Heavy metal toxicity following apical and basolateral exposure in the human intestinal cell line Caco-2

Rossi, Anna D.; Poverini, R.; Lullo, G. Di; Modesti, Andrea; Modica, A; Scarino, Maria Laura

doi:10.1016/0887-2333(95)00097-6

Cited by 52 publications

(24 citation statements)

References 25 publications

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“…The long-term Caco-2 TC7 model can be considered to simulate repeated low dose exposure to dietary cadmium in humans, leading to accumulation of cadmium. Caco-2 cells, a cell line with enterocytic-like differentiation, have been shown to be suitable for evaluating uptake of various trace metal such as cadmium (Mata et al 1996;Rossi et al 1996;Jumarie et al 1997 ; Table 1. Effect of cadmium exposure on metals accumulation in cadmium long-term exposed Caco-2 TC7 cells (mg/kg).…”

Section: Discussionmentioning

confidence: 99%

Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model

et al. 2006

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The influence of long-term exposure to cadmium (Cd) on essential minerals was investigated using a Caco-2 TC7 cells and a multi-analytical tool: microwave digestion and inductively coupled plasma mass spectrometry. Intracellular levels, effects on cadmium accumulation, distribution, and reference concentration ranges of the following elements were determined: Na, Mg, Ca, Cr, Fe, Mn, Co, Ni, Cu, Zn, Mo, and Cd. Results showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations ranging from 0 to 10 micromol Cd/l for 5 weeks exhibited a significant increase in cadmium accumulation. Furthermore, this accumulation was more marked in cells exposed long-term to cadmium compared with controls, and that this exposure resulted in a significant accumulation of copper and zinc but not of the other elements measured. Interactions of Cd with three elements: zinc, copper, and manganese were particularly studied. Exposed to 30 micromol/l of the element, manganese showed the highest inhibition and copper the lowest on cadmium intracellular accumulation but Zn, Cu, and Mn behave differently in terms of their mutual competition with Cd. Indeed, increasing cadmium in the culture medium resulted in a gradual and significant increase in the accumulation of zinc. There was a significant decrease in manganese from 5 micromol Cd/l exposure, and no variation was observed with copper.

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Section: Discussionmentioning

confidence: 99%

Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model

et al. 2006

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“…The dose of Cd exposure was 50 M CdCl 2 , selected on the basis of data from our preliminary experiment (see Fig. S1 in the supplemental material) and from the literature (22,23) to correspond to a moderate toxic effect of Cd on intestinal epithelial cells.…”

Section: Methodsmentioning

confidence: 99%

Oral Administration of Probiotics Inhibits Absorption of the Heavy Metal Cadmium by Protecting the Intestinal Barrier

Zhai

Tian

Zhang

et al. 2016

Appl Environ Microbiol

170

105

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The heavy metal cadmium (Cd) is an environmental pollutant that causes adverse health effects in humans and animals. Our previous work demonstrated that oral administration of probiotics can significantly inhibit Cd absorption in the intestines of mice, but further evidence is needed to gain insights into the related protection mode. The goal of this study was to evaluate whether probiotics can inhibit Cd absorption through routes other than the Cd binding, with a focus on gut barrier protection. In the in vitro assay, both the intervention and therapy treatments of

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“…Solute transport by the paracellular and/or the transcellular routes creates a transepithelial potential difference, and the electrical resistance depends on the tightness of the seal between the cells (Powell 1981). Although some in vitro studies have shown reduced barrier functionality in various epithelia as a result of exposure to cadmium (Rossi et al 1996;Duizer et al 1999;Gennari et al 2003), the precise molecular mechanisms of its toxicity are not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the sensitivity of different toxicological endpoints in Caco-2 cells after cadmium chloride treatment

Boveri

Pazos

Gennari

et al. 2004

Archives of Toxicology

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The human colorectal adenocarcinoma cell line Caco-2 is a widely used in vitro model of the intestinal barrier. Cadmium chloride (CdCl(2)) is a highly toxic metal compound, ubiquitous in the biosphere, able to enter the food chain and to reach the intestinal epithelium, causing structural and functional damages. The aim of this work was to characterise cadmium toxicity in Caco-2 cells and, in particular, to compare the sensitivity of different endpoints revealing damage both on the epithelial barrier and at the cellular or molecular level. After 24-h exposure of the cells to CdCl(2), lactate dehydrogenase (LDH) leakage showed cadmium-induced cell toxicity, significant from 25 microM CdCl(2) and above, and analysis of different cell death pathways indicated the presence of necrosis after treatment with 50 microM CdCl(2). At the molecular level, we observed an increase in the protective protein heat shock protein 70 (HSP70), starting at 10 microM CdCl(2). At the barrier level, transepithelial electrical resistance (TEER) decreased while paracellular permeability (PCP) significantly increased after the treatment, showing an EC(50) of 6 and 16 microM CdCl(2), respectively, and indicating the loss of barrier integrity. In conclusion, our data reveal that CdCl(2) toxicity in Caco-2 cells can be detected at the barrier level at very low concentrations; also, HSP70 was shown to be a sensitive marker for detecting in vitro cadmium-induced toxicity.

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Heavy metal toxicity following apical and basolateral exposure in the human intestinal cell line Caco-2

Cited by 52 publications

References 25 publications

Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model

Cadmium accumulation and interactions with zinc, copper, and manganese, analysed by ICP-MS in a long-term Caco-2 TC7 cell model

Oral Administration of Probiotics Inhibits Absorption of the Heavy Metal Cadmium by Protecting the Intestinal Barrier

Comparison of the sensitivity of different toxicological endpoints in Caco-2 cells after cadmium chloride treatment

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