Heat shock transcription factor 1 promotes the proliferation, migration and invasion of osteosarcoma cells

Zhou, Zhenhua; Li, Yan; Jia, Qi; Wang, Zhiwei; Wang, Xudong; Hu, Jingjing; Xiao, Jianru

doi:10.1111/cpr.12346

Cited by 29 publications

(19 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other peculiarities in cancer cell behavior were also found to be suppressed by CL-43, more specifically, migration of HCT-116 cells (Figure 3A–3C ) and colony formation capacity (Figure 3D, 3E ). These effects clearly resemble the consequences of HSF1 knock-down, as shown recently [ 23 , 24 ]. We conclude that CL-43 by itself can reduce the growth of cancer cells and accordingly, their tumorigenicity.…”

Section: Discussionsupporting

confidence: 79%

Discovery and optimization of cardenolides inhibiting HSF1 activation in human colon HCT-116 cancer cells

et al. 2018

View full text Add to dashboard Cite

Combinational anticancer therapy demonstrates increased efficiency, as it targets different cell-survival mechanisms and allows the decrease of drug dosages that are often toxic to normal cells. Inhibitors of the heat shock response (HSR) are known to reduce the efficiency of proteostasis mechanisms in many cancerous cells, and therefore, may be employed as anti-tumor drug complements. However, the application of HSR inhibitors is limited by their cytotoxicity, and we suggested that milder inhibitors may be employed to sensitize cancer cells to a certain drug.We used a heat-shock element-luciferase reporter system and discovered a compound, CL-43, that inhibited the levels of heat shock proteins 40, 70 (Hsp70), and 90 kDa in HCT-116 cells and was not toxic for cells of several lines, including normal human fibroblasts. Consequently, CL-43 was found to reduce colony formation and motility of HCT-116 in the appropriate assays suggesting its possible application in the exploration of biology of metastasizing tumors. Importantly, CL-43 elevated the growth-inhibitory and cytotoxic activity of etoposide, cisplatin, and doxorubicin suggesting that the pro-drug has broad prospect for application in a variety of anti-tumor therapy schedules.

show abstract

Section: Discussionsupporting

confidence: 79%

Discovery and optimization of cardenolides inhibiting HSF1 activation in human colon HCT-116 cancer cells

et al. 2018

View full text Add to dashboard Cite

show abstract

“…All the 10 studies investigated the prognostic role of HSF1 in OS, and only three studies focused on disease-free survival (DFS). 9 , 15 , 16 HR with the corresponding 95% CI was directly extracted through multivariate analyses in seven studies, 8 – 14 and HR in the remaining three studies 15 – 17 was calculated from Kaplan–Meier survival curves by the Tierney’s methods. The quality of all the 10 studies was assessed by NOS, and the scores ranged from 5 to 8 (median 6.7), suggesting that the methodological quality was relatively high ( Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Identification of many prognostic factors in recent years has helped more accurate prognostic prediction of tumor patients. Growing studies have demonstrated that HSF1 is overexpressed in a series of solid tumors such as esophageal squamous cell carcinoma (ESCC), 8 , 9 breast cancer (BC), 10 , 11 hepatocellular carcinoma (HCC), 12 – 14 osteosarcoma, 15 non-small-cell lung cancer (NSCLC), 16 and pancreatic cancer. 17 Furthermore, evidence implies that the elevation of HSF1 expression is correlated with poor survival of tumor patients.…”

Section: Introductionmentioning

confidence: 99%

Prognostic role of HSF1 overexpression in solid tumors: a pooled analysis of 3,159 patients

Wan¹,

Liu²,

Luo³

et al. 2018

OTT

View full text Add to dashboard Cite

Background and objectiveHSF1 is reported to be overexpressed in various solid tumors and play a pivotal role in cancer progression. A meta-analysis was conducted to assess the potential prognostic role of HSF1 in patients with solid tumors.MethodsAn extensive electronic search of three databases was performed for relevant articles. The pooled hazard ratios (HRs) or odds ratios with their corresponding 95% CI were calculated with a random-effects model. Heterogeneity and publication bias analyses were also conducted.ResultsA total of 3,159 patients from 10 eligible studies were included into the analysis. The results showed that positive HSF1 expression was significantly correlated with poor overall survival in all tumors (HR=2.09; 95% CI: 1.62–2.70; P<0.001). Subgroup analysis revealed that there was a significant association between HSF1 overexpression and poor prognosis in esophageal squamous cell carcinoma (ESCC) (HR=1.83; 95% CI: 1.21–2.77; P=0.004), breast cancer (BC) (HR=1.52; 95% CI: 1.24–2.86; P<0.001), hepatocellular carcinoma (HR=3.02; 95% CI: 1.77–5.18; P<0.001), non-small-cell lung cancer (HR=2.19; 95% CI: 1.20–3.99; P=0.01), and pancreatic cancer (HR=2.58; 95% CI: 1.11–6.03; P=0.03) but not in osteosarcoma (HR=1.58; 95% CI: 0.47–5.35; P=0.46). In addition, HSF1 overexpression was significantly associated with some phenotypes of tumor aggressiveness including TNM stage, histological grade, lymph node metastasis, and vascular invasion.ConclusionHSF1 overexpression may prove to be an unfavorable prognostic biomarker for solid tumor patients.

show abstract

“…In breast cancer, a high HSF1 level was associated with histological grade, larger tumour size, and nodal involvement [ 14 ]. High expression of HSF1 protein is significantly associated with aggressive disease and poor survival in CRC, ESCC, ccRCC and osteosarcoma [ 12 , 13 , 16 – 18 ]. As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Increased expression of heat shock factor 1 (HSF1) is associated with poor survival in gastric cancer patients

Dai

Zhang

et al. 2018

Diagn Pathol

View full text Add to dashboard Cite

BackgroundHeat shock factor 1 (HSF1) was initially identified as a transcription factor encoding heat shock proteins, which assist in refolding or degrading damaged proteins. Recent studies have reported that HSF1 can act as an oncogene that regulates tumour progression. The present study aimed to elucidate the clinicopathological significance and prognostic value of HSF1 expression in gastric cancer (GC).MethodsThe data from The Cancer Genome Atlas (TCGA) were used to analyse HSF1 expression in GC and normal tissues, while 8 pairs of freshly frozen tissue samples were used to investigate HSF1 expression at the mRNA and protein levels in GC tissues and adjacent normal tissues using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting assays. The correlations between HSF1 expression and clinicopathological parameters, including the survival rate, were investigated in 117 GC tissue samples by immunohistochemical analysis.ResultsThe results of bioinformatics analysis, qRT-PCR, and western blot showed that HSF1 expression was higher in GC tissues than in normal tissues. High HSF1 expression was found in 54.7% (64/117) patients. Patients with high HSF1 expression had larger tumour size (P = 0.001), advanced Bornmann classification (P = 0.002), advanced depth of invasion (P = 0.015), lymph node metastasis (P<0.001), distant metastasis (P = 0.011) and tumour-node-metastasis (P<0.001). Moreover, the Kaplan-Meier and Cox proportional hazards analyses indicated that high HSF1 expression was significantly associated with poor overall survival and recurrence-free survival in GC patients and that high HSF1 expression was an independent prognostic factor for the long-term survival in GC patients.ConclusionsTaken together, our results show that high HSF1 expression is significantly correlated with advanced tumour progression and poor prognosis. In addition, HSF1 expression can serve as a biomarker for the prognosis of patients with GC.

show abstract

Heat shock transcription factor 1 promotes the proliferation, migration and invasion of osteosarcoma cells

Abstract: These data highlight an important role of HSF1 in proliferation, migration and invasion of osteosarcoma cells. Moreover, the expression of HSF1 was associated with prognosis in osteosarcoma.

Cited by 29 publications

References 32 publications

Discovery and optimization of cardenolides inhibiting HSF1 activation in human colon HCT-116 cancer cells

Discovery and optimization of cardenolides inhibiting HSF1 activation in human colon HCT-116 cancer cells

Prognostic role of HSF1 overexpression in solid tumors: a pooled analysis of 3,159 patients

Increased expression of heat shock factor 1 (HSF1) is associated with poor survival in gastric cancer patients

Contact Info

Product

Resources

About