Heat production of brown adipocyte in FOK rat at high temperature in vitro

Tanaka, Eriko; Yamakawa, Akihiko; Furuyama, Fujiya; Nishino, Hoyoku; Yamamura, Masaichi; Nakano, S

doi:10.1016/0928-4680(94)90446-4

Cited by 3 publications

(6 citation statements)

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“…This would be advantageous to animals resistant to a hot environment. In a previous paper, we demonstrated that 10 9 M isoproterenol increased heat production in Wistar rats [17,18]. In the present study, an equivalent result was obtained in WKAH rats, but not in FOK rats, at both normal and high temperatures.…”

Section: Unpaired T-test)supporting

confidence: 69%

“…Since in heat-acclimated animals, it was found that certain suppressive modifications of the metabolic oxidative processes took place [2,4,13,21], it may be assumed that similar changes would occur in FOK rats without exposure to heat. The high concentration (10 6 M) of isoproterenol was thought to be the maximal stimulation [17,18], where the maximal heat production was similar between the two strains.…”

Section: Unpaired T-test)mentioning

confidence: 99%

“…After weighing the interscapular BAT, the brown adipocytes were isolated by the method of Bukowiecki et al [1] with some modifications [17]. Heat production by the cells was measured with and without isoproterenol either at 37.0 and 39.9°C using a microcalorimeter (Thermoactive Cell Analyzer ESCO-3000, ESCO Ltd., Tokyo) [17,18,20]. After all the cells, which were suspended in a total volume of 0.5 ml, were entered increase in WKAH and FOK rats at both temperatures (ANOVA), where heat production of FOK rats was not significantly different from that of WKAH rats (unpaired ttest).…”

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confidence: 99%

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Heat Production by Brown Adipocytes of FOK Rats Genetically Resistant to a Hot Environment.

Tanaka

Yamakawa

Ito

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. The FOK rat was established as an inbred strain with genotypic adaptation to a hot environment. To investigate the mechanism of the heat resistance of FOK rats, heat production by isolated brown adipocytes in FOK and WKAH rats was measured at 37.0 and 39.9°C. Basal heat production by brown adipocytes of FOK rats was not significantly different from that of WKAH rats. Isoproterenol increased the heat production in a dose-dependent manner in both strains, while 10 9 M isoproterenol caused a significant increase in WKAH rats and a negligible change in FOK rats. These results indicate that the reason why FOK rats are resistant to the severe heat is partly due to the low heat production by brown adipocytes. -KEY WORDS: body temperature, brown fat, calorimetry.

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Section: Unpaired T-test)supporting

confidence: 69%

Section: Unpaired T-test)mentioning

confidence: 99%

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confidence: 99%

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Heat Production by Brown Adipocytes of FOK Rats Genetically Resistant to a Hot Environment.

Tanaka

Yamakawa

Ito

et al. 1997

The Journal of Veterinary Medical Science

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“…It can be divided into two categories: the change caused by cell activities and the response to external stimuli . On the one hand, it is known that every cell activities, such as division, gene expression, enzyme reaction, metabolism and pathological state, is accompanied by temperature changes . Cancer cells have higher temperatures than those of normal tissues because of their enhanced metabolic activity, which has been proved by a clinical experiment .…”

Section: Introductionmentioning

confidence: 99%

“…Paulik et al used the infrared thermography to measure real‐time thermogenesis of isolated cells for the first time . The intracellular temperature changes unpredictably and non‐uniformly during different cellular activities, owing to the nonhomogeneity of cytoplasm . However, the spatial resolution of infrared thermography is only ≈10 um and the precision depends on the properties of the object studied .…”

Section: Introductionmentioning

confidence: 99%

Micro/Nanoscale Thermometry for Cellular Thermal Sensing

Bai

2016

Small

216

148

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Temperature is a key parameter to regulate cell function, and biochemical reactions inside a cell in turn affect the intracellular temperature. It's vitally necessary to measure cellular temperature to provide sufficient information to fully understand life science, while the conventional methods are incompetent. Over the last decade, many ingenious thermometers have been developed with the help of nanotechnology, and real-time intracellular temperature measurement at the micro/nanoscale has been realized with high temporal-spatial resolution. With the help of these techniques, several mechanisms of thermogenesis inside cells have been investigated, even in subcellular organelles. Here, current developments in cellular thermometers are highlighted, and a picture of their applications in cell biology is presented. In particular, temperature measurement principle, thermometer design and latest achievements are also introduced. Finally, the existing opportunities and challenges in this ongoing field are discussed.

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Red-Emitting Carbon Nanodot-Based Wide-Range Responsive Nanothermometer for Intracellular Temperature Sensing

Yang

Lin

et al. 2020

Anal. Chem.

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Precise sensing of intracellular temperature can provide plenty of information on disease-related cell states and promote the development of a diagnostic method. Fluorescence-based nanothermometers, as the “noncontact” sensors, exhibit great advantages over traditional thermometers due to the dual function of imaging and sensing at the molecular level. Herein, we report a red-emitting carbon nanodots (RCDs)-based nanothermometer for intracellular temperature sensing. Results indicate that RCDs exhibit favorable temperature-responsive fluorescence property with a good linear relationship, reversibility, and reproducibility under heating and cooling treatments in a wide range from 4 to 80 °C. Meanwhile, the RCDs possess satisfactory thermal sensitivity and temperature resolution, which are superior or comparable to the current nanothermometers. The low cytotoxicity and excellent temperature-responsive fluorescence property of RCDs have also been verified in living cell studies. Therefore, the RCDs will be a promising nanothermometer for intracellular temperature sensing in diverse areas.

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Heat production of brown adipocyte in FOK rat at high temperature in vitro

Cited by 3 publications

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Heat Production by Brown Adipocytes of FOK Rats Genetically Resistant to a Hot Environment.

Heat Production by Brown Adipocytes of FOK Rats Genetically Resistant to a Hot Environment.

Micro/Nanoscale Thermometry for Cellular Thermal Sensing

Red-Emitting Carbon Nanodot-Based Wide-Range Responsive Nanothermometer for Intracellular Temperature Sensing

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