Oral Administration of Probiotics Inhibits Absorption of the Heavy Metal Cadmium by Protecting the Intestinal Barrier

Zhai, Qixiao; Tian, Fengwei; Zhang, Hao; Narbad, Arjan; Chen, Wei

doi:10.1128/aem.00695-16

Cited by 170 publications

(113 citation statements)

References 51 publications

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“…Moreover, pro-inflammatory cytokines can activate the NF-kB pathway, which has been identified to be critical to oxidative stress, and then generate reactive oxygen species (ROS) [44]. At the same time, the production of ROS will aggravate the inflammatory process [11]. Oxidative stress may result in loss of tight junction integrity and increased paracellular permeability [45].…”

Section: Discussionmentioning

confidence: 99%

“…Probiotics exhibit a variety of health-promoting functions [10] and there is accumulating evidence that some probiotics have the potential to alleviate metal toxicity [11,12]. Moreover, probiotics can provide benefits to the host gut through a diverse set of mechanisms that include modulation of immune responses, alleviation of oxidative stress and reduce intestinal permeability by maintenance of intestinal barrier integrity by expression and distribution of TJ proteins [13,14,15].…”

Section: Introductionmentioning

confidence: 99%

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Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

Zhai

Tian

et al. 2016

Nutrients

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Aluminum (Al) is a ubiquitous metal that can seriously harm the health of animals and humans. In our previous study, we demonstrated that Lactobacillus plantarum CCFM639 can decrease Al burden in the tissues of mice by inhibiting intestinal Al absorption. The main aim of the present research was to investigate whether the protection by the strain is also associated with enhancement of the intestinal barrier, alleviation of oxidative stress and modulation of the inflammatory response. In an in vitro cell model, two protection modes (intervention and therapy) were examined and the results indicated that L. plantarum CCFM639 alleviated Al-induced cytotoxicity. In a mouse model, L. plantarum CCFM639 treatment was found to significantly alleviate oxidative stress in the intestinal tract, regulate the function of the intestinal mucosal immune system, restore the integrity of tight junction proteins and maintain intestinal permeability. These results suggest that in addition to Al sequestration, L. plantarum CCFM639 can also inhibit Al absorption by protecting the intestinal barrier, alleviating Al-induced oxidative stress and inflammatory response. Therefore, L. plantarum CCFM639 has the potential to be a dietary supplement ingredient that provides protection against Al-induced gut injury.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

Zhai

Tian

et al. 2016

Nutrients

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“…Intestinal toxicity of Cd includes disruption of intercellular junctions, paracellular leakage, and inflammation . More recently, Cd has also been shown to modify gut immunity and the gut microbiota which could have clinical impact in the context of immune diseases such as inflammatory bowel disease . Clearly, a better understanding of the impact that chronic exposure to low levels of Cd may have on the intestinal integrity and function is of primary importance.…”

Section: Introductionmentioning

confidence: 99%

Zinc interference with Cd‐induced hormetic effect in differentiated Caco‐2 cells: Evidence for inhibition downstream ERK activation

Lemaire

Mireault

Jumarie

2019

J Biochem & Molecular Tox

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Cadmium (Cd) is a toxic metal that enters the food chain. Following oral ingestion, the intestinal epithelium represents an effective protective barrier against Cd toxicity, but it is also a target tissue that may accumulate and trap high levels of the ingested metal. Using human enterocytic‐like Caco‐2 cells, we have previously shown that Cd may induce a concentration and time‐dependent increase in 3‐(4,5‐dimethyl‐2‐thiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay (MTT)‐reducing activity in differentiated cultures with correlation to ERK1/2 activation. The present study shows that (a) Zn prevents the Cd‐induced hormesis effect on MTT reduction in a concentration‐dependent manner, without inhibiting Cd‐induced ERK1/2 activation; (b) Zn also induces similar hormetic stimulation of MTT‐reducing activity but without ERK1/2 activation. The effect of both metals was sensitive to inhibitors of translation during protein synthesis. There is evidence for the involvement of reactive oxygen species (ROS) in Cd‐induced ERK1/2 activation. In contrast, the Zn effect on the MTT‐reducing activity would not be triggered by ROS but it would be sensitive to the redox state of the cell. Steps downstream ERK1/2 activation by Cd does not involve eIF4E which is rather downregulated by Cd. In conclusion, Cd and Zn both can modify translation processes during protein synthesis via different signaling cascades with crosstalk, and cross‐inhibition may occur. This phenomenon is observed over a small range of metal concentrations and is characterized by a hormesis‐like response. Considering that the hormetic effect on dehydrogenase activity could reflect an adaptive response to the metals whether cross‐inhibition is beneficial is an open question.

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“…Some of these studies have also demonstrated that the beneficial effects of lactobacilli in alleviating the toxic effects of metals probably involve additional mechanisms beyond the sequestering of metals at the intestinal lumen. These include protection against oxidative stress and enhancement of the intestinal barrier function, leading to a decreased permeability to metals . Current knowledge of the capacity of LAB in protecting humans from metal toxicity is quite limited: a single clinical trial has been carried out in children and pregnant women in an at‐risk population.…”

Section: Introductionmentioning

confidence: 92%

“…Besides the in vitro binding of heavy metals in aqueous solutions, proof of concept experiments have established the efficacy of lactobacilli not only in the removal of Cd in situ from contaminated foods such as fruit juices, but also in in vivo animal trials. For example, Lactobacillus plantarum strains that possess high capacity of Pb, Cd and Cu binding have been assayed in mice . In this model, a reduction in metal tissue accumulation and increased metal fecal excretion has been observed in animals suffering metal chronic exposure through drinking water.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the binding capacity of mercurial species in Lactobacillus strains

et al. 2017

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BACKGROUND Metal sequestration by bacteria has been proposed as a strategy to counteract metal contamination in foodstuffs. Lactobacilli can interact with metals, although studies with important foodborne metals such as inorganic [Hg(II)] or organic (CH3Hg) mercury are lacking. Lactobacilli were evaluated for their potential to bind these contaminants and the nature of the interaction was assessed by the use of metal competitors, chemical and enzymatical treatments, and mutants affected in the cell wall structure. RESULTS Lactobacillus strains efficiently bound Hg(II) and CH3Hg. Mercury binding by Lactobacillus casei BL23 was independent of cell viability. In BL23, both forms of mercury were cell wall bound. Their interaction was not inhibited by cations and it was resistant to chelating agents and protein digestion. Lactobacillus casei mutants affected in genes involved in the modulation of the negative charge of the cell wall anionic polymer lipoteichoic acid showed increased mercury biosorption. In these mutants, mercury toxicity was enhanced compared to wild‐type bacteria. These data suggest that lipoteichoic acid itself or the physicochemical characteristics that it confers to the cell wall play a major role in mercury complexation. CONCLUSION This is the first example of the biosorption of Hg(II) and CH3Hg in lactobacilli and it represents a first step towards their possible use as agents for diminishing mercury bioaccessibility from food at the gastrointestinal tract. © 2017 Society of Chemical Industry

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Oral Administration of Probiotics Inhibits Absorption of the Heavy Metal Cadmium by Protecting the Intestinal Barrier

Cited by 170 publications

References 51 publications

Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

Zinc interference with Cd‐induced hormetic effect in differentiated Caco‐2 cells: Evidence for inhibition downstream ERK activation

Characterization of the binding capacity of mercurial species in Lactobacillus strains

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