Heavy metal distribution in blood, liver and kidneys of Loggerhead (Caretta caretta) and Green (Chelonia mydas) sea turtles from the Northeast Mediterranean Sea

Yi̇pel, Mustafa; Tekeli, İbrahim Ozan; İşler, Cafer Tayer; Altuğ, Muhammed Enes

doi:10.1016/j.marpolbul.2017.08.011

Cited by 27 publications

(30 citation statements)

References 23 publications

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“…Exposure to MeHg has been extensively studied and is well documented in mammals, birds, and fish and includes neurotoxicity, impaired growth and development, reduced reproductive success, liver and kidney damage, and immunomodulation [2,[10][11][12][13][14]. Among other changes associated with exposure to MeHg are gene expression levels linked to oxidative stress, microtubule disruption, mitochondrial dysfunction, endoplasmic reticulum stress, disruption of intracellular calcium levels, programmed cell death, dysregulation of transcription and translation, expression of heat shock proteins, and production of reactive oxygen species (ROS) [2,[14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle

Fernández

Pinzón-Velasco

López-Barrera

et al. 2021

Toxics

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To understand changes in enzyme activity and gene expression as biomarkers of exposure to methylmercury, we exposed loggerhead turtle erythrocytes (RBCs) to concentrations of 0, 1, and 5 mg L−1 of MeHg and de novo transcriptome were assembled using RNA-seq. The analysis of differentially expressed genes (DEGs) indicated that 79 unique genes were dysregulated (39 upregulated and 44 downregulated genes). The results showed that MeHg altered gene expression patterns as a response to the cellular stress produced, reflected in cell cycle regulation, lysosomal activity, autophagy, calcium regulation, mitochondrial regulation, apoptosis, and regulation of transcription and translation. The analysis of DEGs showed a low response of the antioxidant machinery to MeHg, evidenced by the fact that genes of early response to oxidative stress were not dysregulated. The RBCs maintained a constitutive expression of proteins that represented a good part of the defense against reactive oxygen species (ROS) induced by MeHg.

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Section: Introductionmentioning

confidence: 99%

Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle

Fernández

Pinzón-Velasco

López-Barrera

et al. 2021

Toxics

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“…Studies about sea turtles usually associate metal concentrations and organic pollutants with the habitat characteristics (Komoroske et al., 2011; Perrault et al., 2013), describing its biochemical pathways and the tissues and organs of accumulation (Anan et al., 2002; Andreani et al., 2008; Esposito et al., 2020; Franzellitti et al., 2004; Gómez‐Ramírez et al., 2020; Jerez et al., 2010; Yipel et al., 2017) and elimination (Guirlet et al., 2008), making comparisons between tissues (Silva et al., 2014), species (Andreani et al., 2008), and populations, and evaluating the potential lethality of high concentrations of some pollutants (Camacho et al., 2013; Perrault et al., 2013). Some concentration of metal may influence survival in embryonic stages reducing the birthrate and emergence of hatchlings (Perrault et al., 2011).…”

Section: Introductionmentioning

confidence: 99%

Metals in eggs and hatchlings of Dermochelys coriacea (testudines) in Espírito Santo, Brazil and their relation to hatching and emergence success

Freire

Sousa²,

Taufner

et al. 2021

Marine Ecology

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Certain concentration of metal may influence survival in embryonic stages, reducing the birthrate and hatching emergence. This study aimed to evaluate concentration of metal in eggs and hatchlings of Dermochelys coriacea and correlate metal concentrations in eggs with hatching and emergence success. The samples were collected at Biological Reserve Station, ES, Brazil. At the nests' opening, unhatched eggs and stillborn hatchlings were collected to survey the successful hatching and emergence of D. coriacea. The eggs shell, egg content, and dead hatchlings metal concentrations were compared to hatching and emergence success. A positive correlation was found between the concentration of copper (Cu) in the hatchlings and the success of the emergence (r2 = .28, p < .05), and a moderate positive correlation between the concentrations of iron (Fe) and barium (Ba; r2 = .44, p < .05) and success hatching. The concentrations of metals in eggs and hatchlings of D. coriacea in the Espírito Santo are below the levels that could be considered harmful to the species. In summary, there is a positive correlation between metals levels and hatching and emergence success.

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“…Mercury produces lethal and sublethal effects in wildlife, causing different alterations (immunopathological, carcinogenic, teratogenic, neural, cardiovascular, renal, reproductive, and endocrine) [7][8][9][10]. To date, it is known that mercury, interacting with the sulfhydryl groups of cells, generates oxidative stress and alters calcium levels.…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Biomarkers in Erythrocytes of Captive Pre-Juvenile Loggerhead Turtles Following Acute Exposure to Methylmercury

et al. 2020

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This study describes the use of erythrocytes (RBCs) of loggerhead turtles as in vitro models for evaluating their toxicity to methylmercury. Blood samples of loggerhead turtles that were born in the Colombian Caribbean were used. The LC50 of RBCs to methylmercury was determined at 96 h using methylmercury concentrations of 0.5–100 mg L−1. Next, the viability of the RBCs and the activity of the enzymes superoxide dismutase (SOD), glutathione S-transferase (GST), and lipid peroxidation by malondialdehyde (MDA) at 6 and 12 h of exposure to acute concentrations of 0, 1, and 5 mg L−1 were evaluated. The LC50 for loggerhead turtle RBCs was 8.32 mg L−1. The cell viability bioassay of RBCs exposed for 12 h only showed 100% cell viability. Increasing in vitro MeHg concentrations caused a corresponding increase in MDA concentration as well as decreases in the activities of SOD and GST. The RBCs represent an excellent model for ecotoxicological studies and SOD, GST, and MDA are biomarkers of environmental pollution and oxidative stress in loggerhead turtles. This was the first study conducted on loggerhead turtle where the response of RBCs to MeHg-induced oxidative stress is evaluated.

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Heavy metal distribution in blood, liver and kidneys of Loggerhead (Caretta caretta) and Green (Chelonia mydas) sea turtles from the Northeast Mediterranean Sea

Cited by 27 publications

References 23 publications

Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle

Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle

Metals in eggs and hatchlings of Dermochelys coriacea (testudines) in Espírito Santo, Brazil and their relation to hatching and emergence success

Oxidative Stress Biomarkers in Erythrocytes of Captive Pre-Juvenile Loggerhead Turtles Following Acute Exposure to Methylmercury

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