Heavy Metal-Induced Inhibition of Photosynthesis: Targets of in Vivo Heavy Metal Chlorophyll Formation1

Küpper, Hendrik; Šetlík, Ivan; Spiller, Martin; Küpper, Frithjof C.; Prášil, Ondřej

doi:10.1046/j.1529-8817.2002.t01-1-01148.x

Cited by 156 publications

(92 citation statements)

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“…Although Cu is an essential component of several enzymes and other compounds in chloroplasts and mitochondria, 33 it can be toxic at higher concentrations. 34 Last, although we predicted that P would be correlated with metal content in tissues due to physiochemical sorption of phosphate to the ENMs, it was only in root tissue of HL plants exposed to CeO 2 ENMs that we found a relationship. At root Ce concentrations below 100 μg g À1 , P was positively associated with Ce (linear regression, R 2 = 0.870, p < 0.005), but this trend plateaued at higher concentrations.…”

Section: Resultscontrasting

confidence: 53%

Environmental Stresses Increase Photosynthetic Disruption by Metal Oxide Nanomaterials in a Soil-Grown Plant

Conway

Beaulieu

et al. 2015

ACS Nano

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Despite an increasing number of studies over the past decade examining the interactions between plants and engineered nanomaterials (ENMs), very few have investigated the influence of environmental conditions on ENM uptake and toxicity, particularly throughout the entire plant life cycle. In this study, soil-grown herbaceous annual plants (Clarkia unguiculata) were exposed to TiO 2 , CeO 2 , or Cu(OH) 2 ENMs at different concentrations under distinct light and nutrient levels for 8 weeks. Biweekly fluorescence and gas exchange measurements were recorded, and tissue samples from mature plants were analyzed for metal content. During peak growth, exposure to TiO 2 and CeO 2 decreased photosynthetic rate and CO 2 assimilation efficiency of plants grown under high light and nutrient conditions, possibly by disrupting energy transfer from photosystem II (PSII) to the Calvin cycle. Exposure Cu(OH) 2 particles also disrupted photosynthesis but only in plants grown under the most stressful conditions (high light, limited nutrient) likely by preventing the oxidation of a primary PSII reaction center. TiO 2 and CeO 2 followed similar uptake and distribution patterns with concentrations being highest in roots followed by leaves then stems, while Cu(OH) 2 was present at highest concentrations in leaves, likely as ionic Cu. ENM accumulation was highly dependent on both light and nutrient levels and a predictive regression model was developed from these data. These results show that abiotic conditions play an important role in mediating the uptake and physiological impacts of ENMs in terrestrial plants.

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Section: Resultscontrasting

confidence: 53%

Environmental Stresses Increase Photosynthetic Disruption by Metal Oxide Nanomaterials in a Soil-Grown Plant

Conway

Beaulieu

et al. 2015

ACS Nano

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“…It has been suggested that the photosynthetic utilization of light by Thlaspi caerulescens is more sensitive to Cd than is the Calvin Cycle (Küpper et al, 2002). Thus, to determine the capacity of utilization of absorbed light energy, contents and fluorescence of Chl were determined in the current study (Kummerová and Vanová, 2007).…”

Section: Discussionmentioning

confidence: 97%

Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings

Shi

et al. 2013

Journal of Environmental Sciences

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The single and combinational effects of cadmium (Cd) and fluoranthene (FLT) on germination, growth and photosynthesis of soybean seedlings were investigated. Exposure to 5, 10, or 15 mg Cd/L or 1, 5, or 10 mg FLT/L individually or in combination significantly decreased germination vigor (3 days) and final germination rate of soybean seeds, except at 1 and 5 mg FLT/L. The results of two-way ANOVA analysis and the Bliss independence model showed that at lower concentrations of FLT (1 mg/L), the interaction between Cd and FLT on germination was antagonistic, whereas the interaction was synergistic when the concentration of FLT was 5 or 10 mg/L and the concentration of Cd was 15 mg/L. Growth, expressed as dry weight, length of shoot and root, leaf area, and photosynthesis, expressed as net photosynthetic rate, intercellular CO 2 concentration, chlorophyll contents and fluorescence of soybean seedlings were also reduced by exposure to 5 or 10 mg Cd/L or 1 or 5 mg FLT/L, singly or jointly. Significant antagonistic effects of exposure to 5 or 10 mg Cd/L or 1 or 5 mg FLT/L on shoot growth and photosynthesis were observed, whereas synergy and antagonism of Cd and FLT were both observed for root growth.

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“…The reason for copper-triggered pigment loss is that Cu in excess inhibits chlorophyll and carotenoid biosynthesis and retards the incorporation of these pigments in photosystems [4,5,27]. In addition, the substitution of the central magnesium ion in the porphyrin ring of chlorophyll by excess Cu may also damage the chlorophyll synthesizing system [21]. Furthermore, the synthesis of chlorophyll requires iron, thus the Cu-induced Fe deficiency (see Table 1) may contribute to the diminution of chlorophyll concentration and the increment of chl a/b ratio [36].…”

Section: Pigment Composition and Photosynthetic Capacitymentioning

confidence: 99%

Comparing the effects of excess copper in the leaves ofBrassica juncea(L. Czern) andBrassica napus(L.) seedlings: Growth inhibition, oxidative stress and photosynthetic damage

Feigl¹,

Kumar²,

Lehotai³

et al. 2015

Acta Biologica Hungarica

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Hydroponic experiments were conducted to compare the effects of excess copper (Cu) on growth and photosynthesis in young Indian mustard (Brassica juncea) and oilseed rape (Brassica napus). We compared the effects of excess Cu on the two Brassica species at different physiological levels from antioxidant levels to photosynthetic activity. Nine-day-old plants were treated with Cu (10, 25 and 50 µM CuSO 4 ) for 7 and 14 days. Both species took up Cu from the external solution to a similar degree but showed slight root-to-shoot translocation. Furthermore, after seven days of treatment, excess Cu significantly decreased other microelement content, such as iron (Fe) and manganese (Mn), especially in the shoots of B. napus. As a consequence, the leaves of young Brassica napus plants showed decreased concentrations of photosynthetic pigments and more intense growth inhibition; however, accumulation of highly reactive oxygen species (hROS) were not detected. After 14 days of Cu exposure the reduction of Fe and Mn contents and shoot growth proved to be comparable in the two species. Moreover, a significant Cu-induced hROS accumulation was observed in both Brassica species. The diminution in pigment contents and photosynthetic efficiency were more pronounced in B. napus during prolonged Cu exposure. Based on all the parameters, B. juncea appears to be more resistant to excess Cu than B. napus, rendering it a species with higher potential for phytoremediation.Keywords: Brassica juncea -Brassica napus -copper -oxidative stress -phytoremediation -photosynthesis INTRODUCTIONAmong heavy metals, copper in trace amounts is essential for plant life but it becomes toxic at higher concentrations. In excess, this heavy metal seriously inhibits leaf expansion at the early growth stage and it causes changes in physiological processes such as transpiration, photosynthetic electron transport and biosynthesis of chlorophyll [30]. Indeed, photosynthesis is the most heavy metal-sensitive process [1] since * Corresponding author; e-mail address: fglgbr@gmail.com + These authors contributed equally to this work. 206Gábor FeiGl et al. Biologica Hungarica 66, 2015 excess Cu can, inter alia, affect photosynthetic electron transport on the reducing side of PSI at the level of ferredoxin. In addition, it can alter the PSII on the oxidising side by inhibiting the electron transport at P680 as well as by inactivating some PSII reaction centres [41]. A few studies indicate that excess Cu can impair the PSII electron transport on its reducing side by affecting the rate of electron transfer from tyrosine (Y Z ) to the oxidized primary donor P680 + [16]. ActaAt the molecular level, excess Cu is able to induce the formation of reactive oxygen species (ROS) via the Fenton or Haber-Weiss reactions which subsequently damage proteins, nucleic acids and lipids [14]. This effect of excess Cu was supported by the positive correlation between Cu-treatment and the production of hydroxyl radicals in Arabidopsis [9]. Besides, excess Cu can indirectly cause oxida...

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Heavy Metal-Induced Inhibition of Photosynthesis: Targets of in Vivo Heavy Metal Chlorophyll Formation1

Cited by 156 publications

References 0 publications

Environmental Stresses Increase Photosynthetic Disruption by Metal Oxide Nanomaterials in a Soil-Grown Plant

Environmental Stresses Increase Photosynthetic Disruption by Metal Oxide Nanomaterials in a Soil-Grown Plant

Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings

Comparing the effects of excess copper in the leaves ofBrassica juncea(L. Czern) andBrassica napus(L.) seedlings: Growth inhibition, oxidative stress and photosynthetic damage

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