Heat Shock Protein 70 Expression in Juvenile Eastern Oysters,<i>Crassostrea virginica</i>(Gmelin, 1791), Exposed to Anoxic Conditions

Ueda, Nobuo; Ford, Courtney; Rikard, Scott; Wallace, Richard K.; Boettcher, Anne

doi:10.2983/035.028.0414

Cited by 11 publications

(5 citation statements)

References 33 publications

(36 reference statements)

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“…Results from this study indicate that nylon MF approximately 500 μm in length at low concentrations do not block the digestive system nor cause tissue necrosis or excessive stress responses (Table S2). Mussels from all treatment groups showed a low level of DGA, a response that can be indicative of stressors of many kinds (Winstead, 1995). DGA analysis in bivalves has been used in conjunction with other biomarkers to evaluate oil‐spill effects and recvery, and has even been linked to climate variation (Garmendia et al, 2011; Kim & Powell, 2009).…”

Section: Discussionmentioning

confidence: 99%

Nylon microfibers develop a distinct plastisphere but have no apparent effects on the gut microbiome or gut tissue status in the blue mussel, Mytilus edulis

Collins,

Griffin,

Holohan

et al. 2023

Environmental Microbiology

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Ingestion of microplastics (MP) by suspension‐feeding bivalves has been well‐documented. However, it is unclear whether exposure to MP could damage the stomach and digestive gland (gut) of these animals, causing ramifications for organism and ecosystem health. Here, we show no apparent effects of nylon microfiber (MF) ingestion on the gut microbiome or digestive tissues of the blue mussel, Mytilus edulis. We exposed mussels to two low concentrations (50 and 100 particles/L) of either nylon MF or Spartina spp. particles (dried, ground marsh grass), ca. 250–500 μm in length, or a no particle control laboratory treatment for 21 days. Results showed that nylon MF, when aged in coarsely filtered seawater, developed a different microbial community than Spartina spp. particles and seawater, however, even after exposure to this different community, mussel gut microbial communities resisted disturbance from nylon MF. The microbial communities of experimental mussels clustered together in ordination and were similar in taxonomic composition and measures of alpha diversity. Additionally, there was no evidence of damage to gut tissues after ingestion of nylon MF or Spartina spp. Post‐ingestive particle processing likely mediated a short gut retention time of these relatively large particles, contributing to the negligible treatment effects.

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Section: Discussionmentioning

confidence: 99%

Nylon microfibers develop a distinct plastisphere but have no apparent effects on the gut microbiome or gut tissue status in the blue mussel, Mytilus edulis

Collins,

Griffin,

Holohan

et al. 2023

Environmental Microbiology

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“…HSP70 is one of the most important family of 70‐kDa chaperones which functions in mitigating cellular apoptosis and oxidative stress during heat stress (Li et al, ; Padmini & Tharani, ). Rising water temperatures have resulted in high expression of HSP70 in a wide variety of fish (Sørensen, Kristensen, & Loeschke, ) and shellfish species, including oysters (Bonaventura et al, ; Farcy, Voiseux, Lebel, & Fievet, ; Ueda et al, ). Previous studies have shown that short‐term exposure to higher temperatures (28 and 32°C for 1 week) not only increases HSP70 expression in testicular tissues (e.g., Atlantic sea urchin; Johnstone et al, ), but also increases its expression in whole embryos of purple sea urchin (Roccheri, Di Bernardo, & Giudice, ), embryonic cells of chinook salmon (Misra, Zafarullah, Price‐Haughey, & Gedamu, ) and hepatocytes of rainbow trout (Boone & Vijayan, ).…”

Section: Discussionmentioning

confidence: 99%

“…Slides were then incubated with mouse monoclonal anti‐HSP70 (Millipore Sigma), mouse monoclonal anti‐NTP (Santa Cruz Biotechnology, Dallas, TX) and rabbit polyclonal anti‐DNP (Thermo Fisher Scientific) primary antibodies (antibody dilution 1:100 with 1X PBS) at 4°C overnight. The HSP70, NTP, and DNP antibodies were validated previously in oysters and sea urchin gonad tissues (Bonaventura et al, ; Johnstone et al, ; Ueda & Boettcher, ; Ueda et al, ). The negative control slides were incubated with only 1X PBS instead of HSP70, NTP, or DNP primary antibodies at 4 o C overnight.…”

Section: Methodsmentioning

confidence: 99%

“…HSPs not only serve as regulator of internal functions, but are also chaperone proteins that assist in folding, assembling, and transporting of other essential proteins (Ueda & Boettcher, ). Changes in HSPs’ (e.g., HSP70, HSP72, HSP77) expression have also been linked to developmental changes during embryogenesis, as well as early spat development in oysters (Ueda & Boettcher, ; Ueda, Ford, Rikard, Wallace, & Boettcher, ). During thermal stress, heat shock protein‐70 (HSP70) transcription rates increase rapidly and cause a series of posttranscriptional events to occur, including a block on pre‐mRNA processing and affects messenger RNA (mRNA) stability (Theodorakis & Morimoto, ).…”

Section: Introductionmentioning

confidence: 99%

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Short‐term heat stress impairs testicular functions in the American oyster, Crassostrea virginica: Molecular mechanisms and induction of oxidative stress and apoptosis in spermatogenic cells

Nash

Rahman

2019

Molecular Reproduction Devel

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Global temperature is increasing due to anthropogenic activities. Abnormal temperature has devastating effects on growth, reproduction, and development of aquatic organisms. In this study, we examined the effects of short-term exposure to elevated temperatures (28 and 32°C for 1 week) on testicular functions, heat shock protein-70 (HSP70), dinitrophenyl protein (DNP, a biomarker of reactive oxygen species [ROS]), and nitrotyrosine protein (NTP, an indicator of reactive nitrogen species [RNS]) expressions, protein carbonyl (PC, a measure of ROS) contents, nitrates/nitrites (NOx, a metabolite of nitric oxide) levels, extrapallial fluid (EPF)conditions, and cellular apoptosis in American oyster (Crassostrea virginica). Higher temperatures significantly decreased (~26%) sperm production in oysters compared with controls (24°C). HSP70, NTP, and DNP expressions were increased after heat exposure, consistent with increased EPF pH, and cellular apoptosis. The enhanced apoptosis in spermatogenic cells is associated with increased caspase 3/7 activity, PC contents, and NOx levels in testicular tissues. Together these results suggest that elevated temperature drastically increases oxidative stress and cellular apoptosis which in turn leads to decreased testicular functions in oysters. To the best of our knowledge, this study reports the first findings on the impacts of elevated temperatures on testicular functions in oysters. K E Y W O R D SAmerican oyster, apoptosis, heat shock protein, heat stress, oxidative stress, testis

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“…The role of different isoforms in invertebrates has not been examined. To date there have been mixed results reported for the usefulness of biomarkers for hypoxia in invertebrates [36] , [37] , indicating that more detailed study is needed and suggesting that multiple biomarkers might be important.…”

Section: Introductionmentioning

confidence: 99%

Biomarkers of Dissolved Oxygen Stress in Oysters: A Tool for Restoration and Management Efforts

2014

Self Cite

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The frequency and intensity of anoxic and hypoxic events are increasing worldwide, creating stress on the organisms that inhabit affected waters. To understand the effects of low dissolved oxygen stress on oysters, hatchery-reared oysters were placed in cages and deployed along with continuously recording environmental data sondes at a reef site in Mobile Bay, AL that typically experiences low oxygen conditions. To detect and measure sublethal stress, we measured growth and survival of oysters as well as expression of three biomarkers, heat shock protein 70 (HSP70), hypoxia inducible factor (HIF) and phospho-p38 MAP kinase, in tissues from juvenile and adult oysters. Survival rates were high for both juvenile and adult oysters. Expression levels of each of the 3 isoforms of HSP 70 were negatively correlated to dissolved oxygen (DO) concentrations, suggesting that HSP 70 is useful to quantify sublethal effects of DO stress. Results for HIF and phospho-p38 MAP kinase were inconclusive. Test deployments of oysters to assess expression of HSP 70 relative to environmental conditions will be useful, in addition to measuring abiotic factors, to identify appropriate sites for restoration, particularly to capture negative effects of habitat quality on biota before lethal impacts are incurred.

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Heat Shock Protein 70 Expression in Juvenile Eastern Oysters,Crassostrea virginica(Gmelin, 1791), Exposed to Anoxic Conditions

Cited by 11 publications

References 33 publications

Nylon microfibers develop a distinct plastisphere but have no apparent effects on the gut microbiome or gut tissue status in the blue mussel, Mytilus edulis

Nylon microfibers develop a distinct plastisphere but have no apparent effects on the gut microbiome or gut tissue status in the blue mussel, Mytilus edulis

Short‐term heat stress impairs testicular functions in the American oyster, Crassostrea virginica: Molecular mechanisms and induction of oxidative stress and apoptosis in spermatogenic cells

Biomarkers of Dissolved Oxygen Stress in Oysters: A Tool for Restoration and Management Efforts

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