Heavy metal accumulation and metallothionein concentration in the frog Rana ridibunda after exposure to chromium or a mixture of chromium and cadmium

Loumbourdis, N. S.; Kostaropoulos, Iason; Theodoropoulou, Basiliki; Kalmanti, Dimitra

doi:10.1016/j.envpol.2006.05.011

Cited by 50 publications

(30 citation statements)

References 32 publications

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“…Our findings were consistent with those observed in Rana esculenta. Thus, specimens collected in areas contaminated with chromium (Fenoglio et al, 2005), similarly to those experimentally exposed to chromium and Cd, only to Cd (Loumbourdis and Vogiatzis, 2002;Loumbourdis et al, 2007), or to mercury (Loumbourdis and Danscher, 2004), showed an increase in the area of Kupffer cell clusters. These cells increase their number and/or size due to the influence of heavy metals.…”

Section: Discussionmentioning

confidence: 99%

“…However, there are few studies on Cd pollution in adult specimens. In Rana ridibunda (Kostaropoulos et al, 2005;Sura et al, 2006;Loumbourdis et al, 2007) and Xenopus laevis (Woodall and Maclean, 1992) the liver and kidney are the target organs for Cd accumulation, which causes various undesirable effects. In Bufo regularis (Hilmy et al, 1986) and Bufo raddei (Zhang et al, 2007), alterations caused by Cd have been reported in several hematological parameters.…”

Section: Introductionmentioning

confidence: 99%

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Histopathological and biochemical changes in the liver, kidney, and bloodof amphibians intoxicated with cadmium

Medina¹,

González²,

Klyver³

et al. 2016

Turk J Biol

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The worldwide decline in amphibian populations has made heavy metal pollution a subject of interest. The aim of this work was to analyze the effect of sublethal doses of cadmium (Cd) on the liver, kidney, and blood of Rhinella arenarum specimens. Serum markers indicative of hepatic injury (gamma glutamyltransferase, alkaline phosphatase, pseudo cholinesterase, and total cholesterol) and markers of renal dysfunction (blood urea nitrogen, serum creatinine, calcium, and glucose) showed variation in animals treated with a 0.5 mg/kg dose compared to controls. Histopathological images revealed alterations in the liver (hepatocyte ballooning and hyperplasia of Kupffer cells) and kidney (renal tubular lumen dilation with tubular necrosis) of animals with abnormal serum markers. The above-mentioned lesions were more evident with the 5 mg/kg dose. With the 0.5 mg/kg dose, hematological values remained normal with the exception of the leukocyte formula. Animals treated with 5 mg/kg showed a significant decrease in both white and red blood cell counts and hematocrit and hemoglobin values. Differential leukocyte counts showed neutrophilia, monocytosis, and lymphopenia. Morphological aberrations were found in white and red blood cells. Results indicated that the evaluation of morphological and functional parameters in kidney, liver, and blood is required in order to monitor amphibian populations exposed to chemical contaminants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histopathological and biochemical changes in the liver, kidney, and bloodof amphibians intoxicated with cadmium

Medina¹,

González²,

Klyver³

et al. 2016

Turk J Biol

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“…The metals (Be, Al, Mn, Fe, Co, Ni and U) that were present in the M pond in concentrations higher than all the benchmark values considered in Table 1, were also found in at least one of the tissues of M animals (Table 2) at significant higher values than those observed for the same tissue in animals from the REF site. Other authors (Loumbourdis et al 2007;Stolyar et al 2008) also observed, for the closely related amphibian Rana ridibunda, that in contaminated sites, with higher values of metals, these organisms tend to present higher accumulation of these elements. The absorption of metals in amphibians might result from various factors previously mentioned.…”

Section: Discussionmentioning

confidence: 79%

“…Cooley and Klaverkamp (2000) obtained similar results for Coregonus clupeaformis, exposed to a U-rich diet, with significant accumulation in bones, scales, intestine, liver and kidney and no significant accumulation in muscle. Typically kidney and liver tend to present higher accumulation of metals due to their role as detoxifying and excretory organs (Fisenne 1994;Farombi et al 2007;Loumbourdis et al 2007;Malik et al 2010). As for bones they tend to accumulate calcium analogs, being thus also a target organ for the accumulation of many metals (Arruda-Neto et al 2004;Drolet-Vives et al 2009).…”

Section: Discussionmentioning

confidence: 97%

Antioxidant response and metal accumulation in tissues of Iberian green frogs (Pelophylax perezi) inhabiting a deactivated uranium mine

et al. 2011

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Human mining activities tend often to generate greatly impacted areas which remain contaminated for long periods of time, giving rise to extreme habitats. Mining sites are usually characterized for the production of metal rich effluents with very low pH. In this work we analyzed physical and chemical parameters of water from a deactivated uranium mine pond (M) and a reference site (REF) as well as their metal content. Furthermore, we determined and compared metal accumulation in liver, kidney, bones, muscle and skin of Pelophylax perezi from REF with P. perezi from M. We also determined the enzymatic activities of glutathione-S-transferases (GSTs), catalase (CAT), glutathione reductase (Gred), and glutathione peroxidase (GPx; both selenium-dependent and selenium-independent) in liver, kidney, lung and heart. Additionally, lipoperoxidation (LPO) was also assessed in the same tissues via thiobarbituric acid reactive substances (TBARS) assay and lactate dehydrogenase (LDH) activity was determined in muscle. Our results revealed that the majority of metals were in higher concentrations in tissues of organisms from M. This trend was especially evident for U whose content reached a difference of 1350 fold between REF and M organisms. None of the organs tested for antioxidant defenses revealed LPO, nonetheless, with exception for liver, all organs from the M frogs presented increased total GPx activity and selenium-dependent GPx. However, this response was significant only for the lung, probably as a consequence of the significant inhibition of CAT upstream and to cope with the subsequent increase in H(2)O(2). Lungs were the organs displaying greater responsiveness of the anti-oxidant stress system in frogs from the uranium mine area.

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“…The effects of contamination may result in shorter body length, lower body mass and malformations of limbs or other organs (Sparling et al 2000). As a result, anurans are increasingly used as bioindicators of contaminant accumulation in pollution studies (Welsh and Ollivier 1998;Johansson et al 2001;Loumbourdis et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the effects of urbanization on trace elements of toe bones

Simon

Puky

Braun

et al. 2011

Environ Monit Assess

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Amphibians, particularly frogs and toads, are increasingly used as bioindicators of contaminant accumulation in pollution studies. We developed an analytical technique to analyse their elemental contents based on a small amount of toe bone samples. This method is environment-friendly as, unlike traditional methods, it is not necessary to kill animals during sampling. Using this technique, we explored the effects of urbanization on the elemental contents of toe bones. Bufo bufo specimens were collected from an urban and two rural ponds. The ratios of Ca and P at the ponds were: 20.5% Ca and 14.6% P at the urban pond and 30.4% and 29.6% Ca, 22.4% and 21.7% P at the rural ponds, respectively. For the other elements, the following percentage ratios were found: 0.7% B, 0.3% Mg and 0.06% Zn at the urban pond and 1.1% and 0.4% B, 0.4% Mg and 0.05% Zn at the rural ponds, respectively. Canonical discriminant analysis indicated the separation of the urban and the rural ponds based on the elemental concentrations of toe bones. Significant differences were found between the concentrations of Ca, P, Mg, B and Zn at the urban and the rural ponds (p<0.05). Anthropogenic activity was found to have effects on the elemental contents of toe bones in the urbanized area. Our study also demonstrated that the developed method was appropriate for the elemental analysis of small samples to assess the effects of urbanization.

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Heavy metal accumulation and metallothionein concentration in the frog Rana ridibunda after exposure to chromium or a mixture of chromium and cadmium

Cited by 50 publications

References 32 publications

Histopathological and biochemical changes in the liver, kidney, and bloodof amphibians intoxicated with cadmium

Histopathological and biochemical changes in the liver, kidney, and bloodof amphibians intoxicated with cadmium

Antioxidant response and metal accumulation in tissues of Iberian green frogs (Pelophylax perezi) inhabiting a deactivated uranium mine

Assessment of the effects of urbanization on trace elements of toe bones

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