Heat Stress Alters the Effect of Eimeria maxima Infection on Ileal Amino Acids Digestibility and Transporters Expression in Meat-Type Chickens

Ghareeb, Ahmed F. A.; Schneiders, Gustavo H.; Foutz, James C.; Milfort, Marie C.; Fuller, Alberta L.; Yuan, Jianmin; Rekaya, Romdhane; Aggrey, Samuel E.

doi:10.3390/ani12121554

Cited by 3 publications

(2 citation statements)

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“…Ileum cells upregulated the metabolism and degradation of multiple amino acids, such as lysine, histidine, valine, leucine, and isoleucine. This may plausibly be a compensatory mechanism to maintain cellular functions and energy production under low nutrient availability created by HS [15,16,[31][32][33]. The results show the upregulation of multiple aldehyde dehydrogenase (ALDH) genes.…”

Section: Ileum Tissue Transcriptome Change In Response To Hsmentioning

confidence: 94%

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Host transcriptome response to heat stress and Eimeria maxima infection in meat-type chickens

Ghareeb,

Foutz,

Schneiders

et al. 2024

PLoS ONE

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Eimeria (E.) maxima parasite infects chickens’ midgut disrupting the jejunal and ileal mucosa causing high morbidity and mortality. Heat stress (HS) is a seasonal stressor that impacts biological functions leading to poor performance. This study elucidates how HS, E. maxima infection, and their combination affect the ileum transcriptome. Two-hundred and forty 2-week-old males Ross708 chickens were randomly allocated into four treatment groups: thermoneutral-control (TNc), thermoneutral-infected (TNi), heat-stress control (HSc), and heat stress-infected (HSi), with 6 replicates each of 10 birds. Infected groups received 200x103 sporulated E. maxima oocysts/bird, and heat-treated groups were raised at 35°C. At 6-day post-treatment, ileums of five randomly selected chickens per group were sampled, RNA was extracted and sequenced. A total of 413, 3377, 1908, and 2304 DEGs were identified when applying the comparisons: TNc vs HSc, TNc vs TNi, HSi vs HSc, and TNi vs HSi, respectively, at cutoff ≥1.2-fold change (FDR: q<0.05). HSc vs TNc showed upregulation of lipid metabolic pathways and degradation/metabolism of multiple amino acids; and downregulation of most immune-related and protein synthesis pathways. TNc vs TNi displayed upregulation of most of immune-associated pathways and eukaryotic mRNA maturation pathways; and downregulation of fatty acid metabolism and multiple amino acid metabolism pathways including tryptophan. Comparing HSi versus HSc and TNi revealed that combining the two stressors restored the expression of some cellular functions, e.g., oxidative phosphorylation and protein synthesis; and downregulate immune response pathways associated with E. maxima infection. During E. maxima infection under HS the calcium signaling pathway was downregulated, including genes responsible for increasing the cytoplasmic calcium concentration; and tryptophan metabolism was upregulated, including genes that contribute to catabolizing tryptophan through serotonin and indole pathways; which might result in reducing the cytoplasmic pool of nutrients and calcium available for the parasite to scavenge and consequently might affect the parasite’s reproductive ability.

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Section: Ileum Tissue Transcriptome Change In Response To Hsmentioning

confidence: 94%

“…Exposing E. maximainfected chickens to HS-suppressed E. maxima gametogony. Broiler chickens exposed to a combination of HS and E. maxima infection exhibited higher values for the apparent ileal digestibility and less enterocytic damage than the E. maxima-infected chickens raised under thermoneutral condition [14][15][16].…”

Section: Introductionmentioning

confidence: 90%

Host transcriptome response to heat stress and Eimeria maxima infection in meat-type chickens

Ghareeb,

Foutz,

Schneiders

et al. 2024

PLoS ONE

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Changes in rectal temperature as a means of assessing heat tolerance and sensitivity in chickens

Poku,

Agyemang-Duah,

Donkor

et al. 2024

Trop Anim Health Prod

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High ambient temperature and relative humidity significantly affect growth and production performance in poultry. Ability of poultry to regulate their core body temperature relative to the ambient temperature depends on the relative nutrient/energy expenditure in maintenance and performance requirements. We hypothesized that changes in rectal temperature corrected for surface area can be used as a measure of heat tolerance/sensitivity. Rectal temperatures of one hundred mixed sex Ross 308 broiler chickens were measured hourly from 6 AM to 6 PM at 24 days of age. The ambient temperature and relative humidity were also measured hourly for the same 12-h period. Body weights were measured at day 24 and 38 days of age. The temperature-humidity index (THI) increased from 77.5 at 6.00 AM and peaked at 83.5 at 3.00 PM. The average rectal temperature increased from 39.90 0 C at 6.00 AM to about 41.30 0 C at 9.00 AM. Thereafter, the average rectal temperature remained constant until 3.00 PM when it began to decline. At 6.00 PM, the rectal temperature had declined to about 40.70 °C. Evaporative heat loss is affected by surface areas and as a result, rectal temperature was corrected for surface area. The change in rectal temperature corrected for surface area was negatively correlated with body weight gain indicating variability in the response of individual chickens exposed to similar THI. This variability was attributed to heat tolerance. It was hypothesized that mismatch between nutrient and energy supply and the partition of nutrient/energy between maintenance of core body temperature and protein synthesis could be reflected on the differences in heat-tolerance and body weight gain in the chicken population. The genetic basis of differences in rectal temperature changes corrected for surface area could be elucidated as a means of developing thermo-tolerant chickens.

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mRNA expression of kidney aquaporins and blood composition of meat-type chickens raised with or without glucose supplementation under cyclic heat or thermoneutral condition

Ariyo,

Kwakye,

Sovi

et al. 2024

Journal of Thermal Biology

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Heat Stress Alters the Effect of Eimeria maxima Infection on Ileal Amino Acids Digestibility and Transporters Expression in Meat-Type Chickens

Cited by 3 publications

References 66 publications

Host transcriptome response to heat stress and Eimeria maxima infection in meat-type chickens

Host transcriptome response to heat stress and Eimeria maxima infection in meat-type chickens

Changes in rectal temperature as a means of assessing heat tolerance and sensitivity in chickens

mRNA expression of kidney aquaporins and blood composition of meat-type chickens raised with or without glucose supplementation under cyclic heat or thermoneutral condition

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