Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish

Saju, Jolly M.; Hossain, Mohammad Sorowar; Liew, Woei Chang; Pradhan, Ajay; Thevasagayam, Natascha May; Tan, Lydia Shun En; Alladi, Anand; Olsson, Pia; Orbán, László

doi:10.1016/j.celrep.2018.11.090

Cited by 33 publications

(21 citation statements)

References 49 publications

(48 reference statements)

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“…HSF5 belongs to the heat shock transcription factor family, which is involved in differentiation, reproduction, and stress-induced adaptation [14]. Previous studies have revealed that HSF5 plays a critical role in germ cell development and meiotic progression [24,38]. However, the functional characterization of HSF5 involved in cancer and the immune response has not been conducted.…”

Section: Discussionmentioning

confidence: 99%

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Heat shock factor 5 correlated with immune infiltration serves as a prognostic biomarker in lung adenocarcinoma

Rusidanmu

Tang

et al. 2021

Int. J. Med. Sci.

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Lung adenocarcinoma (LUAD) is the predominant subtype of lung cancer with a relatively poor prognosis. The dramatic improvements of new immunotherapy strategies have shown promising results in lung cancer patients. This study aimed to elucidate the functions of immune-associated genes in LUAD prognosis and pathogenesis by analyzing public databases. We obtained expression profiles of LUAD patients from The Cancer Genome Atlas (TCGA) database and applied the ESTIMATE algorithm to calculate immune scores and stromal scores. A series of microenvironment-related genes with prognostic value was then identified. Of note, heat shock factor 5 (HSF5) was found to be decreased in LUAD patients and positively correlated with overall survival, which was further confirmed in the Gene Expression Omnibus (GEO) database. Moreover, Gene Ontology (GO) analysis based on the correlated genes of HSF5 demonstrated that HSF5 expression was significantly associated with the immune response and inflammatory activities. Based on the Tumor IMmune Estimation Resource (TIMER) and Gene Expression Profiling Interactive Analysis (GEPIA) datasets, HSF5 expression showed strong correlations with various immune cell infiltration and diverse immune marker sets. These findings suggest that HSF5 can be used as a promising biomarker for determining prognosis and immune infiltration in LUAD patients.

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Section: Discussionmentioning

confidence: 99%

“…However, HSF2 has appeared to decrease in a wide range of cancers and act as a tumor suppressor [22,23]. To date, only a few studies have focused on HSF5, and its detailed functional characterization in tumors has not been performed [24].…”

Section: Ivyspringmentioning

confidence: 99%

Heat shock factor 5 correlated with immune infiltration serves as a prognostic biomarker in lung adenocarcinoma

Rusidanmu

Tang

et al. 2021

Int. J. Med. Sci.

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“…The transcripts of ar peak at 16 dpf and 22 dpf, and are subsequently maintained at a high stable level across the presumptive male development stage, suggesting that it is essential for male differentiation and maintenance [35] , [39] . Saju et al found that heat shock transcription factor family member 5 ( hsf5 ), whose expression increased from 35dpf, plays an important role in early stage of spermatogenesis and fertility in males [40] .…”

Section: Gene Network For Reproductive Development In Zebrafishmentioning

confidence: 99%

“…ar -null male zebrafishes appear with female secondary sex characteristics and are sterile, having smaller testes and blocked spermatogenesis [41] . hsf5 −/− mutant males are infertile with decreased sperm count, abnormal sperm morphology and decreased sperm motility, however, females are fertile [40] .…”

Section: Gene Network For Reproductive Development In Zebrafishmentioning

confidence: 99%

Zebrafish as an emerging model to study gonad development

Chen

2020

Computational and Structural Biotechnology Journal

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The zebrafish ( Danio rerio ) has emerged as a popular model organism in developmental biology and pharmacogenetics due to its attribute of pathway conservation. Coupled with the availability of robust genetic and transgenic tools, transparent embryos and rapid larval development, studies of zebrafish allow detailed cellular analysis of many dynamic processes. In recent decades, the cellular and molecular mechanisms involved in the process of gonad development have been the subject of intense research using zebrafish models. In this mini-review, we give a brief overview of these studies, and highlight the essential genes involved in sex determination and gonad development in zebrafish.

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“…Under stress condition in poultries all heat shock factors are activated. In animals, four HSFs was known till 2018, when Saju and his collages found [31] the fth HSF, HSF5 in Danio rerio. This HSF5 is essential for the spermatogenesis in zebra sh.…”

Section: Introductionmentioning

confidence: 99%

Examination the effect of post-hatch heat-treatment and heat-stress in Transylvanian Naked Neck chicken

Tóth

Szabadi

Lázár

et al. 2020

Preprint

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Background One of the most critical global problem nowadays is the increased environmental temperature. Agriculture is very susceptible to this adverse effect because the productivity of animals and poultry decreased. Although several studies reported the effects of heat-stress in chicken, the expression profile of heat-shock proteins and heat shock factors was not investigated in the gonads and germ cells of Transylvanian Naked Neck chickens. Methods In the first experiment, 24 hours after hatching 80 chicks were heat treated on 38.5oC ambient temperature with 60% humidity for 12 hours. After maturation, their primary productivity parameters, such as egg production, abnormalities in embryo development, sperm quantity, concentration, and motility were studied following two weeks of heat-stress on 30 °C room temperature. In the second experiment, the thermal manipulation of 60 chicks was the same but 15 treated and 15 control chicks were sacrificed immediately after the treatment. The other 15–15 chickens were raised to maturity. Expression levels for two heat-shock proteins and four heat shock factors were determined by real-time PCR in the gonads of heat-treated and heat-stressed chickens. Results We found that the heat-treated layers had significantly higher egg production than the control group in heat-stressed conditions. In cockerels, the sperm quality did not differ significantly between the heat-treated and heat-stressed group and the heat-stressed but not heat-treated group. We examined the expression pattern of HSPs and HSFs in the gonads. We found that the expression of HSP90 and HSF4 increased significantly (p < 0.05) in heat-treated female chick gonads but in adult females the expression of HSF2 and HSF3 were significantly lower compared to the control. In case of adult heat-treated males, the HSP70, HSF1 and HSF3 expression levels showed a significant increase in both gonads, compared to the control expression levels (P < 0.05). Conclusion Heat shock proteins and heat-shock factors protect cells against different stressors, including heat stress. Our findings show a significant effect on egg production but not on the sperm quality after post-hatch heat treatment in heat stress condition. The presented significant differences might be related to the increased expression level of HSP90 and HSF4 in heat-treated chickens.

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Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish

Cited by 33 publications

References 49 publications

Heat shock factor 5 correlated with immune infiltration serves as a prognostic biomarker in lung adenocarcinoma

Heat shock factor 5 correlated with immune infiltration serves as a prognostic biomarker in lung adenocarcinoma

Zebrafish as an emerging model to study gonad development

Examination the effect of post-hatch heat-treatment and heat-stress in Transylvanian Naked Neck chicken

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