Heat treatment and viability assessment by Evans blue in cultured Symbiodinium kawagutii cells

Morera, Claudia; Villanueva, Marco A.

doi:10.1007/s11274-009-9987-4

Cited by 13 publications

(11 citation statements)

References 12 publications

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“…Cell death includes at least two independent modes, that is, apoptosis and necrosis. EB dye was first reported by Gaff and Okong-O'Ogola as a vital stain for plant cells in 1971 [ 85 ]. In their study, EB dye leaked through damaged membranes and stained the dead cells but was excluded by viable cells.…”

Section: Use Of Evans Blue Dye As a Necrosis-avid Agentmentioning

confidence: 99%

Evans Blue Dye: A Revisit of Its Applications in Biomedicine

Yao

Xue

et al. 2018

Contrast Media & Molecular Imaging

123

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Evans blue (EB) dye has owned a long history as a biological dye and diagnostic agent since its first staining application by Herbert McLean Evans in 1914. Due to its high water solubility and slow excretion, as well as its tight binding to serum albumin, EB has been widely used in biomedicine, including its use in estimating blood volume and vascular permeability, detecting lymph nodes, and localizing the tumor lesions. Recently, a series of EB derivatives have been labeled with PET isotopes and can be used as theranostics with a broad potential due to their improved half-life in the blood and reduced release. Some of EB derivatives have even been used in translational applications in clinics. In addition, a novel necrosis-avid feature of EB has recently been reported in some preclinical animal studies. Given all these interesting and important advances in EB study, a comprehensive revisiting of EB has been made in its biomedical applications in the review.

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Section: Use Of Evans Blue Dye As a Necrosis-avid Agentmentioning

confidence: 99%

Evans Blue Dye: A Revisit of Its Applications in Biomedicine

Yao

Xue

et al. 2018

Contrast Media & Molecular Imaging

123

View full text Add to dashboard Cite

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“…A. millepora could not be used as the D-type donor because of significant mortality at the only known location where this species associates almost exclusively with D-type Symbiodinium [36]. Dead cells were stained with Evans blue [53] immediately following extraction, and at 24, 48 and 72 h after extraction. Four replicate counts of the number of stained dead cells vs. unstained live cells were conducted using a haemocytometer and light microscope.…”

Section: Preparation Of Freshly Extracted Symbiodinium Cellsmentioning

confidence: 99%

Infection Dynamics Vary between Symbiodinium Types and Cell Surface Treatments during Establishment of Endosymbiosis with Coral Larvae

et al. 2011

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Symbioses between microbes and higher organisms underpin high diversity in many ecosystems, including coral reefs, however mechanisms underlying the early establishment of symbioses remain unclear. Here we examine the roles of Symbiodinium type and cell surface recognition in the establishment of algal endosymbiosis in the reef-building coral, Acropora tenuis. We found 20-70% higher infection success (proportion of larvae infected) and five-fold higher Symbiodinium abundance in larvae exposed to ITS-1 type C1 compared to ITS-1 type D in the first 96 h following exposure. The highest abundance of Symbiodinium within larvae occurred when C1-type cells were treated with enzymes that modified the 40-100 kD glycome, including glycoproteins and long chain starch residues. Our finding of declining densities of Symbiodinium C1 through time in the presence of intact cell surface molecules supports a role for cell surface recognition molecules in controlling post-phagocytosis processes, leading to rejection of some Symbiodinium types in early ontogeny. Reductions in the densities of unmodified C1 symbionts after 96 h, in contrast to increases in D symbionts may suggest the early

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“…Cell viability and DNA fragmentation and medium pH Cell viability was determined by vital stain Evans Blue method (Morera and Villanueva 2009). Evans blue solution is able to stain only dead cells, while living cells remain unstained.…”

Section: Experimental Conditionsmentioning

confidence: 99%

Antioxidant system in programmed cell death of sycamore (Acer pseudoplatanus L.) cultured cells

et al. 2011

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Reactive oxygen species (ROS) have pleiotropic effects in plants. ROS can lead to cellular damage and death or play key roles in control and regulation of biological processes, such as programmed cell death (PCD). This dual role of ROS, as toxic or signalling molecules, is possible because plant antioxidant system (AS) is able to achieve a tight control over ROS cellular levels, balancing properly their production and scavenging. AS response in plant PCD has been clearly described only in the hypersensitive response in incompatible plant-pathogen interactions and in the senescence process and has not been completely unravelled. In sycamore (Acer pseudoplatanus L.) cultured cells PCD can be induced by Fusicoccin (Fc), Tunicamycin (Tu), and Brefeldin A (Ba). These chemicals induce comparable PCD time course and extent, while H 2 O 2 production is detectable only in Fc-and, to a lesser extent, in Ba-treated cells. In this paper the AS has been investigated during PCD of sycamore cells, measuring the effects of the three inducers on the cellular levels of non-enzymatic and enzymatic antioxidants. Results show that the AS behaviour is different in the PCD induced by the three chemicals. In Fc-treated cells AS is mainly devoted to decrease the concentration of toxic intracellular H 2 O 2 levels. On the contrary, in cells treated with Tu and Ba, the cell redox state is shifted to a more reduced state and the enzymatic AS is partially downregulated, allowing ROS to act as signalling molecules.

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Heat treatment and viability assessment by Evans blue in cultured Symbiodinium kawagutii cells

Cited by 13 publications

References 12 publications

Evans Blue Dye: A Revisit of Its Applications in Biomedicine

Evans Blue Dye: A Revisit of Its Applications in Biomedicine

Infection Dynamics Vary between Symbiodinium Types and Cell Surface Treatments during Establishment of Endosymbiosis with Coral Larvae

Antioxidant system in programmed cell death of sycamore (Acer pseudoplatanus L.) cultured cells

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