Heat Shock Proteins and Blood-Feeding in Arthropods

Pereira, Marcos H.; Paim, Rafaela M.M.; Lahondère, Chloé; Lazzari, Cláudio R.

doi:10.1007/978-3-319-73377-7_13

Cited by 11 publications

(12 citation statements)

References 29 publications

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“…HSP expression is induced by a variety of stresses in insects, including heat and insecticide treatment [26,60]. We found that 12 DEGs are HSPs, all of them over-expressed in mosquito larvae treated with EEO.…”

Section: Heat Shock Proteinsmentioning

confidence: 82%

Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

et al. 2021

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Background Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. Methods and findings Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. Conclusions and significance Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.

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Section: Heat Shock Proteinsmentioning

confidence: 82%

Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

et al. 2021

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“…Most of the upregulated transcripts involved in protein modification encoded heat shock proteins or molecular chaperones, including 7 of the 25 most abundant transcripts (S3 Table). The rapid ingestion of large amounts (relative to their body size) of warm blood by arthropods induces mechanical, thermal, osmotic, and oxidative stress in the gut [32]. To deal with these stresses, arthropods produce heat shock proteins to moderate their influence and regulate the reparation of any damage or dysfunction they induce [32].…”

Section: Transcriptional Profile Of the Flea Gut In Response To Bloodmentioning

confidence: 99%

“…The rapid ingestion of large amounts (relative to their body size) of warm blood by arthropods induces mechanical, thermal, osmotic, and oxidative stress in the gut [32]. To deal with these stresses, arthropods produce heat shock proteins to moderate their influence and regulate the reparation of any damage or dysfunction they induce [32]. These proteins are also involved in the proper folding of newly synthesized proteins.…”

Section: Transcriptional Profile Of the Flea Gut In Response To Bloodmentioning

confidence: 99%

Transcriptomic profiling of the digestive tract of the rat flea, Xenopsylla cheopis, following blood feeding and infection with Yersinia pestis

et al. 2020

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Yersinia pestis, the causative agent of plague, is a highly lethal pathogen transmitted by the bite of infected fleas. Once ingested by a flea, Y. pestis establish a replicative niche in the gut and produce a biofilm that promotes foregut colonization and transmission. The rat flea Xenopsylla cheopis is an important vector to several zoonotic bacterial pathogens including Y. pestis. Some fleas naturally clear themselves of infection; however, the physiological and immunological mechanisms by which this occurs are largely uncharacterized. To address this, RNA was extracted, sequenced, and distinct transcript profiles were assembled de novo from X. cheopis digestive tracts isolated from fleas that were either: 1) not fed for 5 days; 2) fed sterile blood; or 3) fed blood containing~5x10 8 CFU/ml Y. pestis KIM6+. Analysis and comparison of the transcript profiles resulted in identification of 23 annotated (and 11 unknown or uncharacterized) digestive tract transcripts that comprise the early transcriptional response of the rat flea gut to infection with Y. pestis. The data indicate that production of antimicrobial peptides regulated by the immune-deficiency pathway (IMD) is the primary flea immune response to infection with Y. pestis. The remaining infection-responsive transcripts, not obviously associated with the immune response, were involved in at least one of 3 physiological themes: 1) alterations to chemosensation and gut peristalsis; 2) modification of digestion and metabolism; and 3) production of chitin-binding proteins (peritrophins). Despite producing several peritrophin transcripts shortly after feeding, including a subset that were infection-responsive, no thick peritrophic membrane was detectable by histochemistry or electron microscopy of rat flea guts for the first 24 hours following blood-feeding. Here we discuss the physiological implications of rat flea infection-responsive transcripts, the function of X. cheopis peritrophins, and the mechanisms by which Y. pestis may be cleared from the flea gut.

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“…quinquefasciatus may also synthesize heat shock proteins (HSPs), which typically function as chaperone proteins to prevent damage to existing proteins during times of stress, to recover from ingestion of a hot blood meal. Because the production of HSPs in response to thermal stress is a highly conserved physiological response to heat stress throughout the animal kingdom (Pereira et al, 2017), and Cx. pipiens has been shown to use these proteins after ingesting a hot blood-meal (Benoit et al, 2011), we suggest that Cx.…”

Section: Discussionmentioning

confidence: 99%

Beat the heat: Culex quinquefasciatus regulates its body temperature during blood feeding

Reinhold

Shaw

Lahondère

2021

Journal of Thermal Biology

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Heat Shock Proteins and Blood-Feeding in Arthropods

Cited by 11 publications

References 29 publications

Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

Transcriptomic profiling of the digestive tract of the rat flea, Xenopsylla cheopis, following blood feeding and infection with Yersinia pestis

Beat the heat: Culex quinquefasciatus regulates its body temperature during blood feeding

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