Heat-Stress-Induced Metabolic Changes and Altered Male Reproductive Function

Hou, Yuanlong; Wang, Xiao Yan; Lei, Zhihai; Ping, Jihui; Liu, Jiajian; Ma, Zhiyu; Zhang, Zheng; Jia, Chunman; Jin, Mei; Li, Xiang; Li, Xiaoliang; Chen, Shaoqiu; Lv, Yingfang; Gao, Yingdong; Wang, Jia; Su, Juan

doi:10.1021/pr501312t

Cited by 28 publications

(15 citation statements)

References 44 publications

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“…Serum and urine samples above were pretreated according to previously published methods (Hou et al, 2015;Zhang et al, 2016). To minimize systematic analytical deviations, the samples were run in the order of "N group-EE group-P group-14w group" alternately.…”

Section: Sample Pretreatment and Gc/tofms Analysismentioning

confidence: 99%

Echinacea purpurea Extract Affects the Immune System, Global Metabolome, and Gut Microbiome in Wistar Rats

Wang¹,

Hou²,

Lv³

et al. 2017

JAS

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Echinacea purpurea extract, a traditional herbal food additive with dual-purpose of medicine and edible material, has been widely used for the treatment and prevention of various infectious diseases, especially for children, old aged and immunocompromised patients. Although there were numerous reports suggested E. purpurea possessed immunostimulatory and antibacterial effects in vitro, the mechanisms underlying remained to be elucidated. This study employed immunologic factors analysis, GC-TOFMS based metabolomics and 16S-rRNA-sequencing microbiome profiling technologies to explore the effects of E. purpurea on young rats, a physiological insufficient immunity status, by compared with pidotimod treatment on young rats and adult animals. E. purpurea treatment significantly increased IL-2, decreased IL-6 and affect immunoglobulins in the spleen of young rats, indicating its promotion of cellular immunity. Both the immunologic factors and the global metabolome of E. purpurea treated young rats were close to the status of mature individuals. Results of 16S-rRNA-sequencing of ileum content together with co-metabolism metabolites demonstrated that E. purpurea changed gut microbiota structure characteristically as a reducing Firmicutes phylum, especially Lactobacillus, and a rising Actinobacteria phylum including Bifidobacterium. The results were concluded that E. purpurea could potentially promote the maturation of immune and metabolism of immature rats, and also affect gut flora structure.

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Section: Sample Pretreatment and Gc/tofms Analysismentioning

confidence: 99%

Echinacea purpurea Extract Affects the Immune System, Global Metabolome, and Gut Microbiome in Wistar Rats

Wang¹,

Hou²,

Lv³

et al. 2017

JAS

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“…The present study also showed that urea levels were increased in the kisspeptin-10 group compared with the control group. According to previous reports, increased GAAs and urea suggests accelerated of protein disintegration for energy compensation[24]. …”

Section: Discussionmentioning

confidence: 91%

“…Serum samples were first derivatized according to published methods with few modifications and analyzed with GC/TOF-MS[23, 24]. Briefly, the two internal standards (10 μL of 0.3 mg/mL L-2- in water and 10 μL of 1 mg/mL heptadecanoic in acid methanol) were spiked into 200-μL serum samples from each rat in sequence, followed by a 30-s vortex and 10-min storage at -20°C.…”

Section: Methodsmentioning

confidence: 99%

The effects of kisspeptin-10 on serum metabolism and myocardium in rats

et al. 2017

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Kisspeptin is a peptide encoded by the Kiss 1 gene and is also called metastin. Previous studies have generally focused on several functions of this peptide, including metastasis, puberty, vasoconstriction and reproduction. However, few studies have focused on the cardiac functions of kisspeptin. In the present study, cardiac histomorphology was observed via TEM (transmission electron microscope) and HE and Masson staining to observe instinctive changes. Serum metabolites levels were also measured and analyzed using GC/TOF-MS after injection with kisspeptin-10. A gene chip was employed to screen the potential genes and pathways in the myocardium at the transcriptional leve, while RT-PCR and Western Blot were conducted to verify the relevant mRNA and protein expression, respectively. Histopathological findings demonstrated that there were many irregular wavy contractions through HE staining and increased fibrosis around the heart cells through Masson staining after treatment with kisspeptin-10. Additionally, the main changes in ultrastructure, including changes in mitochondrial and broken mitochondrial cristae, could be observed with TEM after treatment with kisspeptin-10. The PCA scores plot of the serum metabolites was in the apparent partition after injection of kisspeptin-10. Twenty-six obviously changed metabolites were detected and classified as amino acids, carbohydrate metabolites, organic acids and other metabolites. Furthermore, gene chip analysis showed 1112 differentially expressed genes after treatment with kisspeptin-10, including 330 up-regulated genes and 782 down-regulated genes. These genes were enriched in several signaling pathways related to heart diseases. The RT-PCR result for ITGB8, ITGA4, ITGB7, MYL7, HIF1-α and BNP corresponded with the gene chip assay. Moreover, the upregulated genes ITGB8, ITGA4 and BNP also displayed consistent protein levels in Western Blot results. In summary, these findings suggest that kisspeptin-10 could alter the morphology and structure of myocardial cells, serum metabolite levels, and expression of genes and proteins in heart tissues. Our work determined the profound effects of kisspeptin-10 on the heart, which could further lead to the development of therapeutics related to kisspeptin-10, including antagonists and analogs.

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“…It has been found that the mechanism of temperature regulation in the male scrotum in high-temperature environments will be damaged, resulting in compromised sperm quality and viability [1]. Some studies have shown that testicular hyperthermia can induce spermatogenic cell apoptosis, affect sperm development, and decrease sperm concentration, resulting in spermatogenic disorder [2]. However, in daily life, more and more men are in high temperatures for short or long periods of time due to their occupations [3] (e.g., sedentary desk workers [4], welders [5], drivers [6], and construction workers [7]).…”

Section: Introductionmentioning

confidence: 99%

Duzhong Butiansu Prescription Improves Heat Stress‐Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway

Hou

Yuan

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Duzhong Butiansu (DZBTS) prescription contains many traditional Chinese medicines and has been shown to have a curative effect on male fertility. However, the efficacy and mechanism of DZBTS in the treatment of male infertility induced by heat stress have not been reported. The aim of the present study is to elucidate the effect and mechanism of DZBTS on spermatogenic function of a heat stress model in rats. Methods. Male Wistar rats (280–320 g) were given different doses of DZBTS (0.4853 g/kg/d or 0.9707 g/kg/d), Shengjing capsule (0.56 g/kg/d), or double distilled water for 15 days. A 43°C hot water bath for 30 minutes was used to stimulate the testis of rats. Sperm count, sperm motility, the organ index of kidney and gonadal organs, serum sex hormone levels, and serum oxidising reaction index were measured. Haematoxylin and eosin (HE) staining was used to observe the morphology of the testis and kidney. The expression of Hsp70 in testes was observed by immunofluorescence. The changes in heat stress, reproductive-related protein, and mRNA were measured by western blot assay and RT-qPCR. Results. Heat stress downregulated the levels of sex hormone (P<0.05 or P<0.01) and its receptor androgen receptor (AR) protein expression and mRNA (P<0.01) in rats. Meanwhile, heat stress downregulated the levels of CAMP-responsive element-binding (CREB1) protein and mRNA (P<0.01), which are involved with spermatogenesis. Heat stress also decreased the oxidative damage index. Furthermore, Hsp70 and the heat shock transcription factor 1 (HSF1) protein pathway and mRNA level were overactivated (P<0.05 or P<0.01). Finally, the organ coefficients of the kidney and gonadal organs of rats were decreased. The sperm concentration and motility also decreased significantly (P<0.01). DZBTS could recover these changes induced by heat stress. Conclusions. Our results for the first time have found that DZBTS can improve spermatogenesis disorder in a heat stress model in rats, which may be mainly by regulating AR, sperm regulatory protein CREB1, and the HSF/Hsp70 signaling pathway to decrease oxidative stress.

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Heat-Stress-Induced Metabolic Changes and Altered Male Reproductive Function

Cited by 28 publications

References 44 publications

Echinacea purpurea Extract Affects the Immune System, Global Metabolome, and Gut Microbiome in Wistar Rats

Echinacea purpurea Extract Affects the Immune System, Global Metabolome, and Gut Microbiome in Wistar Rats

The effects of kisspeptin-10 on serum metabolism and myocardium in rats

Duzhong Butiansu Prescription Improves Heat Stress‐Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway

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