions at 37°C for 48 h. Lactobacillus rhamnosus GMNL-74 (GM-020 ® , CCTCC M 203098, BCRC 910236) and Lactobacillus acidophilus GMNL-185 (CCTCC M 2017764, BCRC 910774) were cultured on Lactobacilli MRS agar plates (Becton Dickinson) at 37 °C for 24 h as described previously [33]. To activate Lactobacillus spp., the bacterial strains were cultured in MRS broth at 37 °C for 24 h and then subjected to each experimental study.…”

Role of Lactobacillus reuteri in Human Health and Diseases

“…Treatment with L. reuteri GMNL-263 reduced the body weight as well as the percentages of adipose tissue and liver to body weight. Interestingly, heat-killed GMNL-263 appeared to have a very similar beneficial function ( Hsieh et al, 2016 ; Liao et al, 2016 ). L. reuteri 6475 has also been shown to be beneficial against obesity in mice ( Poutahidis et al, 2013b ).…”