Heat stress and sudden infant death syndrome—Stress gene expression after exposure to moderate heat stress

Rohde, Marianne Cathrine; Corydon, Thomas J.; Hansen, Jakob; Pedersen, Christina Gundgaard; Schmidt, Stinne P.; Gregersen, Niels; Banner, Jytte

doi:10.1016/j.forsciint.2013.06.003

Cited by 9 publications

(7 citation statements)

References 60 publications

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“…In contrast to our results, Rhode et al published a study demonstrating higher expression of hsp70 encoding genes in the SIDS group compared with a control group in cases of thermal stress of approximately 40°C and with different incubation times over several hours [28]. They investigated cultured fibroblasts taken from Achilles tendons at forensic autopsy without studying the corresponding gene products, the hsps.…”

Section: Percentage Of Reddish Stained Structures In Totalcontrasting

confidence: 90%

“…The most investigated risk factor for SIDS is the prone sleeping position [2,[10][11][12]27]. This sleeping position increases the risk of hypercapnia, hypoxia and hyperthermia [3,28]. However, in addition to the inevitable and continual discussion of the consequent obstruction of respiration and cerebral blood flow, a prone sleeping position per se can lead to disturbance of physiological temperature regulation.…”

Section: Discussionmentioning

confidence: 99%

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Hsp27 and 70 expression in the heart, lung and kidney in SIDS

Doberentz¹,

Führing²,

Madea³

2016

RJLM

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Purpose. Hyperthermia is one of the known risk factors for sudden infant death syndrome (SIDS). Hyperthermal stress can induce the heat shock response with increased expression of heat-shock proteins (hsps). Methods. Immunohistochemical staining for hsp27 and hsp70 in the myocardial, pulmonary and renal tissue from 120 SIDS cases and 29 control cases was examined. Results. Hsp70 immunostaining was negative in all investigated organs of both groups. Hsp27 expression was found in the lungs in a few cases at different intensities in the study and control groups. Conclusion. The hypothesis of hyperthermia being a pathogenic factor for SIDS was not supported by immunohistochemical visualization of hsp27 or hsp70. However, when the temperature is below 41.8°C, hsp expression is not induced.

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Section: Percentage Of Reddish Stained Structures In Totalcontrasting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Hsp27 and 70 expression in the heart, lung and kidney in SIDS

Doberentz¹,

Führing²,

Madea³

2016

RJLM

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“…High environmental temperatures may be fatal and are associated with sudden death in animals and humans (1). The incidence of heat-associated mortality is likely to increase with global warming, and with the predicted increase in frequency and intensity of heat waves (2,3).…”

Section: Introductionmentioning

confidence: 99%

Expression profiles of heat shock protein 27 and αB-crystallin and their effects on heat-stressed rat myocardial cells in vitro and in vivo

Tang

Chen

Cheng

et al. 2015

Molecular Medicine Reports

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The present study established a heat-stressed rat heart model, and used an H9c2 myocardial cell line to investigate the expression profiles of heat shock protein (Hsp)27 and αB-crystallin, both in vivo and in vitro. Rats and myocardial cells were subjected to 42 ˚C for 0, 20, 40, 60, 80 or 100 min, following which the mRNA and protein expression levels of Hsp27 and αB-crystallin were measured. Following heat shock, the protein expression levels of Hsp27 and αB-crystallin were significantly decreased in the rat heart cells in vivo, whereas their mRNA levels were significantly increased. The opposing association between the protein and mRNA expression levels of Hsp27 and αB-crystallin suggests that the progression from mRNA into proteins via translation may delayed, or proteins may exist as either oligomers or in the phosphorylated form under heat stress. In vitro, Hsp27 and αB-crystallin exhibited similar reductions in the protein levels at 40 and 60 min, then increased to normal values following 80 min of heat stress. However, the mRNA levels were not consistent with the protein levels. The mRNA levels of Hsp27 and αB-crystallin did however exhibit similar tendencies following 60 min of heat stress. The present study investigated these apparently conflicting results between the in vitro cell line and the in vivo body system. The results demonstrated that the protein and mRNA expression levels of Hsp27 and αB-crystallin exhibited similar trends in vivo and in vitro, respectively. These results were confirmed by analysis with STRING 9.1 software, which indicated that Hsp27 and αB-crystallin are co-expressed in rat myocardial cells. However, the individual cell lines and whole body system exhibited different trends in Hsp27 and αB-crystallin levels prior to and following heat stress, thus require further investigation.

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“…Based on the hypothesis that SIDS may be associated with a decreased ability to respond to external stressors, a PCR analysis of Achilles tendon samples from SIDS cases indicated that HSPA1B (Hsp70) and HSPD1 (Hsp60) expression was increased in response to thermal stress. Furthermore, in SIDS cases where the infant was found in a prone position, lower HSPA1B expression was detected compared with cases where the infant was found on the side or the back ( 21 ). In contrast, an immunohistochemical study investigating the role of hyperthermia as a pathogenic factor for SIDS and Hsp27 expression analysis in the kidney, heart, and lung tissue revealed no meaningful differences between SIDS and control cases.…”

Section: Discussionmentioning

confidence: 92%

Mini Review: The Forensic Value of Heat Shock Proteins

et al. 2022

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Forensic pathologists are routinely confronted with unclear causes of death or related findings. In some instances, difficulties arise in relation to questions posed by criminal investigators or prosecutors. Such scenarios may include questions about wound vitality or cause of death where typical or landmark findings are difficult to ascertain. In addition to the usual examinations required to clarify unclear causes of death or address specific questions, immunohistochemistry and genetic analyses have become increasingly important techniques in this area since their establishment last century. Since then, many studies have determined the usefulness and significance of immunohistochemical and genetic investigations on cellular structures and proteins. For example, these proteins include heat shock proteins (Hsp), which were first described in 1962 and are so called based on their molecular weight. They predominantly act as molecular chaperones with cytoprotective functions that support cell survival under (sub) lethal conditions. They are expressed in specific cellular compartments and have many divergent functions. Central family members include, Hsp 27, 60, and 70. This mini review investigates recent research on the Hsp family, their application range, respective forensic importance, and current limitations and provides an outlook on possible applications within forensic science.

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Heat stress and sudden infant death syndrome—Stress gene expression after exposure to moderate heat stress

Cited by 9 publications

References 60 publications

Hsp27 and 70 expression in the heart, lung and kidney in SIDS

Hsp27 and 70 expression in the heart, lung and kidney in SIDS

Expression profiles of heat shock protein 27 and αB-crystallin and their effects on heat-stressed rat myocardial cells in vitro and in vivo

Mini Review: The Forensic Value of Heat Shock Proteins

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