Heat stress‐induced alterations of antioxidants in the freshwater fish <i>Channa punctata</i> Bloch

Kaur, Manpreet; Atif, Fahim; Ali, Mehboob; Rehman, Hasibur; Raisuddin, Sheikh

doi:10.1111/j.1095-8649.2005.00872.x

Cited by 48 publications

(23 citation statements)

References 44 publications

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“…Furthermore, published work in eelpout and juvenile rainbow trout has shown seasonal reductions in GST activity during the fall-winter seasonal transition (Ronisz et al 1999). The transient increases in GST activity observed in the current study at week 3 may be attributed to increased production of reactive oxygen species at higher temperatures (increasing metabolic rate) eliciting antioxidant responses that may interfere with concurrent thermomodulatory adaptations (Kaur et al 2005). Ration-restricted and fasted fish also exhibit peak GST activities at week 3, but to a lesser extent than seen in control fish.…”

Section: Gst Activitymentioning

confidence: 66%

“…These results suggest GST activity is relatively insensitive to transient temperature changes possibly due to 'positive thermal modulation' whereby Km increases with increasing temperatures (Kennedy et al 1991;Kennedy and Walsh 1994). However, long-term variation in GST activity over seasons has been noted in other aquatic organisms and may be linked to increased metabolic rates at high temperatures (Wilhelm Filho et al 2001;Kaur et al 2005). Furthermore, published work in eelpout and juvenile rainbow trout has shown seasonal reductions in GST activity during the fall-winter seasonal transition (Ronisz et al 1999).…”

Section: Gst Activitymentioning

confidence: 91%

See 1 more Smart Citation

Energy allocations to xenobiotic transport and biotransformation reactions in rainbow trout (Oncorhynchus mykiss) during energy intake restriction

Gourley

Kennedy

2009

Comparative Biochemistry and Physiology Part C: Toxicology &

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Section: Gst Activitymentioning

confidence: 66%

Section: Gst Activitymentioning

confidence: 91%

Energy allocations to xenobiotic transport and biotransformation reactions in rainbow trout (Oncorhynchus mykiss) during energy intake restriction

Gourley

Kennedy

2009

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Recent studies on intertidal bivalves show that critical warming may exacerbate cellular oxidative stress [43]. In many species, the increase in lipid peroxidation and reactive oxygen species concentration in cells following heat stress has already been shown to modify the activity of antioxidant enzymes such as GPx [44]. Non‐selenium glutathione peroxidase is involved in detoxification by reducing fatty acid hydroxyperoxides and H 2 O 2 [45].…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes of the Pacific oysterCrassostrea gigasexposed to prolonged thermal stress

et al. 2007

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The fluctuating thermal nature of the marine environment induces physiological changes in ectotherms that require molecular and gene expression adjustments [1]. Comparative gene expression studies can be used to characterize these adjustments and lead to a better understanding of organismal responses to environmental change. Gene expression datasets can be clustered into groups of genes that represent different compartments of cellular function, and changes in the expression of genes from these clusters can be used to formulate hypotheses as to how different tissues and whole organisms respond to particular biotic or abiotic stresses. Few studies have addressed changes in gene expression in response to temperature variation on marine organisms. Alterations in gene expression have been observed in fish acclimated to constant temperatures and then exposed to daily temperature fluctuations [2] or to a strong heat stress [3]. However, few molecular investigations have focused on the thermal stress response in marine invertebrates [4,5], particularly in the context of global changes and the potential effects on marine invertebrates [6,7].The Pacific oyster Crassostrea gigas is a eurythermic bivalve mollusc that colonizes most of the western coast of Europe. This species prefers sheltered estuarine waters, where it is found in intertidal and shallow subtidal zones. Within their geographic range, oysters typically experience and respond to seasonal temperatures ranging from 4 to 24°C [8]. In the coldest regions inhabited by C. gigas, such as Brittany, Groups of oysters (Crassostrea gigas) were exposed to 25°C for 24 days (controls to 13°C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4°C above the summer seawater temperature encountered in western Brittany, France where the animals were collected. Suppression subtractive hybridization was used to identify specific up-and downregulated genes in gill and mantle tissues after 7-10 and 24 days of exposure. The resulting libraries contain 858 different sequences that potentially represent highly expressed genes in thermally stressed oysters. Expression of 17 genes identified in these libraries was studied using real-time PCR in gills and mantle at different time points over the course of the thermal stress. Differential gene expression levels were much higher in gills than in the mantle, showing that gills are more sensitive to thermal stress. Expression of most transcripts (mainly heat shock proteins and genes involved in cellular homeostasis) showed a high and rapid increase at 3-7 days of exposure, followed by a decrease at 14 days, and a second, less-pronounced increase at 17-24 days. A slow-down in protein synthesis occurred after 24 days of thermal stress.Abbreviations CTSL, cathepsin L; EST, expressed sequence tag; HYPK, Huntingtin-interacting protein K; HSP, heat shock protein; LDH, lactate dehydrogenase; MTA-1, metastasis-associated protein 1; SSH, suppression substractive hybridization.

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“…Although copper is an essential micronutrient, it can accumulate and overload hepatocytes, causing liver injury (Pourahmad and O'Brien, 2000). Kaur et al (2005) showed that 3-h heat treatment (from 20 to 32°C) of Channa punctata modulated antioxidant enzymes -GSH, GST, GR, and CAT -in the liver, gill, and kidney. The authors suggested that although the patterns of the increases/ decreases varied among tissues, the changes were unambiguous.…”

Section: Superoxide Dismutase (Sod)mentioning

confidence: 99%

Combined Effects of Copper and Temperature on Antioxidant Enzymes in the Black Rockfish Sebastes schlegeli

Min¹,

Baeck²,

Kang³

2014

Fisheries and aquatic sciences

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Copper has been widely used to control algae and pathogens in fish culture ponds. However, its toxic effects on fish depend not only on its concentration in the water but also on the water quality. A laboratory experiment was conducted to assess copper toxicity in the black rockfish Sebastes schlegeli using a panel of antioxidant enzymes, including glutathione (GSH), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD), at different levels of copper at three water temperatures (WT, 18, 23, 28°C) for 4 days. After exposure to two copper concentrations (100 and 200 μg/L), GSH levels and GST activities increased significantly, depending on WT (P < 0.05) in the liver, gill, and kidney of the black rockfish. GPx and SOD activities decreased significantly with both increasing WT and copper treatment in the organs of black rockfish (P < 0.05). These changes can be seen as initial responses to temperature stress and as a sustained response to copper exposure. This also indicates that GSH and related enzymes activities were sensitive indexes to stress by toxicants such as copper. The present findings suggest that simultaneous stress due to temperature change and copper exposure can accelerate changes in enzymes activities in the black rockfish. This provides another example of synergism between environmental temperature and pollutants, which may have important implications for the survival of fish in polluted environments during seasonal warming and/or global climate change.

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Heat stress‐induced alterations of antioxidants in the freshwater fish Channa punctata Bloch

Cited by 48 publications

References 44 publications

Energy allocations to xenobiotic transport and biotransformation reactions in rainbow trout (Oncorhynchus mykiss) during energy intake restriction

Energy allocations to xenobiotic transport and biotransformation reactions in rainbow trout (Oncorhynchus mykiss) during energy intake restriction

Identification of differentially expressed genes of the Pacific oysterCrassostrea gigasexposed to prolonged thermal stress

Combined Effects of Copper and Temperature on Antioxidant Enzymes in the Black Rockfish Sebastes schlegeli

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